RFX1 (NM_002918) Human Tagged ORF Clone

CAT#: RC207872

RFX1 (Myc-DDK-tagged)-Human regulatory factor X, 1 (influences HLA class II expression) (RFX1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419021) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207872 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RFX1 protein (Cat# TP307872). The protein was produced from HEK293T cells transfected with RFX1 cDNA clone (Cat# RC207872) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RFX1
• Synonyms: EFC; RFX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207872 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACACAGGCGTATACTGAGCTACAGGCAGCCCCGCCACCATCCCAGCCGCCACAGGCCCCGCCAC
  AAGCCCAGCCCCAGCCGCCACCGCCACCACCCCCAGCGGCACCCCAGCCCCCGCAGCCACCCACCGCTGC
  TGCCACCCCTCAGCCCCAATATGTCACCGAGCTGCAGAGCCCCCAGCCCCAGGCACAGCCACCGGGTGGC
  CAGAAGCAGTACGTGACGGAGCTCCCGGCTGTACCCGCACCCTCGCAGCCAACCGGTGCACCCACCCCTT
  CGCCTGCACCCCAGCAGTACATCGTGGTCACTGTCTCTGAAGGTGCCATGCGGGCCAGCGAGACAGTGTC
  GGAGGCCAGCCCCGGCTCCACCGCCAGCCAGACCGGCGTTCCTACTCAGGTGGTTCAGCAGGTGCAGGGC
  ACCCAGCAGCGGCTGCTGGTCCAGACGAGCGTGCAGGCCAAGCCAGGCCACGTGTCGCCCCTCCAGCTGA
  CCAACATCCAAGTGCCCCAGCAGGCTCTTCCCACGCAGCGTCTGGTGGTGCAGAGCGCAGCCCCAGGCAG
  CAAAGGTGGCCAGGTCTCCCTGACGGTCCATGGTACCCAGCAGGTGCACTCGCCCCCAGAGCAGTCGCCG
  GTGCAGGCCAACAGCTCTTCCAGCAAGACAGCCGGGGCCCCCACGGGCACAGTGCCACAGCAGCTGCAGG
  TCCACGGCGTCCAGCAGAGTGTCCCCGTCACCCAAGAGAGATCTGTGGTCCAGGCCACTCCACAAGCGCC
  CAAACCCGGCCCGGTGCAGCCGCTGACCGTGCAGGGCCTCCAGCCAGTCCACGTGGCTCAAGAGGTGCAG
  CAGCTCCAGCAGGTGCCCGTCCCACACGTGTACTCCAGCCAGGTGCAGTATGTGGAGGGCGGCGATGCCA
  GCTACACGGCCAGTGCCATCCGTTCCAGCACCTACTCCTATCCCGAGACGCCGCTGTACACGCAGACGGC
  AAGCACCAGCTACTACGAGGCCGCAGGCACGGCCACCCAGGTCAGCACCCCCGCCACCTCCCAGGCGGTG
  GCCAGCAGTGGCTCCATGCCCATGTACGTGTCCGGCAGCCAGGTCGTCGCCAGCTCCGCCAGCACTGGGG
  CTGGGGCCAGCAACAGCAGCGGAGGTGGTGGCAGTGGTGGTGGCGGCGGCGGCGGGGGAGGCGGTGGCGG
  GGGTGGCAGTGGCAGCACCGGAGGCGGCGGCAGCGGAGCAGGCACCTACGTGATCCAAGGCGGCTACATG
  CTGGGCAGTGCCAGCCAGTCTTACTCTCACACCACCCGTGCCTCGCCAGCCACGGTCCAGTGGCTCCTGG
  ACAACTATGAGACGGCTGAGGGCGTGAGTCTGCCACGGAGCACCCTCTACTGCCACTACTTACTGCACTG
  CCAGGAGCAGAAGCTGGAGCCCGTCAACGCCGCCTCCTTCGGCAAGCTCATCCGCTCCGTCTTCATGGGC
  CTGCGAACCCGCCGTCTGGGCACCAGGGGCAACTCCAAGTACCACTACTATGGCCTGCGCATCAAGGCCA
  GCTCACCCCTGCTGCGGCTGATGGAGGACCAGCAGCACATGGCCATGCGGGGCCAGCCCTTCTCGCAGAA
  GCAGAGGCTCAAGCCCATCCAGAAGATGGAAGGCATGACCAACGGCGTGGCGGTGGGGCAGCAGCCGAGC
  ACGGGGCTGTCGGACATCAGCGCCCAGGTGCAGCAGTACCAGCAATTTTTGGATGCCTCTCGGAGCCTCC
  CTGACTTCACAGAGCTCGACCTCCAGGGCAAGGTGCTGCCTGAGGGCGTCGGGCCCGGGGACATCAAAGC
  CTTCCAGGTCCTGTACCGGGAACACTGTGAGGCCATTGTCGACGTCATGGTGAACCTGCAGTTCACCCTG
  GTGGAGACGCTGTGGAAGACCTTCTGGAGGTACAACCTCAGCCAGCCCAGTGAGGCGCCACCGCTGGCTG
  TACATGACGAGGCCGAGAAGCGACTGCCCAAAGCCATCCTGGTGCTCCTCTCCAAGTTCGAGCCCGTGCT
  CCAATGGACCAAGCACTGTGACAACGTGCTGTACCAGGGCCTGGTGGAAATCCTCATTCCCGACGTGCTG
  CGGCCCATCCCCAGTGCCTTGACCCAAGCGATCCGGAACTTTGCCAAGAGCCTGGAGAGCTGGCTCACCC
  ACGCCATGGTCAACATCCCCGAGGAGATGCTGCGGGTGAAGGTGGCCGCGGCTGGCGCCTTCGCGCAGAC
  ACTGCGGCGCTACACGTCGCTCAACCACCTGGCGCAGGCGGCGCGCGCTGTGCTGCAGAACACCGCACAG
  ATCAACCAGATGCTGAGCGACCTCAACCGCGTGGACTTCGCCAACGTGCAGGAGCAGGCCTCGTGGGTGT
  GCCGCTGCGAGGACCGCGTGGTGCAGCGGCTGGAGCAGGACTTCAAGGTGACGCTGCAGCAGCAGAACTC
  GCTGGAGCAGTGGGCGGCCTGGCTGGACGGCGTGGTGAGCCAGGTGCTCAAGCCCTACCAGGGCAGCGCC
  GGCTTCCCCAAGGCCGCCAAGCTCTTCCTCCTCAAGTGGTCCTTCTACAGCTCCATGGTGATCCGGGACC
  TGACCCTGCGCAGCGCCGCCAGCTTCGGTTCCTTCCACCTCATCCGGCTGCTCTACGACGAGTACATGTA
  CTACCTGATCGAGCACCGCGTAGCCCAGGCCAAGGGCGAGACCCCCATCGCCGTCATGGGCGAGTTCGCC
  AATCTGGCCACCTCCCTGAACCCCCTGGACCCCGACAAAGACGAGGAGGAAGAAGAGGAGGAGGAGAGCG
  AGGACGAGCTGCCGCAGGACATCTCACTGGCGGCTGGCGGCGAGTCACCCGCGCTGGGCCCGGAGACCCT
  GGAGCCGCCGGCCAAGCTGGCGCGGACTGACGCGCGCGGCCTCTTCGTGCAGGCGCTGCCCTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207872 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATQAYTELQAAPPPSQPPQAPPQAQPQPPPPPPPAAPQPPQPPTAAATPQPQYVTELQSPQPQAQPPGG
  QKQYVTELPAVPAPSQPTGAPTPSPAPQQYIVVTVSEGAMRASETVSEASPGSTASQTGVPTQVVQQVQG
  TQQRLLVQTSVQAKPGHVSPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSP
  VQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQERSVVQATPQAPKPGPVQPLTVQGLQPVHVAQEVQ
  QLQQVPVPHVYSSQVQYVEGGDASYTASAIRSSTYSYPETPLYTQTASTSYYEAAGTATQVSTPATSQAV
  ASSGSMPMYVSGSQVVASSASTGAGASNSSGGGGSGGGGGGGGGGGGGGSGSTGGGGSGAGTYVIQGGYM
  LGSASQSYSHTTRASPATVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMG
  LRTRRLGTRGNSKYHYYGLRIKASSPLLRLMEDQQHMAMRGQPFSQKQRLKPIQKMEGMTNGVAVGQQPS
  TGLSDISAQVQQYQQFLDASRSLPDFTELDLQGKVLPEGVGPGDIKAFQVLYREHCEAIVDVMVNLQFTL
  VETLWKTFWRYNLSQPSEAPPLAVHDEAEKRLPKAILVLLSKFEPVLQWTKHCDNVLYQGLVEILIPDVL
  RPIPSALTQAIRNFAKSLESWLTHAMVNIPEEMLRVKVAAAGAFAQTLRRYTSLNHLAQAARAVLQNTAQ
  INQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKVTLQQQNSLEQWAAWLDGVVSQVLKPYQGSA
  GFPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFA
  NLATSLNPLDPDKDEEEEEEEESEDELPQDISLAAGGESPALGPETLEPPAKLARTDARGLFVQALPSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002918
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002918.1, NM_002918.2, NM_002918.3, NM_002918.4, NP_002909.3
• RefSeq Size: 4392 bp
• RefSeq ORF: 2940 bp
• Locus ID: 5989
• Cytogenetics: 19p13.12
• Protein Families: Druggable Genome
• MW: 104.7 kDa
• Gene Summary: 'This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016]'