EXOSC6 (NM_058219) Human Tagged ORF Clone

CAT#: RC208634

EXOSC6 (Myc-DDK-tagged)-Human exosome component 6 (EXOSC6)

Product Images

Western blot validation of overexpression lysate (Cat# LY409238) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208634 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOSC6
• Synonyms: EAP4; hMtr3p; MTR3; Mtr3p; p11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208634 representing NM_058219
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGGATCACCGCCGCATCCGCGGCCCTGAAGAATCGCAGCCGCCGCAGCTGTACGCGGCCGACG
  AGGAGGAGGCGCCCGGCACCCGCGACCCAACGCGGCTACGGCCCGTGTACGCGCGCGCCGGGCTGCTGAG
  CCAGGCCAAGGGCTCGGCCTACCTGGAGGCGGGAGGCACCAAGGTGCTGTGTGCCGTGTCGGGCCCGCGA
  CAGGCCGAGGGCGGCGAGCGCGGCGGCGGCCCGGCCGGAGCAGGCGGCGAGGCCCCGGCCGCGCTGCGCG
  GTCGCCTGCTCTGCGACTTCCGCCGCGCACCCTTCGCGGGCCGCCGGCGCCGCGCTCCCCCGGGCGGCTG
  CGAGGAGCGTGAGCTGGCGCTGGCGCTGCAGGAGGCGCTGGAGCCGGCTGTGCGCCTGGGCCGCTACCCG
  CGCGCGCAGCTCGAGGTGTCGGCGCTGCTGCTGGAGGACGGTGGCTCGGCCCTGGCCGCCGCGCTCACCG
  CCGCCGCGCTCGCCCTGGCCGACGCGGGCGTGGAGATGTACGACCTGGTGGTGGGCTGCGGCCTCAGCCT
  CGCGCCGGGGCCCGCGCCCACCTGGCTCCTGGACCCCACGCGGCTCGAGGAAGAGCGCGCCGCCGCCGGC
  CTCACCGTGGCGCTCATGCCTGTGCTGAATCAGGTGGCCGGGCTGCTGGGCAGCGGCGAGGGCGGCCTGA
  CAGAGAGCTGGGCGGAGGCCGTACGCCTGGGCCTCGAGGGCTGCCAGCGCCTCTACCCCGTGCTGCAGCA
  GAGCCTGGTGCGGGCCGCCCGCCGCAGGGGCGCCGCCGCCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208634 representing NM_058219
  Red=Cloning site Green=Tags(s)
  
  MPGDHRRIRGPEESQPPQLYAADEEEAPGTRDPTRLRPVYARAGLLSQAKGSAYLEAGGTKVLCAVSGPR
  QAEGGERGGGPAGAGGEAPAALRGRLLCDFRRAPFAGRRRRAPPGGCEERELALALQEALEPAVRLGRYP
  RAQLEVSALLLEDGGSALAAALTAAALALADAGVEMYDLVVGCGLSLAPGPAPTWLLDPTRLEEERAAAG
  LTVALMPVLNQVAGLLGSGEGGLTESWAEAVRLGLEGCQRLYPVLQQSLVRAARRRGAAAQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058219
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_058219.1, NM_058219.2, NP_478126.1
• RefSeq Size: 1729
• RefSeq ORF: 819
• Locus ID: 118460
• Domains: RNase_PH_C
• Protein Pathways: RNA degradation
• MW: 28.2 kDa
• Gene Summary: This gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation. [provided by RefSeq, Jul 2008]