PLRG1 (NM_002669) Human Tagged ORF Clone

CAT#: RC210603

PLRG1 (Myc-DDK-tagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210603 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLRG1
• Synonyms: Cwc1; PRL1; PRP46; PRPF46; TANGO4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210603 representing NM_002669
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGAGGAGGTACAGAAACATTCTGTACACACCCTTGTGTTCAGGTCGTTGAAGAGGACCCATGACA
  TGTTTGTAGCTGATAATGGAAAACCTGTGCCTTTAGATGAAGAGAGTCACAAACGAAAAATGGCAATCAA
  GCTTCGTAATGAGTATGGTCCTGTGTTGCATATGCCTACTTCAAAAGAAAATCTTAAAGAGAAGGGTCCT
  CAGAATGCAACGGATTCATATGTTCATAAACAGTACCCTGCCAATCAAGGACAAGAAGTTGAATACTTTG
  TGGCAGGTACACATCCATACCCACCAGGACCTGGGGTTGCTTTGACAGCAGATACTAAGATCCAGAGAAT
  GCCAAGTGAATCAGCTGCACAGTCCTTAGCGGTGGCATTACCTTTGCAGACCAAGGCTGATGCAAATCGT
  ACTGCCCCTAGTGGAAGTGAATACCGACATCCTGGGGCTTCTGACCGTCCACAGCCTACAGCGATGAATT
  CAATTGTCATGGAGACTGGCAATACCAAGAACTCTGCACTGATGGCTAAAAAAGCCCCTACAATGCCAAA
  ACCCCAGTGGCACCCACCGTGGAAACTCTACAGGGTTATCAGTGGGCATCTTGGCTGGGTTCGATGTATT
  GCTGTGGAACCTGGAAATCAGTGGTTTGTTACTGGATCTGCTGACAGAACTATAAAGATCTGGGACTTGG
  CTAGTGGCAAATTAAAACTGTCATTGACTGGGCATATTAGTACTGTGCGGGGCGTGATAGTAAGCACAAG
  GAGCCCATATCTGTTCTCTTGTGGAGAAGACAAACAAGTGAAATGCTGGGATCTCGAATACAATAAGGTT
  ATACGGCATTATCATGGACATTTAAGTGCAGTGTATGGTTTGGATTTGCACCCGACAATCGATGTGTTGG
  TAACCTGTAGTCGAGATTCAACTGCACGGATTTGGGATGTGAGAACTAAAGCCAGTGTACACACATTATC
  TGGACATACAAATGCAGTTGCTACAGTGAGATGTCAGGCTGCAGAACCACAAATTATTACAGGAAGCCAT
  GATACTACAATTCGATTATGGGATCTGGTGGCTGGAAAAACAAGAGTGACATTAACAAATCACAAAAAAT
  CAGTTAGGGCTGTGGTTTTACATCCAAGACATTACACATTTGCATCTGGTTCTCCAGATAACATAAAGCA
  GTGGAAATTCCCTGATGGAAGTTTCATTCAAAATCTTTCCGGTCATAATGCTATTATTAACACATTGACG
  GTAAATTCTGATGGAGTGCTTGTATCTGGAGCTGACAATGGCACCATGCATCTTTGGGACTGGAGAACTG
  GCTACAATTTTCAGAGAGTTCACGCAGCTGTGCAACCTGGGTCTTTGGACAGTGAATCAGGAATATTTGC
  TTGTGCTTTTGATCAGTCTGAAAGTCGATTACTAACAGCTGAAGCTGATAAAACCATTAAAGTATACAGA
  GAGGATGACACAGCCACAGAAGAAACTCATCCAGTCAGCTGGAAACCAGAAATTATCAAGAGAAAGAGAT
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210603 representing NM_002669
  Red=Cloning site Green=Tags(s)
  
  MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPTSKENLKEKGP
  QNATDSYVHKQYPANQGQEVEYFVAGTHPYPPGPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANR
  TAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCI
  AVEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKV
  IRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSH
  DTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLT
  VNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYR
  EDDTATEETHPVSWKPEIIKRKRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002669
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002669.1, NM_002669.2, NM_002669.3, NP_002660.1
• RefSeq Size: 3374 bp
• RefSeq ORF: 1545 bp
• Locus ID: 5356
• Cytogenetics: 4q31.3
• Domains: WD40
• Protein Families: Transcription Factors
• Protein Pathways: Spliceosome
• MW: 57.2 kDa
• Gene Summary: 'This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]'