RFX1 (NM_002918) Human Untagged Clone

CAT#: SC324081

RFX1 (untagged)-Human regulatory factor X, 1 (influences HLA class II expression) (RFX1)


  "NM_002918" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RFX1
Synonyms EFC; RFX
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_002918.3 CACGGTAGTCGTGGCGACCAAGCAACCCGGCAACGCGAGTCAACAACAACAACCGCCCGG
CCGACCCCCACCCCCACCCCCCAGGCCCGGCCCGGGGACCCCAGCACGTCCCGTCCCCTT
CCCAACATCGACTCCGAGACCTCCGAAAAGCGTTTCCGCAGACAGAAGTGGGGAGAAGCG
GAGGAATTAAAAAAAAAAAAGCCTTATTTATTATCATTTTCCCCACCGTTGGCATGGCAA
CACAGGCGTATACTGAGCTACAGGCAGCCCCGCCACCATCCCAGCCGCCACAGGCCCCGC
CACAAGCCCAGCCCCAGCCGCCACCGCCACCACCCCCAGCGGCACCCCAGCCCCCGCAGC
CACCCACCGCTGCTGCCACCCCTCAGCCCCAATATGTCACCGAGCTGCAGAGCCCCCAGC
CCCAGGCACAGCCACCGGGTGGCCAGAAGCAGTACGTGACGGAGCTCCCGGCTGTACCCG
CACCCTCGCAGCCAACCGGTGCACCCACCCCTTCGCCTGCACCCCAGCAGTACATCGTGG
TCACTGTCTCTGAAGGTGCCATGCGGGCCAGCGAGACAGTGTCGGAGGCCAGCCCCGGCT
CCACCGCCAGCCAGACCGGCGTTCCTACTCAGGTGGTTCAGCAGGTGCAGGGCACCCAGC
AGCGGCTGCTGGTCCAGACGAGCGTGCAGGCCAAGCCAGGCCACGTGTCGCCCCTCCAGC
TGACCAACATCCAAGTGCCCCAGCAGGCTCTTCCCACGCAGCGTCTGGTGGTGCAGAGCG
CAGCCCCAGGCAGCAAAGGTGGCCAGGTCTCCCTGACGGTCCATGGTACCCAGCAGGTGC
ACTCGCCCCCAGAGCAGTCGCCGGTGCAGGCCAACAGCTCTTCCAGCAAGACAGCCGGGG
CCCCCACGGGCACAGTGCCACAGCAGCTGCAGGTCCACGGCGTCCAGCAGAGTGTCCCCG
TCACCCAAGAGAGATCTGTGGTCCAGGCCACTCCACAAGCGCCCAAACCCGGCCCGGTGC
AGCCGCTGACCGTGCAGGGCCTCCAGCCAGTCCACGTGGCTCAAGAGGTGCAGCAGCTCC
AGCAGGTGCCCGTCCCACACGTGTACTCCAGCCAGGTGCAGTATGTGGAGGGCGGCGATG
CCAGCTACACGGCCAGTGCCATCCGTTCCAGCACCTACTCCTATCCCGAGACGCCGCTGT
ACACGCAGACGGCAAGCACCAGCTACTACGAGGCCGCAGGCACGGCCACCCAGGTCAGCA
CCCCCGCCACCTCCCAGGCGGTGGCCAGCAGTGGCTCCATGCCCATGTACGTGTCCGGCA
GCCAGGTCGTCGCCAGCTCCGCCAGCACTGGGGCTGGGGCCAGCAACAGCAGCGGAGGTG
GTGGCAGTGGTGGTGGCGGCGGCGGCGGGGGAGGCGGTGGCGGGGGTGGCAGTGGCAGCA
CCGGAGGCGGCGGCAGCGGAGCAGGCACCTACGTGATCCAAGGCGGCTACATGCTGGGCA
GTGCCAGCCAGTCTTACTCTCACACCACCCGTGCCTCGCCAGCCACGGTCCAGTGGCTCC
TGGACAACTATGAGACGGCTGAGGGCGTGAGTCTGCCACGGAGCACCCTCTACTGCCACT
ACTTACTGCACTGCCAGGAGCAGAAGCTGGAGCCCGTCAACGCCGCCTCCTTCGGCAAGC
TCATCCGCTCCGTCTTCATGGGCCTGCGAACCCGCCGTCTGGGCACCAGGGGCAACTCCA
AGTACCACTACTATGGCCTGCGCATCAAGGCCAGCTCACCCCTGCTGCGGCTGATGGAGG
ACCAGCAGCACATGGCCATGCGGGGCCAGCCCTTCTCGCAGAAGCAGAGGCTCAAGCCCA
TCCAGAAGATGGAAGGCATGACCAACGGCGTGGCGGTGGGGCAGCAGCCGAGCACGGGGC
TGTCGGACATCAGCGCCCAGGTGCAGCAGTACCAGCAATTTTTGGATGCCTCTCGGAGCC
TCCCTGACTTCACAGAGCTCGACCTCCAGGGCAAGGTGCTGCCTGAGGGCGTCGGGCCCG
GGGACATCAAAGCCTTCCAGGTCCTGTACCGGGAACACTGTGAGGCCATTGTCGACGTCA
TGGTGAACCTGCAGTTCACCCTGGTGGAGACGCTGTGGAAGACCTTCTGGAGGTACAACC
TCAGCCAGCCCAGTGAGGCGCCACCGCTGGCTGTACATGACGAGGCCGAGAAGCGACTGC
CCAAAGCCATCCTGGTGCTCCTCTCCAAGTTCGAGCCCGTGCTCCAATGGACCAAGCACT
GTGACAACGTGCTGTACCAGGGCCTGGTGGAAATCCTCATTCCCGACGTGCTGCGGCCCA
TCCCCAGTGCCTTGACCCAAGCGATCCGGAACTTTGCCAAGAGCCTGGAGAGCTGGCTCA
CCCACGCCATGGTCAACATCCCCGAGGAGATGCTGCGGGTGAAGGTGGCCGCGGCTGGCG
CCTTCGCGCAGACACTGCGGCGCTACACGTCGCTCAACCACCTGGCGCAGGCGGCGCGCG
CTGTGCTGCAGAACACCGCACAGATCAACCAGATGCTGAGCGACCTCAACCGCGTGGACT
TCGCCAACGTGCAGGAGCAGGCCTCGTGGGTGTGCCGCTGCGAGGACCGCGTGGTGCAGC
GGCTGGAGCAGGACTTCAAGGTGACGCTGCAGCAGCAGAACTCGCTGGAGCAGTGGGCGG
CCTGGCTGGACGGCGTGGTGAGCCAGGTGCTCAAGCCCTACCAGGGCAGCGCCGGCTTCC
CCAAGGCCGCCAAGCTCTTCCTCCTCAAGTGGTCCTTCTACAGCTCCATGGTGATCCGGG
ACCTGACCCTGCGCAGCGCCGCCAGCTTCGGTTCCTTCCACCTCATCCGGCTGCTCTACG
ACGAGTACATGTACTACCTGATCGAGCACCGCGTAGCCCAGGCCAAGGGCGAGACCCCCA
TCGCCGTCATGGGCGAGTTCGCCAATCTGGCCACCTCCCTGAACCCCCTGGACCCCGACA
AAGACGAGGAGGAAGAAGAGGAGGAGGAGAGCGAGGACGAGCTGCCGCAGGACATCTCAC
TGGCGGCTGGCGGCGAGTCACCCGCGCTGGGCCCGGAGACCCTGGAGCCGCCGGCCAAGC
TGGCGCGGACTGACGCGCGCGGCCTCTTCGTGCAGGCGCTGCCCTCCAGCTAAGCCCTTG
GCCTCCCCGCCCCACCCGCCCCCGCCACCCCTCCACGCCAGGGTCCCTCAAAGCTTCTGT
GGCTTCGTGGTCACTGCTCCAGCCTCAGCCAGGGCAGGGAGGGGGACTCCGAGACAGGGA
AGACCGGGGCCCTCCTCCCTTATGGGGCCGGCACAATCAGTGGGCTGGGCGGAGCCGCAG
CTGCAAACTCTGACACAAAAGACGTGCCTTAAGGAACCCGCCGCGTGCCCAGGTGCCCCG
CGGGGCATCCAGCTCGGCCCCTTGGCCGCCCCCCTCCCAGGCCCCAGCGTCTTCTCCCCA
GCCCCGTCCTCCCACCACCCCAGGGGGCAGGCAGGCAGGCCCCCTCCCGTGCGAAACTGT
TAACTTATTCAGCTCGCTGGCTTTGCACAGCCTGTCCTGGGCCCAGCCCTGAGCCCCGGG
CCGGCGCAGGTGGGCGTCACCTGGGGACTCCCCACTGTCAGCAGCAGCCCCGGGACACCC
TACCCCCAGCAACCCCACTGCTTCCCCTTCTTGGTATTAAGTGCAATTAAAGCCGTGGGT
GTCGCATCTTCTCCAGCGCTGGGCCCTCCCTTGCCCCCAAGCTCAAGAAGGGGGCACTGC
CCGGCCCGGCTGTGAGGAGGGCGGCAGGGGGCACGGCCTCCAGCTTCCAAAGAGCAGCGA
GCCGCGGAGCCGGGGAGTGCCCACAGACCCCAGCCCCCGCGCCCCCGCCCTCCGCCGCTG
GTTTGTAATGGAACCTTTTCTGTGCCCCTATCTTCCCGGAGACCACGCTTCTCCTCCGTG
CCGTGCGACCCCCACCCCATGACTATTGTGCGATTCGTGAGCGCCGCCCGAGCGGAGTTG
GTAACTTGTTGGGTTTTTTTCCAACCATCATGGCCTTATCTGTTCCAGTTTTCTCAGTGT
TTTCAGCCTGTGAGATAGATGTTTATGGCAAGAAAAAGGAAAAAAAAAATGGAAATCTGA
CCTATTGTATAAAAATCACTATTTTGTGTGCTCCGCGTGCTATAGCTTTTGGGGCGGCCC
TGCCCAGTCCCCGTGCCCACGGGGCTCCCTCTCCCGGTGGTGAAAGTGTCCACACGTGTG
GTAGTCTAGTGTGCCGAGCTGTTTTCTACCTTTTTGTAGTCTTTCTTAAAACAATAAATT
CCGCTGTGAAAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT     
ACCN NM_002918
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002918.3, NP_002909.3
RefSeq Size 4347 bp
RefSeq ORF 2940 bp
Locus ID 5989
Cytogenetics 19p13.12
Protein Families Druggable Genome
Gene Summary 'This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016]'

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