GUCY1B3 (GUCY1B1) (NM_000857) Human Tagged ORF Clone
CAT#: RC208205L3
- LentiORF®
Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), Myc-DDK-tagged
"NM_000857" in other vectors (7)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GUCY1B1 |
Synonyms | GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC208205).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000857 |
ORF Size | 1857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000857.1, NP_000848.1 |
RefSeq Size | 3261 bp |
RefSeq ORF | 1860 bp |
Locus ID | 2983 |
Cytogenetics | 4q32.1 |
Domains | CYCc |
Protein Families | Druggable Genome |
Protein Pathways | Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction |
MW | 70.5 kDa |
Gene Summary | 'This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119659 | GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
USD 310.00 |
|
SC322151 | GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
USD 310.00 |
|
RC208205 | GUCY1B3 (Myc-DDK-tagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
USD 420.00 |
|
RG208205 | GUCY1B3 (GFP-tagged) - Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3) |
USD 460.00 |
|
RC208205L1 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), Myc-DDK-tagged |
USD 768.00 |
|
RC208205L2 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), mGFP tagged |
USD 620.00 |
|
RC208205L4 | Lenti ORF clone of Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3), mGFP tagged |
USD 620.00 |
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