GUCY1B1 (NM_000857) Human Untagged Clone

CAT#: SC119659

GUCY1B3 (untagged)-Human guanylate cyclase 1, soluble, beta 3 (GUCY1B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GUCY1B1
• Synonyms: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119659 sequence for NM_000857 edited (data generated by NextGen Sequencing)
  ATGTACGGATTTGTGAATCACGCCCTGGAGTTGCTGGTGATCCGCAATTACGGCCCCGAG
  GTGTGGGAAGACATCAAAAAAGAGGCACAGTTAGATGAAGAAGGACAGTTTCTTGTCAGA
  ATAATATATGATGACTCCAAAACTTATGATTTGGTTGCTGCTGCAAGCAAAGTCCTCAAT
  CTCAATGCTGGAGAAATCCTCCAAATGTTTGGGAAGATGTTTTTCGTCTTTTGCCAAGAA
  TCTGGTTATGATACAATCTTGCGTGTCCTGGGCTCTAATGTCAGAGAATTTCTACAGAAC
  CTTGATGCTCTGCACGACCACCTTGCTACCATCTACCCAGGAATGCGTGCACCTTCCTTT
  AGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTTGCACTACTACTCAGAGAGAGAA
  GGACTTCAGGATATTGTCATTGGAATCATCAAAACAGTGGCACAACAAATCCATGGCACT
  GAAATAGACATGAAGGTTATTCAGCAAAGAAATGAAGAATGTGATCATACTCAATTTTTA
  ATTGAAGAAAAAGAGTCAAAAGAAGAGGATTTTTATGAAGATCTTGACAGATTTGAAGAA
  AATGGTACCCAGGAATCACGCATCAGCCCATATACATTCTGCAAAGCTTTTCCTTTTCAT
  ATAATATTTGACCGGGACCTAGTGGTCACTCAGTGTGGCAATGCTATATACAGAGTTCTC
  CCCCAGCTCCAGCCTGGGAATTGCAGCCTTCTGTCTGTCTTCTCGCTGGTTCGTCCTCAT
  ATTGATATTAGTTTCCATGGGATCCTTTCTCACATCAATACTGTTTTTGTATTGAGAAGC
  AAGGAAGGATTGTTGGATGTGGAGAAATTAGAATGTGAGGATGAACTGACTGGGACTGAG
  ATCAGCTGCTTACGTCTCAAGGGTCAAATGATCTACTTACCTGAAGCAGATAGCATACTT
  TTTCTATGTTCACCAAGTGTCATGAACCTGGACGATTTGACAAGGAGAGGGCTGTATCTA
  AGTGACATCCCTCTGCATGATGCCACGCGCGATCTTGTTCTTTTGGGAGAACAATTTAGA
  GAGGAATACAAACTCACCCAAGAACTGGAAATCCTCACTGACAGGCTACAGCTCACGTTA
  AGAGCCCTGGAAGATGAAAAGAAAAAGACAGACACATTGCTGTATTCTGTCCTTCCTCCG
  TCTGTTGCCAATGAGCTGCGGCACAAGCGTCCAGTGCCTGCCAAAAGATATGACAATGTG
  ACCATCCTCTTTAGTGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGA
  GAAGGAGCCATGAAGATCGTCAACCTCCTCAACGACCTCTACACCAGATTTGACACACTG
  ACTGATTCCCGGAAAAACCCATTTGTTTATAAGGTGGAGACTGTTGGTGACAAGTATATG
  ACAGTGAGTGGTTTACCAGAGCCATGCATTCACCATGCACGATCCATCTGCCACCTGGCC
  TTGGACATGATGGAAATTGCTGGCCAGGTTCAAGTAGATGGTGAATCTGTTCAGATAACA
  ATAGGGATACACACTGGAGAGGTAGTTACAGGTGTCATAGGACAGCGGATGCCTCGATAC
  TGTCTTTTTGGGAATACTGTCAACCTCACAAGCCGAACAGAAACCACAGGAGAAAAGGGA
  AAAATAAATGTGTCTGAATATACATACAGATGTCTTATGTCTCCAGAAAATTCAGATCCA
  CAATTCCACTTGGAGCACAGAGGCCCAGTGTCCATGAAGGGCAAAAAAGAACCAATGCAA
  GTTTGGTTTCTATCCAGAAAAAATACAGGAACAGAGGAAACAAAGCAGGATGATGACTGA
  
  Clone variation with respect to NM_000857.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000857 unedited
  GCGGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGCCGCTG
  CCGCCTCTGCCTGGGTCCCTTCGGCCGTACCTCTGCGTGGGGGCTGCCTCCCCGGCTCCC
  GGTGCAGACACCATGTACGGATTTGTGAATCACGCCCTGGAGTTGCTGGTGATCCGCAAT
  TACGGCCCCGAGGTGTGGGAAGACATCAAAAAAGAGGCACAGTTAGATGAAGAAGGACAG
  TTTCTTGTCAGAATAATATATGATGACTCCAAAACTTATGATTTGGTTGCTGCTGCAAGC
  AAAGTCCTCAATCTCAATGCTGGAGAAATCCTCCAAATGTTTGGGAAGATGTTTTTCGTC
  TTTTGCCAAGAATCTGGTTATGATACAATCTTGCGTGTCCTGGGCTCTAATGTCAGAGAA
  TTTCTACAGAACCTTGATGCTCTGCACGACCACCTTGCTACCATCTACCCAGGAATGCGT
  GCACCTTCCTTTAGGTGCACTGATGCAGAAAAGGGCAAAGGACTCATTTTGCACTACTAC
  TCANGAGAGAGAGGACTTCAGGATATTGTCATTGGAATCATCAAAACAGTGGCACAACAA
  ATCCATGGCACTGAAATAGACATGAAGGTTATTCAGCANAGAAATGAAAGATGTGATCAT
  ACTCAATTTTTAAATTGAGAAAAGAGTCAAAAGAGAGGATTTTATGAAGATCTTGACAGA
  TTTGAAGAAATGGTACCCAGGAATCACGCATCAGCCCATATACATTCTGCNAAAGCTTTN
  NCCTTTCATATATATTTGACCGGGNACCTAGTGGTCACTCAGTGTGGCAATGCTATATAC
  AAAGTTCTCCCCAGCTCCAGCCTGGGGATTGCANGCTTCTGGCTGGCTTCTCGCTGGGTC
  GNCCTCATATTGAATTAGGTTCCAGGGGATCCTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000857 unedited
  AGCTATGGACCGGCGGCCGCAATCTAGNAGTCGGTTTTTTTTTTTTTTTTTTTTTTTGAA
  AAAAAAATTTATTACAGACTCTTTTACACATTAACATGGAACATTTATACATATATCGAT
  GTGCTGATATGAAATACTAAATTTAAAGGCAAACATTTTTACACAAAAGTAGTTGCACTC
  TATTTTATAAAGATAGATATTAATAAGTTATCAGAGACATTTAAGAGCTAGAGGCCAATT
  ATTCCAACAGTAATGCATTCTATGCTGAAAGTAAACTAAGTTTTCTGAACATGATGTCCT
  GGATATAATCACATTCTTCTAAGCTAAGGAAAGGGAGCTCATTTCTGGGAATACAAGGCC
  AAGAAGGGCTCTAACAGCAGTATCCCAGCAGTGTGTTTCCAGATTTATTCTTGGATGTGG
  TTGGAGCTCCCAACATTTAGCCTGAACTAATGTAACAGCTCAATGTGAAACAATGCAGCT
  TTCTGTAACAGCTGCCTGTGGTTAATGAGATTTAATACAGGGGATACAGTTACAAATGAT
  AGCATTTTAGAAGAATTATAATTGCCATATGATTTGAATTAGTAATCAAATACTTTAATA
  ACAGAAACGTGTATTCTATATTTCTGAAAGGGAAGTAGCATACTTCAAAATAGTCACTAT
  TTTCTTAGCATGATATGTTAATTCTTACTTTGGGAGTCTGAAAATAATTGCATTTTTTCC
  CCTAAAACTTAGAATTCACTCCTTTAGAAATGATTTCTATAATGATATACACCAACATGA
  TATAAACTTTATTACATTATAGNCATTAAAATATACATATACATATATGTGGGAACACTA
  ACAGATTNGGNTAACATGNATATAATATACACATGGGNCCAACACTGGTNCAGNTNCATT
  TAACTAAAAATCACAATATTTATTGGGTGCCTACTACTTGCAGACACCAGGTTAGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000857
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000857.1, NP_000848.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 1860 bp
• Locus ID: 2983
• Cytogenetics: 4q32.1
• Domains: CYCc
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1.