AGO1 (NM_012199) Human Untagged Clone

CAT#: SC303941

AGO1 (untagged)-Human eukaryotic translation initiation factor 2C, 1 (EIF2C1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AGO1
• Synonyms: EIF2C; EIF2C1; GERP95; hAgo1; Q99
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012199 edited
  GGTACCTAGGCCCCTCACGCTGGACTTCACAGTCTCCGGGCCGCCTGACCTCCGCACGGG
  TATATGGGATGGAAGCGGGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGC
  AGCAGGTGTTCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATCAAGC
  TCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGTACCACTACGAGGTGG
  ACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACCGGGAAGTGGTGGAATACATGGTCC
  AGCATTTCAAGCCTCAGATCTTTGGTGATCGCAAGCCTGTGTATGATGGAAAGAAGAACA
  TTTACACTGTCACAGCACTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCC
  CTGGGGAAGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCT
  GGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCCCTTGGAGTCTGTGC
  AAGCCCTGGATGTGGCCATGAGGCACCTGGCATCCATGAGGTACACCCCTGTGGGCCGCT
  CCTTCTTCTCACCGCCTGAGGGCTACTACCACCCGCTGGGGGGTGGGCGCGAGGTCTGGT
  TCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCT
  CAGCCACTGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCTGGACA
  TCAGGAACATAAATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCGCGTTCGCTTCACCA
  AGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACTGTGGACAGATGAAGAGGAAGTACC
  GCGTGTGTAATGTTACCCGTCGCCCTGCTAGCCATCAGACATTCCCCTTACAGCTGGAGA
  GTGGACAGACTGTGGAGTGCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGC
  TCAAGTATCCCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTACCTTC
  CCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAAAGCTGACCGACAACC
  AGACCTCGACCATGATAAAGGCCACAGCTAGATCCGCTCCAGACAGACAGGAGGAGATCA
  GTCGCCTGATGAAGAATGCCAGCTACAACTTAGATCCCTACATCCAGGAATTTGGGATCA
  AAGTGAAGGATGACATGACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGT
  ACGGCGGCCGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGGGGGA
  AACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAA
  AACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACAGACCAGCTGCGGAAGATTTCCAAGG
  ATGCGGGGATGCCTATCCAGGGTCAACCTTGTTTCTGCAAATATGCACAGGGGGCAGACA
  GCGTGGAGCCTATGTTCCGGCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTG
  TCATCCTGCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACACTCT
  TGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGACCTCACCTCAGACTC
  TGTCCAACCTCTGCCTCAAGATCAATGTCAAACTTGGTGGCATTAACAACATCCTAGTCC
  CACACCAGCGCTCTGCCGTTTTTCAACAGCCAGTGATATTCCTGGGAGCAGATGTTACAC
  ACCCCCCAGCAGGGGATGGGAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATG
  CCCACCCCAGCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGATCATTG
  AAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAAGTCCACCCGTTTCA
  AGCCTACCCGCATCATCTTCTACCGAGATGGGGTGCCTGAAGGCCAGCTACCCCAGATAC
  TCCACTATGAGCTACTGGCCATTCGTGATGCCTGCATCAAACTGGAAAAGGACTACCAGC
  CTGGGATCACTTATATTGTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACA
  AGAATGAGCGAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACCAACA
  TCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAGGCATCCAGGGCACCA
  GCCGACCATCCCATTACTATGTTCTTTGGGATGACAACCGTTTCACAGCAGATGAGCTCC
  AGATCCTGACGTACCAGCTGTGCCACACTTACGTACGATGCACACGCTCTGTCTCTATCC
  CAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACA
  AGGAGCATGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGACCCCC
  AGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCACCATGTACTTCGCTT
  GAAGGCAGAACGCTGTTACCTCACTGGATAGAAGAAAGCTTTCCAAGCCCCAGGAGCTGT
  GCCACCCAAATCCAGAGGAAGCAAGGAGGAGGGAGGTGGGGTAGGGAGGAGTGTAGGATG
  CCTTGTTTCCTTCTATAGAGGTGGTGTAAGAGTGGGGAACAGGGCCAGCAAGACAGACCA
  CCAGCCAGAAATCTCTGATATCAACCTCATGTCCCCCACCCCTCACCCCATCTTGTCACA
  TCTGGCCCTGACCCCACTGGACCAAAAGGGGCAGCACTGGTGCCCACCATACACACAGGT
  GTCTCATGTGACTCACAGTGCTAAAGACTCATGCTTGACAGCTTGGTAAGGTCAACTCTG
  TAGCCCTGCAGACAAAAGCTGGTTAGGTTTGGGTTTGATACTTTAGATGGGAAAGTGAGG
  GGCTTGAGAAAGTGGGTGGGAGGAGGGAAGGATTTTTTAGGAGCCTTAATCAGAAAAGGA
  CTAGATTTGTTTAAGAAGAAAAATGAAACCAGACCCAGATCAATATTTTAGGATACTAGA
  TGTTTTAATGGGTTCAGAATCCAGTTTGTAGGAAGATTTTTTAATGGTTTTGGTTGCTCC
  TCCCCCAGCTGCCACCCCCCACCTTACCCTTATTCCTCTCTGTCCACATTTTCTGCCCCA
  CCTTACTTCTCCTCCCTGACAGACATCCAGCCCCTAGTAATACTTAAGGCACTATGGCAC
  TTAGCTTTGAAGTGACACGACCCTGTCTTCCTTCCGCCCGCTGGTGGGTAACCAGTGCCT
  TCCCTGTAACGGTAATGCTGCAGAACTGCAACCTTTTGTACCTTTCTTTGGGGAATGGGG
  TGGGGGTGGGAGAGGAGGTAGATGGGGAAGAAATACCCCAGACCCAACAAACCTCCAGCC
  AGAAAGCCAGCTATTTTGCATTTGAAGGAATTGACTTCCTCATTCATTGAGCTTTTTAAA
  AGATCACAACCTCAAGATGGTTAAAATCCATTGACATTTGCACTTTCAAACATGACAAGT
  CTCGGAGCTGCTGAGATGACAGGCCCCTGGCCTTTCCACTTATGCCTCCTTTTCTCCTTA
  TTCCTCCTACCTCCCGCCCCGCCCAGGTCTGGAGTTACTTTCATAGCATTTTTCACTCTT
  GGCTTCTTTTCTCCCTTGATGGTCAAGTCTCTTATGTTTCAATATTTCTTAACTGGGGTG
  TCTTATAACAAAAAACTCTTAGGTCTAAAATGAGAAAAAAGAGAGAAAACAAAATGTTAT
  TTTTATACCATAACTTGAGTGTATTGCCAAAATTTGGAAATCCTTCCCATGCCTGATGAG
  TTTATATCCCAGAAACATTGAGCCATCAGAACGAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_012199
• ORF Size: 2574 bp
• Insert Size: 4000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: ORF matches with NM_012199.2.

Reference Data

• RefSeq: NM_012199.2, NP_036331.1
• RefSeq Size: 7478
• RefSeq ORF: 2574
• Locus ID: 26523
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1).