PLRG1 (NM_002669) Human Untagged Clone

CAT#: SC118503

PLRG1 (untagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLRG1
• Synonyms: Cwc1; PRL1; PRP46; PRPF46; TANGO4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118503 sequence for NM_002669 edited (data generated by NextGen Sequencing)
  ATGGTCGAGGAGGTACAGAAACATTCTGTACACACCCTTGTGTTCAGGTCGTTGAAGAGG
  ACCCATGACATGTTTGTAGCTGATAATGGAAAACCTGTGCCTTTAGATGAAGAGAGTCAC
  AAACGAAAAATGGCAATCAAGCTTCGTAATGAGTATGGTCCTGTGTTGCATATGCCTACT
  TCAAAAGAAAATCTTAAAGAGAAGGGTCCTCAGAATGCAACGGATTCATATGTTCATAAA
  CAGTACCCTGCCAATCAAGGACAAGAAGTTGAATACTTTGTGGCAGGTACACATCCATAC
  CCACCAGGACCTGGGGTTGCTTTGACAGCAGATACTAAGATCCAGAGAATGCCAAGTGAA
  TCAGCTGCACAGTCCTTAGCGGTGGCATTACCTTTGCAGACCAAGGCTGATGCAAATCGT
  ACTGCCCCTAGTGGAAGTGAATACCGACATCCTGGGGCTTCTGACCGTCCACAGCCTACA
  GCGATGAATTCAATTGTCATGGAGACTGGCAATACCAAGAACTCTGCACTGATGGCTAAA
  AAAGCCCCTACAATGCCAAAACCCCAGTGGCACCCACCGTGGAAACTCTACAGGGTTATC
  AGTGGGCATCTTGGCTGGGTTCGATGTATTGCTGTGGAACCTGGAAATCAGTGGTTTGTT
  ACTGGATCTGCTGACAGAACTATAAAGATCTGGGACTTGGCTAGTGGCAAATTAAAACTG
  TCATTGACTGGGCATATTAGTACTGTGCGGGGCGTGATAGTAAGCACAAGGAGCCCATAT
  CTGTTCTCTTGTGGAGAAGACAAACAAGTGAAATGCTGGGATCTCGAATACAATAAGGTT
  ATACGGCATTATCATGGACATTTAAGTGCAGTGTATGGTTTGGATTTGCACCCGACAATC
  GATGTGTTGGTAACCTGTAGTCGAGATTCAACTGCACGGATTTGGGATGTGAGAACTAAA
  GCCAGTGTACACACATTATCTGGACATACAAATGCAGTTGCTACAGTGAGATGTCAGGCT
  GCAGAACCACAGATTATTACAGGAAGCCATGATACTACAATTCGATTATGGGATCTGGTG
  GCTGGAAAAACAAGAGTGACATTAACAAATCACAAAAAATCAGTTAGGGCTGTGGTTTTA
  CATCCAAGACATTACACATTTGCATCTGGTTCTCCAGATAACATAAAGCAGTGGAAATTC
  CCTGATGGAAGTTTCATACAAAATCTTTCCGGTCATAATGCTATTATTAACACATTGACG
  GTAAATTCTGATGGAGTGCTTGTATCTGGAGCTGACAATGGCACCATGCATCTTTGGGAC
  TGGAGAACTGGCTACAATTTTCAGAGAGTTCACGCAGCTGTGCAACCTGGGTCTTTGGAC
  AGTGAATCAGGAATATTTGCTTGTGCTTTTGATCAGTCTGAAAGTCGATTACTAACAGCT
  GAAGCTGATAAAACCATTAAAGTATACAGAGAGGATGACACAGCCACAGAAGAAACTCAT
  CCAGTCAGCTGGAAACCAGAAATTATCAAGAGAAAGAGATTTTAA
  
  Clone variation with respect to NM_002669.3
  1032 a=>g;1218 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002669 unedited
  TATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCACAGCTGTGG
  CGGCGGGTACTGCGTTAGTGATTAGAGTTTCTTCCCTGCCGGAGGTGGGATACACGGTAG
  CATCATGGTCGAGGAGGTACAGAAACATTCTGTACACACCCTTGTGTTCAGGTCGTTGAA
  GAGGACCCATGACATGTTTGTAGCTGATAATGGAAAACCTGTGCCTTTAGATGAAGAGAG
  TCACAAACGAAAAATGGCAATCAAGCTTCGTAATGAGTATGGTCCTGTGTTGCATATGCC
  TACTTCAAAAGAAAATCTTAAAGAGAAGGGTCCTCAGAATGCAACGGATTCATATGTTCA
  TAAACAGTACCCTGCCAATCAAGGACAAGAAGTTGAATACTTTGTGGCAGGTACACATCC
  ATACCCACCAGGACCTGGGGTTGCTTTGACAGCAGATACTAAGATCCAGAGAATGCCAAG
  TGAATCAGCTGCACAGTCCTTAGCGGTGGCATTACCTTTGCAGACCAAGGCTGATGCAAA
  TCGTACTGCCCCTAGTGGAAGTGAATACCGACATCCTGNGGCTTCTGACCGTCCACAGCC
  TACAGCGATGAATTCAATTGTCATGGAGACTGGCAATACCAAGAACTCTGCACTGATGGC
  TAAAAAAGCCCCTACAATGCCAAAACCCCAGTGGCACCCACCGTGGAAACTCTACAGGGT
  TATCAGTGGGCATCTTGGCTGGGTTCGATGTATTGCTGTGGAACCTGNAAATCAGTGGTT
  TGTTACTGGATCTGCTGACAGAACTATAAAGATCTGGGACTTGGCTAGTGGCANATTAAN
  ACTGTCATTGACTGGGCATATTAGTACTGTGCGGNGCGTGATAGTAAGCACAAGGAGCCC
  ATATCTGTTCTCTTGTGGNAGAGAACAACAAGTGAATGCTGGGATCTCGAATACATAGGT
  ATACGCATTTCATGGCATTNAGTGCAGTGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002669 unedited
  GCGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTAGGGGACAGGGGACAGTTTA
  TTGTAAAATATGAAGCAGCAATGATTGAAGCAAGTGAATTTCTCCTTTATATTCCCAGCC
  AGAGCACAAAATGACTGGATATCCTCATGAACGCCAAGCTTTTTTTTTTTTAATTAAAAA
  GAAAAAAAAAGAGAGAGAAAAAATTGCACATTCATTAAAATCTCTTTCTCTTGATAATTT
  CTGGTTTCCAGCTGACTGGATGAGTTTCTTCTGTGGCTGTGACATACTCTCTGCATACTT
  TAATGGTTTTATCAGCTAGAGCTGTTAACAATGGACTTTGAGACTGATCAAAAGCACAAG
  CAAACATTCCTGATTCACTGACAAAAGACCCAGGTTGCACAATTGCGTGAACTCTCTGAA
  AATTGCACCCAGATCCTCACTGTCCAAACATGCATGGTGCCATTGCCAGGTGCAGGAACA
  AGCACCGCATGATAGGCGACCCGGGACTGTGCTAAAGACACCCTTACCCCCCGCAAGATT
  CTGAGTCGACCTCGCCTATCGCCAAAATCCCTGATCCCAGTTCTCTGCCCACCCCCCCAC
  CCGCCCCCATGTACACCCGCCTACTCACCCCCCCCCCCCCTCTTTCCAACACTGCTATCT
  CCCTCCTCCCCCGCCTGCACAACGCCCACACCCATCTACAACTTCCGCCGACCTCCCGCT
  TACTGCCTCCCCCCCCCCATTCCTCTCACCCCCCCCTACCCTACCTCCCTATAGTGCCCC
  CCCACCCCTATCTCCCCCCACTCACCTCCCCTGTACTACTCCTTTCGCCTCACCCAACTC
  CCCCCCCCCCCACCTCCTCCGCCCCCCCCCCCCCCTATCCTTTACATACCTCATACCTCA
  TCCCTCCCTACCGACCCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002669
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002669.1, NP_002660.1
• RefSeq Size: 1548 bp
• RefSeq ORF: 1545 bp
• Locus ID: 5356
• Cytogenetics: 4q31.3
• Domains: WD40
• Protein Families: Transcription Factors
• Protein Pathways: Spliceosome
• Gene Summary: 'This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.