PPM1F (NM_014634) Human Untagged Clone

CAT#: SC114924

PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1F
• Synonyms: CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114924 sequence for NM_014634 edited (data generated by NextGen Sequencing)
  ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA
  GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG
  CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG
  CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC
  CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG
  GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG
  GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG
  CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC
  GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC
  CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC
  GGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG
  CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAGCCTTCCGGCGCACCGACCAG
  ATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCAGAGCGGCACCACAGGTGTGTGTGCG
  CTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTA
  CAGCAGGGACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAGAAG
  GCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCACATGGACTGCTGGAGAGTCAACGGG
  ACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTTCCAGAAGCCCTACGTGTCTGGGGAG
  GCCGATGCAGCTTCCCGGGCGCTGACGGGCTCCGAGGACTACCTGCTGCTTGCCTGTGAT
  GGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGTTGGCCTGGTCCAGAGCCACCTGACC
  AGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCGGGAGCGG
  GGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCCCCAAGAGCTGCTG
  GAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAGAAGGGAGGAGGCAGGACTTGCCC
  TCCAGCCTTCCAGAACCTGAGACCCAGGCTCCACCAAGAAGCTAG
  
  Clone variation with respect to NM_014634.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014634 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCGCGGAGACCGGG
  GGTGAAGCCTGGAGACCCTCTTGCCCTGGCCTAGCTGCAGGCCCCCGGGATGCTTTGGGC
  ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA
  GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG
  CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG
  CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC
  CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG
  GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG
  GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG
  CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC
  GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC
  CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC
  GGNAGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG
  CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAACCTTCCGGCGCACCGACCAG
  ATGNNTCTCAGGGAAGCCAAGCGAGAGCGGCTGCAGAGCGCACCACAGTGGTGTGTGCGC
  TCATTGGCAGAGCGACCNTGCACGTCGCCTGGCTCGGGGATTCCCAGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014634 unedited
  CCGCGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCTTGAAAAAAGCTTTAA
  AAAAGTAAATACTTAAGTTTTCTAAAGAATAAAATAAATGCTAAGCTCTGCTTAAATTAT
  ATGACACAACAGGTCATCTTGGCACTGATAAAACAAAGAGAAAACCTGGAATACTGCTTC
  GGAATTAGACCTCCCTGGAAAAAAACAAACACACCAACAAAAGACACATGTGCGTCGCCG
  GTTCCGGGCTCAGGGAAGAGAGCAGCCCGGAAGGCCCTGGTGGGCCCAAGCACATCATGG
  CTGCCCTGGAGTGGGGGCAGGGGGGTGGCTGCTACAGAACAGACCCCTGGCTCTGCTGCG
  CTCTCCTCTTTGGGACACTGGTGTGGCCGACCAGGACTGGCTGGGCATATCTGTAAATAT
  GAACCCACCTGGCCCTGGCCGCAGAGAGGAGCAATGGGAAACTGGGGCTGGCCACTCCGC
  TGCTGGGCCTGACCCAGGGGGTGCTTCAAGAGTTTGGCTCCTGGGTCCGCCCCAGGGTTC
  CACGTGCACAACCATGCTGCCCCATGCACACCAGCGTCTTTGGTTTGGCTGCCGTTGCGC
  ACCTATGACCTCTGGACCCACCCCGGGCCTACTAGGACCTGGGAGCAAGAGCCCCTCCAT
  CTTTTCTTTGGCGAGGCCTCCTAACCTGCCTTCCCCCTCCTGCCGCCACCAGTGCAGTTC
  ACAGCCACAAGAAGGCCTGTGGGGGGTGCTCCAACCGCCTTGGGGCGCTTGGGAAACCCT
  GCGGGGCGGGCCCCTGTCCACTGCCTGCACCTGTGGCCCCGAGGCCTCTGAGGAACACGC
  CACCGATGCAGCATGTCAGCCCCCCCCCTTCACCTTTTACCCTTGTTTTACCCCCCTTCA
  CTATTCAAATCCCTTCCTCCCCTCTTCTATCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014634
• ORF Size: 1365 bp
• Insert Size: 2440
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_014634.2, NP_055449.1
• RefSeq Size: 5169
• RefSeq ORF: 1365
• Locus ID: 9647
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]