CDC7 (NM_003503) Human Tagged ORF Clone

CAT#: RC210742

CDC7 (Myc-DDK-tagged)-Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427428) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225970 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC7
• Synonyms: CDC7L1; HsCDC7; Hsk1; huCDC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGG
  CTGAAGGCTCTTTAAAAAAAAACGAGCAGAATTTTAAACTTGCAGGTGTTAAAAAAGATATTGAGAAGCT
  TTATGAAGCTGTACCACAGCTTAGTAATGTGTTTAAGATTGAGGACAAAATTGGAGAAGGCACTTTCAGC
  TCTGTTTATTTGGCCACAGCACAGTTACAAGTAGGACCTGAAGAGAAAATTGCTCTAAAACACTTGATTC
  CAACAAGTCATCCTATAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGATAATGT
  CATGGGAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTATTGCTATGCCATATCTGGAGCATGAG
  TCGTTTTTGGACATTCTGAATTCTCTTTCCTTTCAAGAAGTACGGGAATATATGCTTAATCTGTTCAAAG
  CTTTGAAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTAAGCCCAGCAATTTTTTATATAATAG
  GCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTTTGGCCCAAGGAACCCATGATACGAAAATAGAGCTT
  CTTAAATTTGTCCAGTCTGAAGCTCAGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCACAGGAA
  ACAAGATTCCACTGAGTGGCCCAGTACCTAAGGAGCTGGATCAGCAGTCCACCACAAAAGCTTCTGTTAA
  AAGACCCTACACAAATGCACAAATTCAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAGGCCTT
  TCTGTCCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTTCACATGAGAGCCCTG
  CAGTGAAACTCATGAAGCAGTCAAAGACTGTGGATGTACTGTCTAGAAAGTTAGCAACAAAAAAGAAGGC
  TATTTCTACAAAAGTTATGAATAGTGCTGTGATGAGGAAAACTGCCAGTTCTTGCCCAGCTAGCCTGACC
  TGTGACTGCTATGCAACAGATAAAGTTTGTAGTATTTGCCTTTCAAGGCGTCAGCAGGTTGCCCCTAGGG
  CAGGTACACCAGGATTCAGAGCACCAGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACAT
  GTGGTCTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTATAAAGCAAGTGATGAT
  TTAACTGCTTTGGCCCAAATTATGACAATTAGGGGATCCAGAGAAACTATCCAAGCTGCTAAAACTTTTG
  GGAAATCAATATTATGTAGCAAAGAAGTTCCAGCACAAGACTTGAGAAAACTCTGTGAGAGACTCAGGGG
  TATGGATTCTAGCACTCCCAAGTTAACAAGTGATATACAAGGGCATGCTTCTCATCAACCAGCTATTTCA
  GAGAAGACTGACCATAAAGCTTCTTGCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTA
  AAAAGGGGGATAGTAATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTTAGAAGGCTGGAATGA
  GGTACCTGATGAAGCTTATGACCTGCTTGATAAACTTCTAGATCTAAATCCAGCTTCAAGAATAACAGCA
  GAAGAAGCTTTGTTGCATCCATTTTTTAAAGATATGAGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKIEDKIGEGTFS
  SVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHE
  SFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIEL
  LKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGL
  SVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLT
  CDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDD
  LTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAIS
  EKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITA
  EEALLHPFFKDMSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003503
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003503.2, NM_003503.3, NP_003494.1
• RefSeq Size: 3222 bp
• RefSeq ORF: 1725 bp
• Locus ID: 8317
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• MW: 63.9 kDa
• Gene Summary: This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]