SMC3 (NM_005445) Human Untagged Clone

CAT#: SC116740

SMC3 (untagged)-Human structural maintenance of chromosomes 3 (SMC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMC3
• Synonyms: BAM; BMH; CDLS3; CSPG6; HCAP; SMC3L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116740 sequence for NM_005445 edited (data generated by NextGen Sequencing)
  ATGTACATAAAGCAGGTGATTATCCAGGGTTTTCGAAGTTACAGAGATCAAACAATTGTA
  GATCCCTTCAGTTCAAAACATAATGTGATTGTGGGCAGAAATGGATCTGGAAAAAGTAAC
  TTTTTTTATGCAATTCAGTTTGTTCTCAGTGATGAGTTTAGTCATCTTCGTCCAGAACAG
  CGGTTGGCTTTATTGCATGAAGGTACTGGTCCTCGTGTTATTTCTGCTTTTGTGGAGATT
  ATTTTTGATAATTCAGACAACCGGTTACCAATCGATAAAGAGGAAGTTTCACTTCGAAGA
  GTTATTGGTGCCAAAAAGGATCAGTATTTCTTAGACAAGAAGATGGTCACGAAAAATGAT
  GTGATGAACCTCCTTGAAAGCGCTGGTTTTTCTCGAAGCAATCCTTATTATATTGTTAAA
  CAAGGAAAGATCAACCAGATGGCAACAGCACCAGATTCTCAGAGATTAAAGCTATTAAGA
  GAAGTAGCTGGTACTAGAGTGTATGACGAACGAAAGGAAGAAAGCATCTCCTTAATGAAA
  GAAACAGAGGGCAAACGGGAAAAAATCAATGAGTTGTTAAAATACATTGAAGAGAGATTA
  CATACTCTAGAGGAAGAAAAGGAAGAACTAGCTCAGTATCAGAAGTGGGATAAAATGAGA
  CGAGCCCTGGAATATACCATTTACAATCAGGAACTTAACGAGACTCGTGCCAAACTTGAT
  GAGCTTTCTGCTAAGCGAGAGACTAGTGGAGAAAAATCCAGACAATTAAGAGATGCTCAG
  CAGGATGCAAGAGATAAAATGGAGGATATCGAACGCCAAGTTAGAGAATTGAAAACAAAA
  ATTTCAGCTATGAAAGAAGAAAAAGAACAGCTTAGTGCTGAAAGACAAGAGCAGATTAAG
  CAGAGGACTAAGTTGGAGCTTAAAGCCAAGGATTTACAAGATGAACTAGCAGGCAATAGT
  GAACAAAGGAAACGTTTATTAAAAGAGAGGCAGAAGCTGCTTGAAAAAATAGAAGAAAAG
  CAGAAAGAACTGGCAGAAACAGAACCCAAATTCAACAGTGTGAAAGAGAAAGAAGAACGA
  GGAATTGCTAGATTGGCTCAAGCTACCCAGGAAAGAACGGATCTTTATGCAAAGCAGGGT
  CGAGGAAGCCAGTTTACATCAAAAGAAGAAAGGGATAAGTGGATTAAAAAGGAACTCAAG
  TCTTTAGATCAGGCTATTAATGACAAGAAAAGACAGATTGCTGCTATACATAAGGATTTG
  GAAGACACTGAAGCAAATAAAGAGAAAAATCTGGAGCAGTATAATAAACTGGACCAGGAT
  CTTAATGAAGTCAAAGCTCGAGTAGAAGAACTGGACAGAAAATATTACGAAGTAAAAAAT
  AAGAAAGATGAACTACAAAGTGAAAGAAACTACTTGTGGAGAGAAGAGAATGCAGAACAG
  CAAGCACTTGCTGCTAAAAGAGAAGATCTTGAAAAGAAGCAACAACTTCTTAGAGCAGCA
  ACAGGAAAGGCCATTTTAAATGGAATAGACAGCATAAACAAAGTGCTAGACCACTTCCGT
  CGAAAAGGAATAAACCAGCATGTTCAAAATGGCTATCATGGTATTGTAATGAATAACTTT
  GAATGTGAACCAGCTTTCTACACATGCGTGGAAGTCACTGCTGGAAACAGGTTATTTTAT
  CACATTGTTGATTCAGATGAAGTCAGCACGAAGATTTTAATGGAGTTTAATAAAATGAAT
  CTTCCTGGAGAGGTTACTTTTCTGCCTCTTAACAAGTTAGATGTCAGGGATACAGCCTAT
  CCTGAAACCAATGATGCTATTCCTATGATCAGCAAACTGAGGTACAATCCCAGATTTGAC
  AAAGCTTTCAAACATGTGTTTGGAAAGACTCTTATTTGTCGTAGCATGGAAGTTTCAACC
  CAGCTGGCCCGTGCTTTCACTATGGACTGTATTACTTTGGAAGGTGACCAAGTCAGCCAT
  CGGGGTGCTCTAACTGGGGGTTATTATGACACAAGGAAGTCTCGACTTGAATTGCAAAAA
  GATGTTAGAAAAGCAGAAGAAGAACTAGGTGAACTTGAAGCAAAGCTCAATGAAAACCTG
  CGCAGAAATATTGAAAGGATTAATAATGAAATTGATCAGTTGATGAACCAAATGCAACAG
  ATCGAGACCCAGCAAAGGAAATTTAAAGCATCTAGAGATAGCATATTATCAGAAATGAAG
  ATGCTAAAAGAGAAGAGGCAGCAGTCAGAGAAAACCTTCATGCCTAAGCAACGTAGCTTA
  CAGAGTTTGGAGGCAAGCTTGCATGCTATGGAGTCTACCAGAGAGTCATTGAAAGCAGAA
  CTGGGAACTGATTTGCTTTCTCAACTGAGTTTGGAAGATCAGAAGAGAGTAGATGCACTG
  AATGATGAGATTCGTCAACTTCAGCAGGAAAACAGACAGTTGCTAAATGAAAGAATTAAA
  TTAGAAGGTATTATTACTCGAGTAGAGACTTATCTCAATGAGAATCTGAGAAAACGCTTG
  GACCAAGTAGAACAGGAACTTAATGAGCTGAGAGAGACAGAAGGGGGTACTGTTCTCACA
  GCCACAACATCAGAACTTGAAGCCATCAATAAAAGAGTAAAAGACACTATGGCACGATCA
  GAAGATTTGGACAATTCCATTGATAAAACAGAAGCTGGAATTAAGGAGCTTCAGAAGAGT
  ATGGAGCGCTGGAAAAATATGGAAAAAGAACATATGGATGCTATAAATCATGATACTAAA
  GAACTGGAAAAGATGACAAATCGGCAAGGCATGCTATTGAAGAAGAAAGAAGAGTGTATG
  AAGAAAATTCGAGAACTTGGATCACTTCCCCAGGAAGCATTTGAAAAGTACCAGACACTG
  AGCCTCAAACAGTTGTTTCGAAAACTTGAGCAGTGCAACACAGAATTAAAGAAGTACAGC
  CATGTTAACAAAAAGGCTTTGGATCAGTTTGTAAATTTCTCCGAGCAGAAAGAAAAGTTA
  ATAAAGCGTCAAGAAGAGTTAGATAGGGGTTACAAATCGATCATGGAACTGATGAATGTA
  CTTGAACTTCGGAAATATGAAGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTC
  AGTGAAGTATTCCAGAAGTTAGTACCTGGTGGCAAAGCTACTTTGGTGATGAAGAAAGGA
  GATGTGGAGGGCAGTCAGTCTCAAGATGAAGGAGAAGGGAGTGGTGAGAGTGAGAGGGGT
  TCTGGCTCACAAAGCAGTGTCCCATCAGTTGACCAGTTTACTGGAGTTGGAATTAGGGTG
  TCATTTACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAGGTGGACAGAAA
  TCCTTGGTAGCCCTTGCTCTGATTTTTGCCATTCAGAAATGTGACCCGGCTCCATTTTAC
  TTGTTTGATGAAATTGACCAGGCTCTGGATGCTCAGCACAGAAAGGCTGTGTCAGATATG
  ATTATGGAACTTGCTGTACATGCTCAGTTTATTACAACTACTTTTAGGCCTGAACTGCTT
  GAGTCAGCTGACAAATTCTATGGTGTAAAGTTCAGAAATAAGGTTAGTCATATTGATGTG
  ATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGATGATACCACACATGGTTAA
  
  Clone variation with respect to NM_005445.3
  3039 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005445 unedited
  TGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGGAGGGGTCGCGTAG
  GCGCCTCACCTGACCCTGCGGCCGTGCGGTTGCTGCTCCGGGGCAGGTCTCCTTCCAGGC
  CAGGGGCCCGGAATCATGTACATAAAGCAGGTGATTATCCAGGGTTTTCGAAGTTACAGA
  GATCAAACAATTGTAGATCCCTTCAGTTCAAAACATAATGTGATTGTGGGCAGAAATGGA
  TCTGGAAAAAGTAACTTTTTTATGCAATTCAGTTTGTTCTCAGTGATGAGTTTAGTCATC
  TTCGTCCAGAACAGCGGTTGGCTTTATTGCATGAAGGTACTGGTCCTCGTGTTATTTCTG
  CTTTTGTGGAGATTATTTTTGATAATTCAGACAACCGGTTACCAATCGATAAAGAGGAAG
  TTTCACTTCGAAGAGTTATTGGTGCCAAAAAGGATCAGTATTTCTTAGACAAGAAGATGG
  TCACGAAAAATGATGTGATGAACCTCCTTGAAAGCGCTGGTTTTTCTCGAAGCAATCCTT
  ATTATATTGTTAAACAAGGAAAGATCAACCAGATGGCAACAGCACCAGATTCTCAGAGAT
  TAAAGCTATTAAGAGAAGTAGCTGGTACTAGAGTGTATGACGAACGAAAGGAAGAAAGCA
  TCTCCTTAATGAAAGAAACAGAGGGCAAACGGGAAAAATCAATGGAGTTGTTAAAATACA
  TTGAAGAGAGATTACATACTCTAGAGGAAGAAANGAAGAACTAGCTCAGTTNCAGAAGTG
  GGATANAATGAGACGAGCCCTGGNATATACCATTTACAATCAGAACTTACGAGACTCGTG
  CCAACTTGATGAGCTTTCTGCTAGCGAGAGACTAGTGGAGAANAATCCAGACATTAGAGA
  TGCTCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005445 unedited
  GCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAGTCATAAAACCGATTATT
  TAATTGAAGCTATAAAAAAGGTAGTATAAGTGATAAAATAATTAGGAAAGAATATTTAGC
  ATGTTTCAAAACATTTAAAATAGGAGCAGAACATTTTACAAAAAGTTGTACAGGAAATTA
  AATTCTTAAACTATCAGTACAAACATGACATTACAGAGTATCTTATAAAATACAAAGACA
  AATATAAAAGGACTATGATGCTTTAAGTCTGAAAACTATTGGCCAAATATTTAGGTTTAA
  ATTTACAGTTCCTGGGTATGAGAATCATATTACTATATACATCTCCCAAACCAGTAGGTA
  GTATTTTCCAATTAACCATGTGTGGTATCATCTTCTACAAAGTCTTTGGCCATCTCTGCT
  GTGATCACATCAATATGACTAACCTTATTTCTGAACTTTACACCATAGAATTTGTCAGCT
  GACTCAAGCAGTTCAGGCCTAAAAGTAGTTGTAATAAACTGAGCATGTACAGCAAGTTCC
  ATAATCATATCTGACACAGCCTTTCTGTGCTGAGCATCCAGAGCCTGGTCAATTTCATCA
  AACAAGTAAAATGGAGCCGGGTCACATTTCTGAATGGCANAAATCAGAGCAAGGGCTACC
  AAGGATTTCTGTCCACCTGAAAAGCTGTGCATTNCTCTCATTTCACCTTGTTTTCCTGGA
  AATGACACCCTAATTCCAACTCCAGTAAACTGTTCAACTGATGGGACACTGCTTTGTGAG
  CCANAACCCCTNTNNACTTTACCACTNCCTTTNTNCTTNATCTTGAAGACTGACTGGCCC
  TCCACATTTCCTTTCTTTATCACCAAAGTAGCTTTGCCACCAGGTACTAACTTCTGGGAT
  ACTTCACTTGAGTTCCTANATAACTGGTTTGAAGNTTACTGGAATACCTCATATTTCCGA
  GGTTCAGGACCTTCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005445
• ORF Size: 3654 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_005445.3, NP_005436.1
• RefSeq Size: 4131
• RefSeq ORF: 3654
• Locus ID: 9126
• Domains: SMC_N, SMC_C
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis
• Gene Summary: This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]