AGO1 (NM_012199) Human Tagged ORF Clone
CAT#: RC209163L4
- LentiORF®
Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), mGFP tagged
"NM_012199" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | AGO1 |
Synonyms | EIF2C; EIF2C1; GERP95; hAgo1; Q99 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC209163).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_012199 |
ORF Size | 2571 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012199.2 |
RefSeq Size | 7478 bp |
RefSeq ORF | 2574 bp |
Locus ID | 26523 |
UniProt ID | Q9UL18 |
Cytogenetics | 1p34.3 |
Protein Families | Druggable Genome |
MW | 97 kDa |
Gene Summary | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303941 | AGO1 (untagged)-Human eukaryotic translation initiation factor 2C, 1 (EIF2C1) |
USD 760.00 |
|
RC209163 | AGO1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 1 (EIF2C1) |
USD 420.00 |
|
RG209163 | AGO1 (GFP-tagged) - Human eukaryotic translation initiation factor 2C, 1 (EIF2C1) |
USD 460.00 |
|
RC209163L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), Myc-DDK-tagged |
USD 768.00 |
|
RC209163L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), mGFP tagged |
USD 620.00 |
|
RC209163L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 1 (EIF2C1), Myc-DDK-tagged |
USD 620.00 |
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