Dnmt2 (TRDMT1) (NM_004412) Human Untagged Clone

CAT#: SC125447

TRDMT1 (untagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRDMT1
• Synonyms: DMNT2; DNMT2; MHSAIIP; PUMET; RNMT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125447 sequence for NM_004412 edited (data generated by NextGen Sequencing)
  ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTG
  AGAGAAAGCTGTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAAT
  GAAGTATACAAGTATAATTTTCCTCACACACAGTTACTTGCCAAGACAATTGAAGGCATT
  ACACTCGAAGAGTTTGACAGATTATCTTTCGATATGATTTTAATGAGCCCTCCCTGCCAG
  CCATTCACAAGGATTGGCCGGCAGGGTGATATGACTGATTCAAGGACGAATAGCTTCTTA
  TATATTCTAGATATTCTCCCAAGATTACAAAAATTACCAAAGTATATTCTTTTGGAAAAT
  GTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAATTGT
  GGCTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGG
  CTACGATATTTTCTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGT
  CAGGTACTTATGGAGTTCCCCAAAATTGAATCTGTACATCCACAAAAATATGCAATGGAT
  GTAGAAAATAAAATTCAAGAAAAGAACGTTGAACCAAATATTAGCTTTGATGGCAGCATA
  CAGTGTTCTGGAAAAGATGCCATTCTTTTTAAGCTTGAAACTGCAGAAGAAATTCACAGG
  AAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAAGATTTTCTTGAAGATGAC
  ACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCTCTTCTGTTA
  GACATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTAC
  ATAGAAGGGACAGGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTAC
  AAATCCCTTACCAATTTGTCACAAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTG
  CGATATTTCACTCCTAAAGAAATAGCAAATCTCCTTGGATTTCCTCCAGAGTTCGGATTT
  CCTGAGAAGATAACAGTGAAACAGCGTTATCGCCTACTTGGAAATAGTCTCAACGTGCAT
  GTAGTAGCTAAACTAATCAAAATCTTATATGAATAA
  
  Clone variation with respect to NM_004412.5
  168 g=>a;210 t=>c;301 c=>t;549 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004412 unedited
  TTTGGAATACGACTCACTATAGGGCGGCCGCGAATTCAGATCTGGTACCGGTCCGGAATT
  CCCGGGATATCGTCGACCCACGCGTCCGCGCGGGGATGGAGCCCCTGCGGGTGCTGGAGC
  TATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGCTGTATACCTGCACAAG
  TGGTGGCTGCCATTGATGTCAACACTGGCGCTAATGAAATATACCAGTTTAATTTTCCTC
  ACACACAATTACCTGGCAAGACAATTGAAGGCATTACACTCCAAGAATTTGACAGAATAT
  CTTTCGATATGATTTTAATGAAGCCCTCCTGCCAGCCATTCACAAAGATTGGCCGGCAGG
  GTGATATGACTGATTCAAGGACGAATAGCTTCTTATATATTCTAGATATTCTCCCAGATT
  ACAAAATTACCAAGTATATTCTTTTGAAATGTTAAGGTTTGAATATCTCTACAGAGACCT
  TTGATACANACATAGAAATTGTGGCTTCAGTACCAAAGTTTTATATCTCCACCTCTCTTG
  CATTCCAAATCAGGCTACATATTTTCTAATGCAAAGCTCAGTCAAAGCCATTACCCTTCA
  GCCCCTGTCAGTACTTATGAGTTCCNCAAATTGATCTGGTCTTCACAAAATTGCATGGAT
  GTAGAAATAAATTCAAGAAAGACGTTGAACAATAN
  
• Restriction Sites: Please inquire
• ACCN: NM_004412
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004412.3, NP_004403.1
• RefSeq Size: 3539 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1787
• Cytogenetics: 10p13
• Domains: DNA_methylase
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (a) represents the longest transcript and encodes the longest isoform (a).