PREB (NM_013388) Human Tagged ORF Clone

CAT#: RC208351

PREB (Myc-DDK-tagged)-Human prolactin regulatory element binding (PREB)

Product Images

Western blot validation of overexpression lysate (Cat# LY402254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208351 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PREB protein (Cat# TP308351). The protein was produced from HEK293T cells transfected with PREB cDNA clone (Cat# RC208351) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PREB
• Synonyms: SEC12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208351 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGCGCCGGGCGCCAGAGCTGTACCGGGCTCCGTTCCCGTTGTACGCGCTTCAGGTCGACCCCA
  GCACTGGGCTGCTCATCGCTGCGGGCGGAGGAGGCGCCGCCAAGACAGGCATAAAGAATGGCGTGCACTT
  TCTGCAGCTAGAGCTGATTAATGGGCGCTTGAGTGCCTCCTTGCTGCACTCCCATGACACAGAGACACGG
  GCCACCATGAACTTGGCACTGGCTGGTGACATCCTTGCTGCAGGGCAGGATGCCCACTGTCAGCTCCTGC
  GCTTCCAGGCACATCAACAGCAGGGCAACAAGGCAGAGAAGGCCGGTTCCAAGGAGCAGGGGCCTCGACA
  AAGGAAGGGAGCAGCCCCAGCAGAGAAGAAATGTGGAGCGGAAACCCAGCACGAGGGGCTAGAACTCAGG
  GTAGAGAATTTGCAGGCGGTGCAGACAGACTTTAGCTCCGATCCACTGCAGAAAGTTGTGTGCTTCAACC
  ACGATAATACCCTGCTTGCCACTGGAGGAACAGATGGCTACGTCCGTGTCTGGAAGGTGCCCAGCCTGGA
  GAAGGTTCTGGAGTTCAAAGCCCACGAAGGGGAGATTGAAGACCTGGCTTTAGGGCCTGATGGCAAGTTG
  GTAACCGTGGGCCGGGACCTTAAGGCCTCTGTGTGGCAGAAGGATCAGCTGGTGACACAGCTGCACTGGC
  AAGAAAATGGACCCACCTTTTCCAGCACACCTTACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGA
  CCAGCCTGCTGGCCTGCGACTCTTCACAGTGCAAATTCCCCACAAGCGCCTGCGCCAGCCCCCTCCCTGC
  TACCTCACAGCCTGGGATGGCTCCAACTTCTTGCCCCTTCGGACCAAGTCCTGTGGCCATGAAGTCGTCT
  CCTGCCTCGATGTCAGTGAATCCGGCACCTTCCTAGGCCTGGGCACAGTCACTGGCTCTGTTGCCATCTA
  CATAGCTTTCTCTCTCCAGTGCCTCTACTACGTGAGGGAGGCCCATGGCATTGTGGTGACGGATGTGGCC
  TTTCTACCTGAGAAGGGTCGTGGTCCAGAGCTCCTTGGGTCCCATGAAACTGCCCTGTTCTCTGTGGCTG
  TGGACAGTCGTTGCCAGCTGCATCTGTTGCCCTCACGGCGGAGTGTTCCTGTGTGGCTCCTGCTCCTGCT
  GTGTGTCGGGCTTATTATTGTGACCATCCTGCTGCTCCAGAGTGCCTTTCCAGGTTTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208351 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRRRAPELYRAPFPLYALQVDPSTGLLIAAGGGGAAKTGIKNGVHFLQLELINGRLSASLLHSHDTETR
  ATMNLALAGDILAAGQDAHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELR
  VENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIEDLALGPDGKL
  VTVGRDLKASVWQKDQLVTQLHWQENGPTFSSTPYRYQACRFGQVPDQPAGLRLFTVQIPHKRLRQPPPC
  YLTAWDGSNFLPLRTKSCGHEVVSCLDVSESGTFLGLGTVTGSVAIYIAFSLQCLYYVREAHGIVVTDVA
  FLPEKGRGPELLGSHETALFSVAVDSRCQLHLLPSRRSVPVWLLLLLCVGLIIVTILLLQSAFPGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013388
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013388.1, NM_013388.2, NM_013388.3, NM_013388.4, NM_013388.5, NP_037520.1
• RefSeq Size: 2211 bp
• RefSeq ORF: 1254 bp
• Locus ID: 10113
• Domains: WD40
• Protein Families: Transcription Factors, Transmembrane
• MW: 45.5 kDa
• Gene Summary: This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. [provided by RefSeq, Jul 2008]