C1orf2 (FAM189B) (NM_198264) Human Tagged ORF Clone
CAT#: RC208052
FAM189B (Myc-DDK-tagged)-Human family with sequence similarity 189, member B (FAM189B), transcript variant 2
"NM_198264" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FAM189B |
Synonyms | C1orf2; COTE1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208052 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCCCTCGCCTAGTGACTCCAGCCGCTCGCTGACCAGCCGGCCCAGCACCAGGGGCCTTACCCACC TCCGCCTCCACCGACCCTGGCTGCAGGCCCTGCTTACGCTGGGGCTGGTCCAAGTGCTCCTGGGCATCCT GGTGGTCACCTTCAGCATGGTGGCCTCTTCCGTCACCACCACCGAGAGCATCAAGAGGTCCTGCCCGTCT TGGGCTGGGTTCTCGAACCTGCTCTTCAGCGTCTGTGGGCTCACCATTTGTGCCGCTATAATCTGTACAC TCTCTGCTATTGTCTGCTGCATCCAAATCTTCTCCCTGGACCTCGTGCATACGCTGGCCCCTGAGCGGTC AGTCTCAGGCCCACTGGGACCTCTGGGCTGCACGTCCCCGCCCCCAGCCCCTCTCCTACACACCATGCTG GACCTGGAGGAATTTGTCCCGCCTGTGCCCCCACCGCCCTACTATCCCCCAGAGTATACCTGCAGCTCAG AAACAGATGCACAGAGCATCACGTACAATGGCTCCATGGACAGCCCAGTGCCCTTGTACCCTACCGATTG CCCCCCTTCTTATGAGGCAGTCATGGGACTACGAGGAGACAGCCAGGCCACTCTCTTTGACCCTCAGCTT CACGATGGCTCGTGCATCTGTGAACGAGTGGCCTCCATTGTAGACGTGTCCATGGACAGCGGGTCTCTGG TGCTGTCAGCCATTGGTGACCTCCCTGGGGGCTCTAGCCCGTCGGAGGACTCGTGCCTGCTGGAGCTGCA GGGCTCCGTGCGCTCCGTGGACTACGTTCTCTTTCGCTCCATCCAGCGCAGCCGTGCCGGCTACTGCCTC AGCCTGGACTGTGGCCTGCGGGGCCCCTTCGAGGAAAGCCCCCTGCCACGGCGCCCCCCACGGGCTGCCC GCTCCTATTCCTGCTCTGCCCCTGAAGCTCCACCCCCACTGGGTGCCCCCACAGCTGCCCGCAGCTGCCA CCGGTTGGAGGGCTGGCCGCCCTGGGTGGGACCCTGCTTCCCCGAGCTGAGGCGGCGGGTCCCCCGGGGA GGGGGCCGCCCAGCCGCAGCCCCGCCCACCCGAGCCCCGACTCGTCGCTTCAGCGATAGCTCAGGTTCCC TCACCCCACCGGGGCACCGGCCTCCTCATCCGGCATCCCCACCACCGCTGCTGCTGCCACGGTCCCACAG CGACCCAGGCATCACGACCTCCAGTGACACTGCTGACTTCAGGGACCTTTATACCAAAGTGCTTGAGGAA GAAGCTGCTTCTGTTTCCTCTGCAGATACAGGGCTCTGCTCTGAAGCCTGCCTCTTCCGCCTAGCCCGCT GCCCTTCCCCCAAGTTGCTACGTGCCCGGTCAGCCGAGAAACGGCGCCCTGTGCCCACCTTCCAAAAAGT TCCCCTGCCCTCGGGCCCTGCACCTGCCCACTCCCTGGGGGACCTAAAGGGCAGCTGGCCAGGTCGGGGC CTGGTCACTCGTTTCCTCCAGATATCCAGGAAAGCCCCAGACCCCAGTGGGACTGGAGCTCATGGACATA AGCAGGTGCCCCGGAGCCTGTGGGGCCGGCCTGGCCGAGAGAGCCTCCACCTTCGCAGCTGCGGAGATCT GAGCTCTAGCTCTTCCCTGCGGCGTCTCCTGTCTGGCCGCAGGCTGGAGCGTGGTACCCGCCCCCACAGC CTCAGCCTCAACGGGGGCAGCCGGGAGACTGGGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208052 protein sequence
Red=Cloning site Green=Tags(s) MMPSPSDSSRSLTSRPSTRGLTHLRLHRPWLQALLTLGLVQVLLGILVVTFSMVASSVTTTESIKRSCPS WAGFSNLLFSVCGLTICAAIICTLSAIVCCIQIFSLDLVHTLAPERSVSGPLGPLGCTSPPPAPLLHTML DLEEFVPPVPPPPYYPPEYTCSSETDAQSITYNGSMDSPVPLYPTDCPPSYEAVMGLRGDSQATLFDPQL HDGSCICERVASIVDVSMDSGSLVLSAIGDLPGGSSPSEDSCLLELQGSVRSVDYVLFRSIQRSRAGYCL SLDCGLRGPFEESPLPRRPPRAARSYSCSAPEAPPPLGAPTAARSCHRLEGWPPWVGPCFPELRRRVPRG GGRPAAAPPTRAPTRRFSDSSGSLTPPGHRPPHPASPPPLLLPRSHSDPGITTSSDTADFRDLYTKVLEE EAASVSSADTGLCSEACLFRLARCPSPKLLRARSAEKRRPVPTFQKVPLPSGPAPAHSLGDLKGSWPGRG LVTRFLQISRKAPDPSGTGAHGHKQVPRSLWGRPGRESLHLRSCGDLSSSSSLRRLLSGRRLERGTRPHS LSLNGGSRETGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_198264 |
ORF Size | 1716 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198264.1, NP_937995.1 |
RefSeq Size | 2921 bp |
RefSeq ORF | 1719 bp |
Locus ID | 10712 |
Protein Families | Transmembrane |
MW | 61.1 kDa |
Gene Summary | This gene is located near the gene for the lysosomal enzyme glucosylceramidase; a deficiency in this enzyme is associated with Gaucher disease. The encoded protein has been identified as a potential binding partner of a WW domain-containing protein which is involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC124663 | FAM189B (untagged)-Human family with sequence similarity 189, member B (FAM189B), transcript variant 2 |
USD 310.00 |
|
SC324099 | FAM189B (untagged)-Human family with sequence similarity 189, member B (FAM189B), transcript variant 2 |
USD 310.00 |
|
RG208052 | FAM189B (GFP-tagged) - Human family with sequence similarity 189, member B (FAM189B), transcript variant 2 |
USD 460.00 |
|
RC208052L3 | Lenti ORF clone of Human family with sequence similarity 189, member B (FAM189B), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC208052L4 | Lenti ORF clone of Human family with sequence similarity 189, member B (FAM189B), transcript variant 2, mGFP tagged |
USD 620.00 |
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