GUCY1B1 (NM_000857) Human Untagged Clone

CAT#: SC322151

GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1B1
• Synonyms: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322151 GTGGCCGCTGCCGCCTCTGCCTGGGTCCCTTCGGCCGTACCTCTGCGTGGGGGCTGCCTC
  CCCGGCTCCCGGTGCAGACACCATGTACGGATTTGTGAATCACGCCCTGGAGTTGCTGGT
  GATCCGCAATTACGGCCCCGAGGTGTGGGAAGACATCAAAAAAGAGGCACAGTTAGATGA
  AGAAGGACAGTTTCTTGTCAGAATAATATATGATGACTCCAAAACTTATGATTTGGTTGC
  TGCTGCAAGCAAAGTCCTCAATCTCAATGCTGGAGAAATCCTCCAAATGTTTGGGAAGAT
  GTTTTTCGTCTTTTGCCAAGAATCTGGTTATGATACAATCTTGCGTGTCCTGGGCTCTAA
  TGTCAGAGAATTTCTACAGAACCTTGATGCTCTGCACGACCACCTTGCTACCATCTACCC
  AGGAATGCGTGCACCTTCCTTTAGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTT
  GCACTACTACTCAGAGAGAGAAGGACTTCAGGATATTGTCATTGGAATCATCAAAACAGT
  GGCACAACAAATCCATGGCACTGAAATAGACATGAAGGTTATTCAGCAAAGAAATGAAGA
  ATGTGATCATACTCAATTTTTAATTGAAGAAAAAGAGTCAAAAGAAGAGGATTTTTATGA
  AGATCTTGACAGATTTGAAGAAAATGGTACCCAGGAATCACGCATCAGCCCATATACATT
  CTGCAAAGCTTTTCCTTTTCATATAATATTTGACCGGGACCTAGTGGTCACTCAGTGTGG
  CAATGCTATATACAGAGTTCTCCCCCAGCTCCAGCCTGGGAATTGCAGCCTTCTGTCTGT
  CTTCTCGCTGGTTCGTCCTCATATTGATATTAGTTTCCATGGGATCCTTTCTCACATCAA
  TACTGTTTTTGTATTGAGAAGCAAGGAAGGATTGTTGGATGTGGAGAAATTAGAATGTGA
  GGATGAACTGACTGGGACTGAGATCAGCTGCTTACGTCTCAAGGGTCAAATGATCTACTT
  ACCTGAAGCAGATAGCATACTTTTTCTATGTTCACCAAGTGTCATGAACCTGGACGATTT
  GACAAGGAGAGGGCTGTATCTAAGTGACATCCCTCTGCATGATGCCACACGCGATCTTGT
  TCTTTTGGGAGAACAATTTAGAGAGGAATACAAACTCACCCAAGAACTGGAAATCCTCAC
  TGACAGGCTACAGCTCACGTTAAGAGCCCTGGAAGATGAAAAGAAAAAGACAGACACATT
  GCTGTATTCTGTCCTTCCTCCGTCTGTTGCCAATGAGCTGCGGCACAAGCGTCCAGTGCC
  TGCCAAAAGATATGACAATGTGACCATCCTCTTTAGTGGCATTGTGGGCTTCAATGCTTT
  CTGTAGCAAGCATGCATCTGGAGAAGGAGCCATGAAGATCGTCAACCTCCTCAACGACCT
  CTACACCAGATTTGACACACTGACTGATTCCCGGAAAAACCCATTTGTTTATAAGGTGGA
  GACTGTTGGTGACAAGTATATGACAGTGAGTGGTTTACCAGAGCCATGCATTCACCATGC
  ACGATCCATCTGCCACCTGGCCTTGGACATGATGGAAATTGCTGGCCAGGTTCAAGTAGA
  TGGTGAATCTGTTCAGATAACAATAGGGATACACACTGGAGAGGTAGTTACAGGTGTCAT
  AGGACAGCGGATGCCTCGATACTGTCTTTTTGGGAATACTGTCAACCTCACAAGCCGAAC
  AGAAACCACAGGAGAAAAGGGAAAAATAAATGTGTCTGAATATACATACAGATGTCTTAT
  GTCTCCAGAAAATTCAGATCCACAATTCCACTTGGAGCACAGAGGCCCAGTGTCCATGAA
  GGGCAAAAAAGAACCAATGCAAGTTTGGTTTCTATCCAGAAAAAATACAGGAACAGAGGA
  AACAAAGCAGGATGATGACTGAATCTTGGATTGTGGGGTGAAGAGGAGTACAGACTAGGT
  TCCAGTTTTCTCCTAACACGTGCCAAGCCCAGGAGCAGTTCTTCCCTATGGATACAGATT
  TTCTTTTGTCCTTGTCCATTACCCCAAGACTTTCTTCTAGATATATCTCTCACTATCCGT
  TATTCAACCTTAGCTCTGCTTTCTATTACTTTTTAGGCTTTAGTATATTATCTAAAGTTT
  GGCTTTTGATGTGGATGATGTGAGCTTCATGTGTCTTAAAATCTACTACAAGCATTACCT
  AACATGGTGATCTGCAAGTAGTAGGCACCCAATAAATATTTGTTGAATTTAGTTAAATGA
  AACTGAACAGTGTTTGGCCATGTGTATATTTATATCATGTTTACCAAATCTGTTTAGTGT
  TCCACATATATGTATATGTATATTTTAATGACTATAATGTAATAAAGTTTATATCATGTT
  GGTGTATATCATTATAGAAATCATTTTCTAAAGGAGTGAATTCTAAGTTTTAGGGGAAAA
  AATGCAATTTATTTTCAGACTCCCAAAGTAAGAATTAACATATCATGCTAAGAAAATAGT
  GACTATTTTGAAGTATGCTACTTCCCTTTCAGAAATATAGAATACACGTTTCTGTTATTA
  AAGTATTTGATTACTAATTCAAATCATATGGCAATTATAATTCTTCTAAAATGCTATCAT
  TTGTAACTGTATCCCCTGTATTAAATCTCATTAACCACAGGCAGCTGTTACAGAAAGCTG
  CATTGTTTCACATTGAGCTGTTACATTAGTTCAGGCTAAATGTTGGGCGCTCCAACCACA
  TCCAAGAATAAATCTGGAAACACACTGCTGGGATACTGCTGTTAGAGCCCTTCTTGGCCT
  TGTATTCCCAGAAATGAGCTCCCTTTCCTTAGCTTAGAAGAATGTGATTATATCCAGGAC
  ATCATGTTCAGAAAACTTAGTTTACTTTCAGCATAGAATGCATTACTGTTGGAATAATTG
  GCCTCTAGCTCTTAAATGTCTCTGATAACTTATTAATATCTATCTTTATAAAATAGAGTG
  CAACTACTTTTGTGTAAAAATGTTTGCCTTTAAATTTAGTATTTCATATCAGCACATCGA
  TATATGTATAAATGTTCCATGTTAATGTGTAAAAGAGTCTGTAATAAATTATTTTTTTCA
  CGTGTCAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000857
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000857.1, NP_000848.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 1860 bp
• Locus ID: 2983
• Cytogenetics: 4q32.1
• Domains: CYCc
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1.