PPM1F (NM_014634) Human Tagged ORF Clone

CAT#: RC210043

PPM1F (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F)

Product Images

Western blot validation of overexpression lysate (Cat# LY402357) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210043 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPM1F protein (Cat# TP310043). The protein was produced from HEK293T cells transfected with PPM1F cDNA clone (Cat# RC210043) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPM1F
• Synonyms: CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210043 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCAGGCTTCCTGG
  ACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTGCCATGGAAGGCCCCAGGGAC
  GGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAGCTGGCCATGGGCTTTCTGGGCAGCAGGAAG
  GCCCCGCCACCACTTGCTGCTGCTCTGGCCCACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCG
  AATTCAGGAAGTTGCCCAGGGAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAAAAGGCCCCTGTGAC
  CTTGCTGGATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGGCAG
  AAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCACGCCATCCGGAACA
  CTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAACCAGCTCTTCGGCTTGTCTGACCC
  TGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCACGGAGGCGTGGATGCTGCGAGGTACGCCGCTGTC
  CACGTGCACACCAACGCTGCCCGCCAGCCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAGCCT
  TCCGGCGCACCGACCAGATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCAGAGCGGCACCACAGGTGT
  GTGTGCGCTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTACAG
  CAGGGACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAGAAGGCGCGCATTGAAG
  CATTGGGTGGCTTTGTGTCTCACATGGACTGCTGGAGAGTCAACGGGACCCTGGCCGTCTCCAGAGCCAT
  CGGGGATGTCTTCCAGAAGCCCTACGTGTCTGGGGAGGCCGATGCAGCTTCCCGGGCGCTGACGGGCTCC
  GAGGACTACCTGCTGCTTGCCTGTGATGGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGTTGGCCTGG
  TCCAGAGCCACCTGACCAGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCG
  GGAGCGGGGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCCCCAAGAGCTGCTGGAG
  GGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAGAAGGGAGGAGGCAGGACTTGCCCTCCAGCCTTCCAG
  AACCTGAGACCCAGGCTCCACCAAGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210043 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSGAPQKSSPMASGAEETPGFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRK
  APPPLAAALAHEAVSQLLQTDLSEFRKLPREEEEEEEDDDEEKAPVTLLDAQSLAQSFFNRLWEVAGQWQ
  KQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLPSFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAV
  HVHTNAARQPELPTDPEGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQVILVQ
  QGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRALTGS
  EDYLLLACDGFFDVVPHQEVVGLVQSHLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELLE
  GGNQGEGDPQAEGRRQDLPSSLPEPETQAPPRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014634
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014634.1, NM_014634.2, NM_014634.3, NP_055449.1
• RefSeq Size: 5199 bp
• RefSeq ORF: 1365 bp
• Locus ID: 9647
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• MW: 49.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]