SOX4 (NM_003107) Human Tagged ORF Clone

CAT#: RC209139

SOX4 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 4 (SOX4)

Product Images

Western blot validation of overexpression lysate (Cat# LY418894) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SOX4 protein (Cat# TP309139). The protein was produced from HEK293T cells transfected with SOX4 cDNA clone (Cat# RC209139) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOX4
• Synonyms: CSS10; EVI16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209139 representing NM_003107.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGCAGCAAACCAACAATGCCGAGAACACGGAAGCGCTGCTGGCCGGCGAGAGCTCGGACTCGGGC
  GCCGGCCTCGAGCTGGGAATCGCCTCCTCCCCCACGCCCGGCTCCACCGCCTCCACGGGCGGCAAGGCC
  GACGACCCGAGCTGGTGCAAGACCCCGAGTGGGCACATCAAGCGACCCATGAACGCCTTCATGGTGTGG
  TCGCAGATCGAGCGGCGCAAGATCATGGAGCAGTCGCCCGACATGCACAACGCCGAGATCTCCAAGCGG
  CTGGGCAAACGCTGGAAGCTGCTCAAAGACAGCGACAAGATCCCTTTCATTCGAGAGGCGGAGCGGCTG
  CGCCTCAAGCACATGGCTGACTACCCCGACTACAAGTACCGGCCCAGGAAGAAGGTGAAGTCCGGCAAC
  GCCAACTCCAGCTCCTCGGCCGCCGCCTCCTCCAAGCCGGGGGAGAAGGGAGACAAGGTCGGTGGCAGT
  GGCGGGGGCGGCCATGGGGGCGGCGGCGGCGGCGGGAGCAGCAACGCGGGGGGAGGAGGCGGCGGTGCG
  AGTGGCGGCGGCGCCAACTCCAAACCGGCGCAGAAAAAGAGCTGCGGCTCCAAAGTGGCGGGCGGCGCG
  GGCGGTGGGGTTAGCAAACCGCACGCCAAGCTCATCCTGGCAGGCGGCGGCGGCGGCGGGAAAGCAGCG
  GCTGCCGCCGCCGCCTCCTTCGCCGCCGAACAGGCGGGGGCCGCCGCCCTGCTGCCCCTGGGCGCCGCC
  GCCGACCACCACTCGCTGTACAAGGCGCGGACTCCCAGCGCCTCGGCCTCCGCCTCCTCGGCAGCCTCG
  GCCTCCGCAGCGCTCGCGGCCCCGGGCAAGCACCTGGCGGAGAAGAAGGTGAAGCGCGTCTACCTGTTC
  GGCGGCCTGGGCACGTCGTCGTCGCCCGTGGGCGGCGTGGGCGCGGGAGCCGACCCCAGCGACCCCCTG
  GGCCTGTACGAGGAGGAGGGCGCGGGCTGCTCGCCCGACGCGCCCAGCCTGAGCGGCCGCAGCAGCGCC
  GCCTCGTCCCCCGCCGCCGGCCGCTCGCCCGCCGACCACCGCGGCTACGCCAGCCTGCGCGCCGCCTCG
  CCCGCCCCGTCCAGCGCGCCCTCGCACGCGTCCTCCTCGGCCTCGTCCCACTCCTCCTCTTCCTCCTCC
  TCGGGCTCCTCGTCCTCCGACGACGAGTTCGAAGACGACCTGCTCGACCTGAACCCCAGCTCAAACTTT
  GAGAGCATGTCCCTGGGCAGCTTCAGTTCGTCGTCGGCGCTCGACCGGGACCTGGATTTTAACTTCGAG
  CCCGGCTCCGGCTCGCACTTCGAGTTCCCGGACTACTGCACGCCCGAGGTGAGCGAGATGATCTCGGGA
  GACTGGCTCGAGTCCAGCATCTCCAACCTGGTTTTCACCTAC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC209139
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVW
  SQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGN
  ANSSSSAAASSKPGEKGDKVGGSGGGGHGGGGGGGSSNAGGGGGGASGGGANSKPAQKKSCGSKVAGGA
  GGGVSKPHAKLILAGGGGGGKAAAAAAASFAAEQAGAAALLPLGAAADHHSLYKARTPSASASASSAAS
  ASAALAAPGKHLAEKKVKRVYLFGGLGTSSSPVGGVGAGADPSDPLGLYEEEGAGCSPDAPSLSGRSSA
  ASSPAAGRSPADHRGYASLRAASPAPSSAPSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNF
  ESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYCTPEVSEMISGDWLESSISNLVFTY
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC209139 also available, TP309139M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003107
• ORF Size: 1422 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003107.3
• RefSeq Size: 4912 bp
• RefSeq ORF: 1425 bp
• Locus ID: 6659
• UniProt ID: Q06945
• Cytogenetics: 6p22.3
• Domains: HMG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• MW: 47.3 kDa
• Gene Summary: This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]