RFX1 (NM_002918) Human Untagged Clone

CAT#: SC324081

RFX1 (untagged)-Human regulatory factor X, 1 (influences HLA class II expression) (RFX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX1
• Synonyms: EFC; RFX
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002918.3 CACGGTAGTCGTGGCGACCAAGCAACCCGGCAACGCGAGTCAACAACAACAACCGCCCGG
  CCGACCCCCACCCCCACCCCCCAGGCCCGGCCCGGGGACCCCAGCACGTCCCGTCCCCTT
  CCCAACATCGACTCCGAGACCTCCGAAAAGCGTTTCCGCAGACAGAAGTGGGGAGAAGCG
  GAGGAATTAAAAAAAAAAAAGCCTTATTTATTATCATTTTCCCCACCGTTGGCATGGCAA
  CACAGGCGTATACTGAGCTACAGGCAGCCCCGCCACCATCCCAGCCGCCACAGGCCCCGC
  CACAAGCCCAGCCCCAGCCGCCACCGCCACCACCCCCAGCGGCACCCCAGCCCCCGCAGC
  CACCCACCGCTGCTGCCACCCCTCAGCCCCAATATGTCACCGAGCTGCAGAGCCCCCAGC
  CCCAGGCACAGCCACCGGGTGGCCAGAAGCAGTACGTGACGGAGCTCCCGGCTGTACCCG
  CACCCTCGCAGCCAACCGGTGCACCCACCCCTTCGCCTGCACCCCAGCAGTACATCGTGG
  TCACTGTCTCTGAAGGTGCCATGCGGGCCAGCGAGACAGTGTCGGAGGCCAGCCCCGGCT
  CCACCGCCAGCCAGACCGGCGTTCCTACTCAGGTGGTTCAGCAGGTGCAGGGCACCCAGC
  AGCGGCTGCTGGTCCAGACGAGCGTGCAGGCCAAGCCAGGCCACGTGTCGCCCCTCCAGC
  TGACCAACATCCAAGTGCCCCAGCAGGCTCTTCCCACGCAGCGTCTGGTGGTGCAGAGCG
  CAGCCCCAGGCAGCAAAGGTGGCCAGGTCTCCCTGACGGTCCATGGTACCCAGCAGGTGC
  ACTCGCCCCCAGAGCAGTCGCCGGTGCAGGCCAACAGCTCTTCCAGCAAGACAGCCGGGG
  CCCCCACGGGCACAGTGCCACAGCAGCTGCAGGTCCACGGCGTCCAGCAGAGTGTCCCCG
  TCACCCAAGAGAGATCTGTGGTCCAGGCCACTCCACAAGCGCCCAAACCCGGCCCGGTGC
  AGCCGCTGACCGTGCAGGGCCTCCAGCCAGTCCACGTGGCTCAAGAGGTGCAGCAGCTCC
  AGCAGGTGCCCGTCCCACACGTGTACTCCAGCCAGGTGCAGTATGTGGAGGGCGGCGATG
  CCAGCTACACGGCCAGTGCCATCCGTTCCAGCACCTACTCCTATCCCGAGACGCCGCTGT
  ACACGCAGACGGCAAGCACCAGCTACTACGAGGCCGCAGGCACGGCCACCCAGGTCAGCA
  CCCCCGCCACCTCCCAGGCGGTGGCCAGCAGTGGCTCCATGCCCATGTACGTGTCCGGCA
  GCCAGGTCGTCGCCAGCTCCGCCAGCACTGGGGCTGGGGCCAGCAACAGCAGCGGAGGTG
  GTGGCAGTGGTGGTGGCGGCGGCGGCGGGGGAGGCGGTGGCGGGGGTGGCAGTGGCAGCA
  CCGGAGGCGGCGGCAGCGGAGCAGGCACCTACGTGATCCAAGGCGGCTACATGCTGGGCA
  GTGCCAGCCAGTCTTACTCTCACACCACCCGTGCCTCGCCAGCCACGGTCCAGTGGCTCC
  TGGACAACTATGAGACGGCTGAGGGCGTGAGTCTGCCACGGAGCACCCTCTACTGCCACT
  ACTTACTGCACTGCCAGGAGCAGAAGCTGGAGCCCGTCAACGCCGCCTCCTTCGGCAAGC
  TCATCCGCTCCGTCTTCATGGGCCTGCGAACCCGCCGTCTGGGCACCAGGGGCAACTCCA
  AGTACCACTACTATGGCCTGCGCATCAAGGCCAGCTCACCCCTGCTGCGGCTGATGGAGG
  ACCAGCAGCACATGGCCATGCGGGGCCAGCCCTTCTCGCAGAAGCAGAGGCTCAAGCCCA
  TCCAGAAGATGGAAGGCATGACCAACGGCGTGGCGGTGGGGCAGCAGCCGAGCACGGGGC
  TGTCGGACATCAGCGCCCAGGTGCAGCAGTACCAGCAATTTTTGGATGCCTCTCGGAGCC
  TCCCTGACTTCACAGAGCTCGACCTCCAGGGCAAGGTGCTGCCTGAGGGCGTCGGGCCCG
  GGGACATCAAAGCCTTCCAGGTCCTGTACCGGGAACACTGTGAGGCCATTGTCGACGTCA
  TGGTGAACCTGCAGTTCACCCTGGTGGAGACGCTGTGGAAGACCTTCTGGAGGTACAACC
  TCAGCCAGCCCAGTGAGGCGCCACCGCTGGCTGTACATGACGAGGCCGAGAAGCGACTGC
  CCAAAGCCATCCTGGTGCTCCTCTCCAAGTTCGAGCCCGTGCTCCAATGGACCAAGCACT
  GTGACAACGTGCTGTACCAGGGCCTGGTGGAAATCCTCATTCCCGACGTGCTGCGGCCCA
  TCCCCAGTGCCTTGACCCAAGCGATCCGGAACTTTGCCAAGAGCCTGGAGAGCTGGCTCA
  CCCACGCCATGGTCAACATCCCCGAGGAGATGCTGCGGGTGAAGGTGGCCGCGGCTGGCG
  CCTTCGCGCAGACACTGCGGCGCTACACGTCGCTCAACCACCTGGCGCAGGCGGCGCGCG
  CTGTGCTGCAGAACACCGCACAGATCAACCAGATGCTGAGCGACCTCAACCGCGTGGACT
  TCGCCAACGTGCAGGAGCAGGCCTCGTGGGTGTGCCGCTGCGAGGACCGCGTGGTGCAGC
  GGCTGGAGCAGGACTTCAAGGTGACGCTGCAGCAGCAGAACTCGCTGGAGCAGTGGGCGG
  CCTGGCTGGACGGCGTGGTGAGCCAGGTGCTCAAGCCCTACCAGGGCAGCGCCGGCTTCC
  CCAAGGCCGCCAAGCTCTTCCTCCTCAAGTGGTCCTTCTACAGCTCCATGGTGATCCGGG
  ACCTGACCCTGCGCAGCGCCGCCAGCTTCGGTTCCTTCCACCTCATCCGGCTGCTCTACG
  ACGAGTACATGTACTACCTGATCGAGCACCGCGTAGCCCAGGCCAAGGGCGAGACCCCCA
  TCGCCGTCATGGGCGAGTTCGCCAATCTGGCCACCTCCCTGAACCCCCTGGACCCCGACA
  AAGACGAGGAGGAAGAAGAGGAGGAGGAGAGCGAGGACGAGCTGCCGCAGGACATCTCAC
  TGGCGGCTGGCGGCGAGTCACCCGCGCTGGGCCCGGAGACCCTGGAGCCGCCGGCCAAGC
  TGGCGCGGACTGACGCGCGCGGCCTCTTCGTGCAGGCGCTGCCCTCCAGCTAAGCCCTTG
  GCCTCCCCGCCCCACCCGCCCCCGCCACCCCTCCACGCCAGGGTCCCTCAAAGCTTCTGT
  GGCTTCGTGGTCACTGCTCCAGCCTCAGCCAGGGCAGGGAGGGGGACTCCGAGACAGGGA
  AGACCGGGGCCCTCCTCCCTTATGGGGCCGGCACAATCAGTGGGCTGGGCGGAGCCGCAG
  CTGCAAACTCTGACACAAAAGACGTGCCTTAAGGAACCCGCCGCGTGCCCAGGTGCCCCG
  CGGGGCATCCAGCTCGGCCCCTTGGCCGCCCCCCTCCCAGGCCCCAGCGTCTTCTCCCCA
  GCCCCGTCCTCCCACCACCCCAGGGGGCAGGCAGGCAGGCCCCCTCCCGTGCGAAACTGT
  TAACTTATTCAGCTCGCTGGCTTTGCACAGCCTGTCCTGGGCCCAGCCCTGAGCCCCGGG
  CCGGCGCAGGTGGGCGTCACCTGGGGACTCCCCACTGTCAGCAGCAGCCCCGGGACACCC
  TACCCCCAGCAACCCCACTGCTTCCCCTTCTTGGTATTAAGTGCAATTAAAGCCGTGGGT
  GTCGCATCTTCTCCAGCGCTGGGCCCTCCCTTGCCCCCAAGCTCAAGAAGGGGGCACTGC
  CCGGCCCGGCTGTGAGGAGGGCGGCAGGGGGCACGGCCTCCAGCTTCCAAAGAGCAGCGA
  GCCGCGGAGCCGGGGAGTGCCCACAGACCCCAGCCCCCGCGCCCCCGCCCTCCGCCGCTG
  GTTTGTAATGGAACCTTTTCTGTGCCCCTATCTTCCCGGAGACCACGCTTCTCCTCCGTG
  CCGTGCGACCCCCACCCCATGACTATTGTGCGATTCGTGAGCGCCGCCCGAGCGGAGTTG
  GTAACTTGTTGGGTTTTTTTCCAACCATCATGGCCTTATCTGTTCCAGTTTTCTCAGTGT
  TTTCAGCCTGTGAGATAGATGTTTATGGCAAGAAAAAGGAAAAAAAAAATGGAAATCTGA
  CCTATTGTATAAAAATCACTATTTTGTGTGCTCCGCGTGCTATAGCTTTTGGGGCGGCCC
  TGCCCAGTCCCCGTGCCCACGGGGCTCCCTCTCCCGGTGGTGAAAGTGTCCACACGTGTG
  GTAGTCTAGTGTGCCGAGCTGTTTTCTACCTTTTTGTAGTCTTTCTTAAAACAATAAATT
  CCGCTGTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002918
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002918.3, NP_002909.3
• RefSeq Size: 4347 bp
• RefSeq ORF: 2940 bp
• Locus ID: 5989
• Cytogenetics: 19p13.12
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016]'