Dnmt2 (TRDMT1) (NM_004412) Human Tagged ORF Clone

CAT#: RC208363

TRDMT1 (Myc-DDK-tagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401401) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208363 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRDMT1
• Synonyms: DMNT2; DNMT2; MHSAIIP; PUMET; RNMT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208363 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGCT
  GTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAATGAAGTATACAAGTATAATTT
  TCCTCACACACAGTTACTTGCCAAGACAATTGAAGGCATTACACTCGAAGAGTTTGACAGATTATCTTTC
  GATATGATTTTAATGAGCCCTCCCTGCCAGCCATTCACAAGGATTGGCCGGCAGGGTGATATGACTGATT
  CAAGGACGAATAGCTTCTTATATATTCTAGATATTCTCCCAAGATTACAAAAATTACCAAAGTATATTCT
  TTTGGAAAATGTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAATTGT
  GGCTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGGCTACGATATT
  TTCTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGTCAGGTACTTATGGAGTTCCC
  CAAAATTGAATCTGTACATCCACAAAAATATGCAATGGATGTAGAAAATAAAATTCAAGAAAAGAACGTT
  GAACCAAATATTAGCTTTGATGGCAGCATACAGTGTTCTGGAAAAGATGCCATTCTTTTTAAGCTTGAAA
  CTGCAGAAGAAATTCACAGGAAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAAGATTTTCT
  TGAAGATGACACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCTCTTCTGTTA
  GACATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTACATAGAAGGGA
  CAGGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTACAAATCCCTTACCAATTTGTC
  ACAAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTGCGATATTTCACTCCTAAAGAAATAGCAAAT
  CTCCTTGGATTTCCTCCAGAGTTCGGATTTCCTGAGAAGATAACAGTGAAACAGCGTTATCGCCTACTTG
  GAAATAGTCTCAACGTGCATGTAGTAGCTAAACTAATCAAAATCTTATATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208363 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSF
  DMILMSPPCQPFTRIGRQGDMTDSRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENC
  GFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNV
  EPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLL
  DIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIAN
  LLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILYE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004412
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004412.1, NM_004412.2, NM_004412.3, NM_004412.4, NM_004412.5, NM_004412.6, NP_004403.1
• RefSeq Size: 7687 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1787
• Cytogenetics: 10p13
• Domains: DNA_methylase
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• MW: 44.6 kDa
• Gene Summary: 'This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]'