CDC7 (NM_003503) Human Untagged Clone

CAT#: SC323385

CDC7 (untagged)-Kinase deficient mutant (K90M) of Human cell division cycle 7 homolog (S. cerevisiae) (CDC7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC7
• Synonyms: CDC7L1; HsCDC7; Hsk1; huCDC7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003503, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGG
  CTGAAGGCTCTTTAAAAAAAAACGAGCAGAATTTTAAACTTGCAGGTGTTAAAAAAGATATTGAGAAGCT
  TTATGAAGCTGTACCACAGCTTAGTAATGTGTTTAAGATTGAGGACAAAATTGGAGAAGGCACTTTCAGC
  TCTGTTTATTTGGCCACAGCACAGTTACAAGTAGGACCTGAAGAGAAAATTGCTCTAAAACACTTGATTC
  CAACAAGTCATCCTATAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGATAATGT
  CATGGGAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTATTGCTATGCCATATCTGGAGCATGAG
  TCGTTTTTGGACATTCTGAATTCTCTTTCCTTTCAAGAAGTACGGGAATATATGCTTAATCTGTTCAAAG
  CTTTGAAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTAAGCCCAGCAATTTTTTATATAATAG
  GCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTTTGGCCCAAGGAACCCATGATACGAAAATAGAGCTT
  CTTAAATTTGTCCAGTCTGAAGCTCAGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCACAGGAA
  ACAAGATTCCACTGAGTGGCCCAGTACCTAAGGAGCTGGATCAGCAGTCCACCACAAAAGCTTCTGTTAA
  AAGACCCTACACAAATGCACAAATTCAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAGGCCTT
  TCTGTCCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTTCACATGAGAGCCCTG
  CAGTGAAACTCATGAAGCAGTCAAAGACTGTGGATGTACTGTCTAGAAAGTTAGCAACAAAAAAGAAGGC
  TATTTCTACAAAAGTTATGAATAGTGCTGTGATGAGGAAAACTGCCAGTTCTTGCCCAGCTAGCCTGACC
  TGTGACTGCTATGCAACAGATAAAGTTTGTAGTATTTGCCTTTCAAGGCGTCAGCAGGTTGCCCCTAGGG
  CAGGTACACCAGGATTCAGAGCACCAGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACAT
  GTGGTCTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTATAAAGCAAGTGATGAT
  TTAACTGCTTTGGCCCAAATTATGACAATTAGGGGATCCAGAGAAACTATCCAAGCTGCTAAAACTTTTG
  GGAAATCAATATTATGTAGCAAAGAAGTTCCAGCACAAGACTTGAGAAAACTCTGTGAGAGACTCAGGGG
  TATGGATTCTAGCACTCCCAAGTTAACAAGTGATATACAAGGGCATGCTTCTCATCAACCAGCTATTTCA
  GAGAAGACTGACCATAAAGCTTCTTGCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTA
  AAAAGGGGGATAGTAATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTTAGAAGGCTGGAATGA
  GGTACCTGATGAAGCTTATGACCTGCTTGATAAACTTCTAGATCTAAATCCAGCTTCAAGAATAACAGCA
  GAAGAAGCTTTGTTGCATCCATTTTTTAAAGATATGAGCTTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003503 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT
  TCGGCACGAGGGGAAGTGTTGCGCAGGCGCATCCGATCGACTCGCTGCTTTGCTCCCCCTGTGGATGTAA
  CCCCTTAGCTGGCATTTTGCATCTCAATTGGCTTGTGATGGAGGCGTCTTTGGGGATTCAGATGGATGAG
  CCAATGGCTTTTTCTCCCCAGCGTGACCGGTTTCAGGGCTGAAGGGCTCTTTAAAAAAAAAACGAGGCAG
  GAGTTTTTAAACTTGGCAGGTGTTAAAAAAGAATATTGAAGAACCTTTTATGAAAGCCGTTCCCACAGGC
  TTAGTAATGGTGGTTAAAAATGGAGGACAAAATTTGAAAAAGGCCCTTCCACCTCTGGTTTATTTGGCCC
  CCCACCCCATTTCCAATTAGGGACCGGAAAAAAAAAATGGGCCCTATTGCCCTGGATCCCCACCAAGTCC
  TCCCTAAAAAAATTTGCACCTGGACCTCCCGGGCCAAACGGGGGGCGGGGGGGGAAAAAAAAGGGCCTGG
  GGGTTAAAAACCGGGTTTTGAAAAAAAAAACCGGGGTGAAAGCGGCAACCCCATTTTGGGACCCAAGTCC
  CTTTTTTGGAATTTTGAAAATTCTTTTCCCTTTCCCAAAATTCGGGGAAAATGCCCCTAATCGTTCTAAA
  CCTTTGAACCGCTTCTCACATGTTTTGGTTGTGTCTCCGGTGTTTTCCCCCCCCATTTTTTTAATAAAAC
  GCCCCTGAAAATATCGCTGATGGGAACTAGTGTTGTGCCCAGAGCCCCACTAATAACAAAAGAACATCTT
  ATTTTTCGCCCTAACTACGCACAAGAAGAATTGTACCCAAAACCCTAAAG
  
  Kinase domain sequence

  >SC323385 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATGMGCAATGGGCGGTAGSGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCAC
  GAGGGGAAGTGTTGCGCAGGCGCATCCGATCGACTCGCTGCTTTGCTCCCCCTGTGGATGTAACCCCTTA
  GCTGGCATTTTGCATCTCAATTGGCTTGTGATGGAGGCGTCTTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_003503
• ORF Size: 1725 bp
• Insert Size: 3100
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003503.2, NP_003494.1
• RefSeq Size: 3178
• RefSeq ORF: 1725
• Locus ID: 8317
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Gene Summary: This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the most commonly occurring transcript. Variants 1, 2, and 3 all encode the same protein.