SMC3 (NM_005445) Human Tagged ORF Clone

CAT#: RC208401

SMC3 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 3 (SMC3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMC3
• Synonyms: BAM; BMH; CDLS3; CSPG6; HCAP; SMC3L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208401 representing NM_005445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACATAAAGCAGGTGATTATCCAGGGTTTTCGAAGTTACAGAGATCAAACAATTGTAGATCCCTTCA
  GTTCAAAACATAATGTGATTGTGGGCAGAAATGGATCTGGAAAAAGTAACTTTTTTTATGCAATTCAGTT
  TGTTCTCAGTGATGAGTTTAGTCATCTTCGTCCAGAACAGCGGTTGGCTTTATTGCATGAAGGTACTGGT
  CCTCGTGTTATTTCTGCTTTTGTGGAGATTATTTTTGATAATTCAGACAACCGGTTACCAATCGATAAAG
  AGGAAGTTTCACTTCGAAGAGTTATTGGTGCCAAAAAGGATCAGTATTTCTTAGACAAGAAGATGGTCAC
  GAAAAATGATGTGATGAACCTCCTTGAAAGCGCTGGTTTTTCTCGAAGCAATCCTTATTATATTGTTAAA
  CAAGGAAAGATCAACCAGATGGCAACAGCACCAGATTCTCAGAGATTAAAGCTATTAAGAGAAGTAGCTG
  GTACTAGAGTGTATGACGAACGAAAGGAAGAAAGCATCTCCTTAATGAAAGAAACAGAGGGCAAACGGGA
  AAAAATCAATGAGTTGTTAAAATACATTGAAGAGAGATTACATACTCTAGAGGAAGAAAAGGAAGAACTA
  GCTCAGTATCAGAAGTGGGATAAAATGAGACGAGCCCTGGAATATACCATTTACAATCAGGAACTTAACG
  AGACTCGTGCCAAACTTGATGAGCTTTCTGCTAAGCGAGAGACTAGTGGAGAAAAATCCAGACAATTAAG
  AGATGCTCAGCAGGATGCAAGAGATAAAATGGAGGATATCGAACGCCAAGTTAGAGAATTGAAAACAAAA
  ATTTCAGCTATGAAAGAAGAAAAAGAACAGCTTAGTGCTGAAAGACAAGAGCAGATTAAGCAGAGGACTA
  AGTTGGAGCTTAAAGCCAAGGATTTACAAGATGAACTAGCAGGCAATAGTGAACAAAGGAAACGTTTATT
  AAAAGAGAGGCAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCCAAA
  TTCAACAGTGTGAAAGAGAAAGAAGAACGAGGAATTGCTAGATTGGCTCAAGCTACCCAGGAAAGAACGG
  ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACATCAAAAGAAGAAAGGGATAAGTGGATTAAAAA
  GGAACTCAAGTCTTTAGATCAGGCTATTAATGACAAGAAAAGACAGATTGCTGCTATACATAAGGATTTG
  GAAGACACTGAAGCAAATAAAGAGAAAAATCTGGAGCAGTATAATAAACTGGACCAGGATCTTAATGAAG
  TCAAAGCTCGAGTAGAAGAACTGGACAGAAAATATTACGAAGTAAAAAATAAGAAAGATGAACTACAAAG
  TGAAAGAAACTACTTGTGGAGAGAAGAGAATGCAGAACAGCAAGCACTTGCTGCTAAAAGAGAAGATCTT
  GAAAAGAAGCAACAACTTCTTAGAGCAGCAACAGGAAAGGCCATTTTAAATGGAATAGACAGCATAAACA
  AAGTGCTAGACCACTTCCGTCGAAAAGGAATAAACCAGCATGTTCAAAATGGCTATCATGGTATTGTAAT
  GAATAACTTTGAATGTGAACCAGCTTTCTACACATGCGTGGAAGTCACTGCTGGAAACAGGTTATTTTAT
  CACATTGTTGATTCAGATGAAGTCAGCACGAAGATTTTAATGGAGTTTAATAAAATGAATCTTCCTGGAG
  AGGTTACTTTTCTGCCTCTTAACAAGTTAGATGTCAGGGATACAGCCTATCCTGAAACCAATGATGCTAT
  TCCTATGATCAGCAAACTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTGTTTGGAAAGACT
  CTTATTTGTCGTAGCATGGAAGTTTCAACCCAGCTGGCCCGTGCTTTCACTATGGACTGTATTACTTTGG
  AAGGTGACCAAGTCAGCCATCGGGGTGCTCTAACTGGGGGTTATTATGACACAAGGAAGTCTCGACTTGA
  ATTGCAAAAAGATGTTAGAAAAGCAGAAGAAGAACTAGGTGAACTTGAAGCAAAGCTCAATGAAAACCTG
  CGCAGAAATATTGAAAGGATTAATAATGAAATTGATCAGTTGATGAACCAAATGCAACAGATCGAGACCC
  AGCAAAGGAAATTTAAAGCATCTAGAGATAGCATATTATCAGAAATGAAGATGCTAAAAGAGAAGAGGCA
  GCAGTCAGAGAAAACCTTCATGCCTAAGCAACGTAGCTTACAGAGTTTGGAGGCAAGCTTGCATGCTATG
  GAGTCTACCAGAGAGTCATTGAAAGCAGAACTGGGAACTGATTTGCTTTCTCAACTGAGTTTGGAAGATC
  AGAAGAGAGTAGATGCACTGAATGATGAGATTCGTCAACTTCAGCAGGAAAACAGACAGTTGCTAAATGA
  AAGAATTAAATTAGAAGGTATTATTACTCGAGTAGAGACTTATCTCAATGAGAATCTGAGAAAACGCTTG
  GACCAAGTAGAACAGGAACTTAATGAGCTGAGAGAGACAGAAGGGGGTACTGTTCTCACAGCCACAACAT
  CAGAACTTGAAGCCATCAATAAAAGAGTAAAAGACACTATGGCACGATCAGAAGATTTGGACAATTCCAT
  TGATAAAACAGAAGCTGGAATTAAGGAGCTTCAGAAGAGTATGGAGCGCTGGAAAAATATGGAAAAAGAA
  CATATGGATGCTATAAATCATGATACTAAAGAACTGGAAAAGATGACAAATCGGCAAGGCATGCTATTGA
  AGAAGAAAGAAGAGTGTATGAAGAAAATTCGAGAACTTGGATCACTTCCCCAGGAAGCATTTGAAAAGTA
  CCAGACACTGAGCCTCAAACAGTTGTTTCGAAAACTTGAGCAGTGCAACACAGAATTAAAGAAGTACAGC
  CATGTTAACAAAAAGGCTTTGGATCAGTTTGTAAATTTCTCCGAGCAGAAAGAAAAGTTAATAAAGCGTC
  AAGAAGAGTTAGATAGGGGTTACAAATCAATCATGGAACTGATGAATGTACTTGAACTTCGGAAATATGA
  AGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTATTCCAGAAGTTAGTACCTGGT
  GGCAAAGCTACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGTCAGTCTCAAGATGAAGGAGAAGGGA
  GTGGTGAGAGTGAGAGGGGTTCTGGCTCACAAAGCAGTGTCCCATCAGTTGACCAGTTTACTGGAGTTGG
  AATTAGGGTGTCATTTACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAGGTGGACAGAAA
  TCCTTGGTAGCCCTTGCTCTGATTTTTGCCATTCAGAAATGTGACCCGGCTCCATTTTACTTGTTTGATG
  AAATTGACCAGGCTCTGGATGCTCAGCACAGAAAGGCTGTGTCAGATATGATTATGGAACTTGCTGTACA
  TGCTCAGTTTATTACAACTACTTTTAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAAAG
  TTCAGAAATAAGGTTAGTCATATTGATGTGATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGATGATA
  CCACACATGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208401 representing NM_005445
  Red=Cloning site Green=Tags(s)
  
  MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTG
  PRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVK
  QGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEEL
  AQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTK
  ISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPK
  FNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDL
  EDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDL
  EKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFY
  HIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKT
  LICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENL
  RRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAM
  ESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRL
  DQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKE
  HMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYS
  HVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPG
  GKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQK
  SLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVK
  FRNKVSHIDVITAEMAKDFVEDDTTHG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005445
• ORF Size: 3651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005445.1, NM_005445.2, NM_005445.3, NP_005436.1
• RefSeq Size: 4131
• RefSeq ORF: 3654
• Locus ID: 9126
• Domains: SMC_N, SMC_C
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis
• MW: 142 kDa
• Gene Summary: This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]