C1orf2 (FAM189B) (NM_198264) Human Tagged ORF Clone

CAT#: RC208052

FAM189B (Myc-DDK-tagged)-Human family with sequence similarity 189, member B (FAM189B), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404992) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208052 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM189B
• Synonyms: C1orf2; COTE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208052 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCCTCGCCTAGTGACTCCAGCCGCTCGCTGACCAGCCGGCCCAGCACCAGGGGCCTTACCCACC
  TCCGCCTCCACCGACCCTGGCTGCAGGCCCTGCTTACGCTGGGGCTGGTCCAAGTGCTCCTGGGCATCCT
  GGTGGTCACCTTCAGCATGGTGGCCTCTTCCGTCACCACCACCGAGAGCATCAAGAGGTCCTGCCCGTCT
  TGGGCTGGGTTCTCGAACCTGCTCTTCAGCGTCTGTGGGCTCACCATTTGTGCCGCTATAATCTGTACAC
  TCTCTGCTATTGTCTGCTGCATCCAAATCTTCTCCCTGGACCTCGTGCATACGCTGGCCCCTGAGCGGTC
  AGTCTCAGGCCCACTGGGACCTCTGGGCTGCACGTCCCCGCCCCCAGCCCCTCTCCTACACACCATGCTG
  GACCTGGAGGAATTTGTCCCGCCTGTGCCCCCACCGCCCTACTATCCCCCAGAGTATACCTGCAGCTCAG
  AAACAGATGCACAGAGCATCACGTACAATGGCTCCATGGACAGCCCAGTGCCCTTGTACCCTACCGATTG
  CCCCCCTTCTTATGAGGCAGTCATGGGACTACGAGGAGACAGCCAGGCCACTCTCTTTGACCCTCAGCTT
  CACGATGGCTCGTGCATCTGTGAACGAGTGGCCTCCATTGTAGACGTGTCCATGGACAGCGGGTCTCTGG
  TGCTGTCAGCCATTGGTGACCTCCCTGGGGGCTCTAGCCCGTCGGAGGACTCGTGCCTGCTGGAGCTGCA
  GGGCTCCGTGCGCTCCGTGGACTACGTTCTCTTTCGCTCCATCCAGCGCAGCCGTGCCGGCTACTGCCTC
  AGCCTGGACTGTGGCCTGCGGGGCCCCTTCGAGGAAAGCCCCCTGCCACGGCGCCCCCCACGGGCTGCCC
  GCTCCTATTCCTGCTCTGCCCCTGAAGCTCCACCCCCACTGGGTGCCCCCACAGCTGCCCGCAGCTGCCA
  CCGGTTGGAGGGCTGGCCGCCCTGGGTGGGACCCTGCTTCCCCGAGCTGAGGCGGCGGGTCCCCCGGGGA
  GGGGGCCGCCCAGCCGCAGCCCCGCCCACCCGAGCCCCGACTCGTCGCTTCAGCGATAGCTCAGGTTCCC
  TCACCCCACCGGGGCACCGGCCTCCTCATCCGGCATCCCCACCACCGCTGCTGCTGCCACGGTCCCACAG
  CGACCCAGGCATCACGACCTCCAGTGACACTGCTGACTTCAGGGACCTTTATACCAAAGTGCTTGAGGAA
  GAAGCTGCTTCTGTTTCCTCTGCAGATACAGGGCTCTGCTCTGAAGCCTGCCTCTTCCGCCTAGCCCGCT
  GCCCTTCCCCCAAGTTGCTACGTGCCCGGTCAGCCGAGAAACGGCGCCCTGTGCCCACCTTCCAAAAAGT
  TCCCCTGCCCTCGGGCCCTGCACCTGCCCACTCCCTGGGGGACCTAAAGGGCAGCTGGCCAGGTCGGGGC
  CTGGTCACTCGTTTCCTCCAGATATCCAGGAAAGCCCCAGACCCCAGTGGGACTGGAGCTCATGGACATA
  AGCAGGTGCCCCGGAGCCTGTGGGGCCGGCCTGGCCGAGAGAGCCTCCACCTTCGCAGCTGCGGAGATCT
  GAGCTCTAGCTCTTCCCTGCGGCGTCTCCTGTCTGGCCGCAGGCTGGAGCGTGGTACCCGCCCCCACAGC
  CTCAGCCTCAACGGGGGCAGCCGGGAGACTGGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208052 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMPSPSDSSRSLTSRPSTRGLTHLRLHRPWLQALLTLGLVQVLLGILVVTFSMVASSVTTTESIKRSCPS
  WAGFSNLLFSVCGLTICAAIICTLSAIVCCIQIFSLDLVHTLAPERSVSGPLGPLGCTSPPPAPLLHTML
  DLEEFVPPVPPPPYYPPEYTCSSETDAQSITYNGSMDSPVPLYPTDCPPSYEAVMGLRGDSQATLFDPQL
  HDGSCICERVASIVDVSMDSGSLVLSAIGDLPGGSSPSEDSCLLELQGSVRSVDYVLFRSIQRSRAGYCL
  SLDCGLRGPFEESPLPRRPPRAARSYSCSAPEAPPPLGAPTAARSCHRLEGWPPWVGPCFPELRRRVPRG
  GGRPAAAPPTRAPTRRFSDSSGSLTPPGHRPPHPASPPPLLLPRSHSDPGITTSSDTADFRDLYTKVLEE
  EAASVSSADTGLCSEACLFRLARCPSPKLLRARSAEKRRPVPTFQKVPLPSGPAPAHSLGDLKGSWPGRG
  LVTRFLQISRKAPDPSGTGAHGHKQVPRSLWGRPGRESLHLRSCGDLSSSSSLRRLLSGRRLERGTRPHS
  LSLNGGSRETGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198264
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198264.1, NP_937995.1
• RefSeq Size: 2921 bp
• RefSeq ORF: 1719 bp
• Locus ID: 10712
• Protein Families: Transmembrane
• MW: 61.1 kDa
• Gene Summary: This gene is located near the gene for the lysosomal enzyme glucosylceramidase; a deficiency in this enzyme is associated with Gaucher disease. The encoded protein has been identified as a potential binding partner of a WW domain-containing protein which is involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]