TRPM5 (NM_014555) Human Tagged ORF Clone

CAT#: RC210840

TRPM5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5)

Product Images

Western blot validation of overexpression lysate (Cat# LY415198) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210840 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM5
• Synonyms: LTRPC5; MTR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210840 representing NM_014555
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGATGTCCAAGGCCCCCGTCCCGGAAGCCCCGGGGATGCTGAAGACCGGCGGGAGCTGGGCTTGC
  ACAGGGGCGAGGTCAACTTTGGAGGGTCTGGGAAGAAGCGAGGCAAGTTTGTACGGGTGCCGAGCGGAGT
  GGCCCCGTCTGTGCTCTTTGACCTGCTGCTTGCTGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC
  CTGGTGGGTGAGGAGCAGCCTTTCGCCATGAAGTCCTGGCTGCGGGATGTGCTGCGCAAGGGGCTGGTGA
  AGGCGGCTCAGAGCACAGGAGCCTGGATCCTGACCAGTGCCCTCCGCGTGGGCCTGGCCAGGCATGTCGG
  GCAGGCCGTGCGCGACCACTCGCTGGCCAGCACGTCCACCAAGGTCCGTGTGGTTGCTGTCGGCATGGCC
  TCGCTGGGCCGCGTCCTGCACCGCCGCATTCTGGAGGAGGCCCAGGAGGATTTTCCTGTCCACTACCCTG
  AGGATGACGGCGGCAGCCAGGGCCCCCTCTGTTCACTGGACAGCAACCTCTCCCACTTCATCCTGGTGGA
  GCCAGGCCCCCCGGGGAAGGGCGATGGGCTGACGGAGCTGCGGCTGAGGCTGGAGAAGCACATCTCGGAG
  CAGAGGGCGGGCTACGGGGGCACTGGCAGCATCGAGATCCCTGTCCTCTGCTTGCTGGTCAATGGTGATC
  CCAACACCTTGGAGAGGATCTCCAGGGCCGTGGAGCAGGCTGCCCCGTGGCTGATCCTGGCAGGCTCGGG
  GGGCATCGCCGATGTGCTTGCTGCCCTAGTGAACCAGCCCCACCTCCTGGTGCCCAAGGTGGCCGAGAAG
  CAGTTTAAGGAGAAGTTCCCCAGCAAGCATTTCTCTTGGGAGGACATCGTGCGCTGGACCAAGCTGCTGC
  AGAACATCACCTCACACCAGCACCTGCTCACCGTGTATGACTTCGAGCAGGAGGGCTCCGAGGAGCTGGA
  CACGGTCATCCTGAAGGCGCTGGTGAAAGCCTGCAAGAGCCACAGCCAGGAGCCTCAGGACTATCTGGAT
  GAGCTCAAGCTGGCCGTGGCCTGGGACCGCGTGGACATCGCCAAGAGTGAGATCTTCAATGGGGACGTGG
  AGTGGAAGTCCTGTGACCTGGAGGAGGTGATGGTGGACGCCCTGGTCAGCAACAAGCCCGAGTTTGTGCG
  CCTCTTTGTGGACAACGGCGCAGACGTGGCCGACTTCCTGACGTATGGGCGGCTGCAGGAGCTCTACCGC
  TCTGTGTCACGCAAGAGCCTGCTCTTCGACCTGCTGCAGCGGAAGCAGGAGGAGGCCCGGCTGACGCTGG
  CCGGCCTGGGCACCCAGCAGGCCCGGGAGCCACCCGCGGGGCCACCGGCCTTCTCCCTGCACGAGGTCTC
  CCGCGTACTCAAGGACTTCCTGCAGGACGCCTGCCGAGGCTTCTACCAGGACGGCCGGCCAGGGGACCGC
  AGGAGGGCGGAGAAGGGCCCGGCCAAGCGGCCCACGGGCCAGAAGTGGCTGCTGGACCTGAACCAGAAGA
  GCGAGAACCCCTGGCGGGACCTGTTCCTGTGGGCCGTGCTGCAGAACCGCCACGAGATGGCCACCTACTT
  CTGGGCCATGGGCCAGGAAGGTGTGGCAGCCGCACTGGCCGCCTGCAAAATCCTCAAAGAGATGTCGCAC
  CTGGAGACGGAGGCCGAGGCGGCCCGAGCCACGCGCGAGGCGAAATACGAGCGGCTGGCCCTTGACCTCT
  TCTCCGAGTGCTACAGCAACAGTGAGGCCCGCGCCTTCGCCCTGCTGGTGCGCCGGAACCGCTGCTGGAG
  CAAGACCACCTGCCTGCACCTGGCCACCGAGGCTGACGCCAAGGCCTTCTTTGCCCACGACGGCGTTCAG
  GCCTTCCTGACCAGGATCTGGTGGGGGGACATGGCCGCAGGCACGCCCATCCTGCGGCTGCTAGGAGCCT
  TCCTCTGCCCCGCCCTCGTCTATACCAACCTCATCACCTTCAGTGAGGAAGCTCCCCTGAGGACAGGCCT
  GGAGGACCTGCAGGACCTGGACAGCCTGGACACGGAGAAGAGCCCGCTGTATGGCCTGCAGAGCCGGGTG
  GAGGAGCTGGTGGAGGCGCCGAGGGCTCAGGGTGACCGAGGCCCACGTGCTGTCTTCCTGCTCACACGCT
  GGCGGAAATTCTGGGGCGCTCCCGTGACTGTGTTCCTGGGGAACGTGGTCATGTACTTCGCCTTCCTCTT
  CCTGTTCACCTACGTCCTGCTGGTGGACTTCAGGCCGCCCCCCCAGGGCCCCTCAGGGCCCGAGGTCACC
  CTCTACTTCTGGGTCTTTACGCTGGTGCTGGAGGAAATCCGGCAGGGCTTCTTCACAGACGAGGACACAC
  ACCTGGTGAAGAAGTTCACACTGTATGTGGGGGACAACTGGAACAAGTGTGACATGGTGGCCATCTTCCT
  GTTCATCGTGGGTGTCACCTGCAGGATGCTGCCGTCGGCGTTTGAGGCTGGCCGCACAGTCCTCGCCATG
  GACTTCATGGTGTTCACGCTGCGGCTGATCCATATCTTTGCCATACACAAGCAGCTGGGCCCCAAGATCA
  TCGTGGTAGAGCGCATGATGAAGGACGTCTTCTTCTTCCTCTTCTTTCTGAGCGTGTGGCTCGTGGCCTA
  CGGTGTCACCACCCAGGCGCTGCTGCACCCCCATGACGGCCGCCTGGAGTGGATCTTCCGCCGGGTGCTC
  TACCGGCCCTACCTGCAGATCTTCGGCCAGATCCCACTGGACGAGATTGATGAAGCCCGTGTGAACTGCT
  CCACCCACCCACTGCTGCTGGAGGACTCACCATCCTGCCCCAGCCTCTATGCCAACTGGCTGGTCATCCT
  CCTGCTGGTCACCTTCCTGTTGGTCACCAATGTGCTGCTCATGAACCTGCTCATCGCCATGTTCAGCTAC
  ACGTTCCAGGTGGTGCAGGGCAACGCAGACATGTTCTGGAAGTTCCAGCGCTACAACCTGATTGTGGAGT
  ACCACGAGCGCCCCGCCCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGACGCTCCGCCGGGT
  CTTCAAGAAGGAGGCTGAGCACAAGCGGGAGCACCTGGAGAGAGACCTGCCAGACCCCCTGGACCAGAAG
  GTCGTCACCTGGGAGACAGTCCAGAAGGAGAACTTCCTGAGCAAGATGGAGAAGCGGAGGAGGGACAGCG
  AGGGGGAGGTGCTGCGGAAAACCGCCCACAGAGTGGACTTCATTGCCAAGTACCTCGGGGGTCTGAGAGA
  GCAAGAAAAGCGCATCAAGTGTCTGGAGTCACAGATCAACTACTGCTCGGTGCTCGTGTCCTCCGTGGCT
  GACGTGCTGGCCCAGGGTGGCGGCCCCCGGAGCTCTCAGCACTGTGGCGAGGGAAGCCAGCTGGTGGCTG
  CTGACCACAGAGGTGGTTTAGATGGCTGGGAACAACCCGGGGCTGGCCAGCCTCCCTCGGACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210840 representing NM_014555
  Red=Cloning site Green=Tags(s)
  
  MQDVQGPRPGSPGDAEDRRELGLHRGEVNFGGSGKKRGKFVRVPSGVAPSVLFDLLLAEWHLPAPNLVVS
  LVGEEQPFAMKSWLRDVLRKGLVKAAQSTGAWILTSALRVGLARHVGQAVRDHSLASTSTKVRVVAVGMA
  SLGRVLHRRILEEAQEDFPVHYPEDDGGSQGPLCSLDSNLSHFILVEPGPPGKGDGLTELRLRLEKHISE
  QRAGYGGTGSIEIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVNQPHLLVPKVAEK
  QFKEKFPSKHFSWEDIVRWTKLLQNITSHQHLLTVYDFEQEGSEELDTVILKALVKACKSHSQEPQDYLD
  ELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMVDALVSNKPEFVRLFVDNGADVADFLTYGRLQELYR
  SVSRKSLLFDLLQRKQEEARLTLAGLGTQQAREPPAGPPAFSLHEVSRVLKDFLQDACRGFYQDGRPGDR
  RRAEKGPAKRPTGQKWLLDLNQKSENPWRDLFLWAVLQNRHEMATYFWAMGQEGVAAALAACKILKEMSH
  LETEAEAARATREAKYERLALDLFSECYSNSEARAFALLVRRNRCWSKTTCLHLATEADAKAFFAHDGVQ
  AFLTRIWWGDMAAGTPILRLLGAFLCPALVYTNLITFSEEAPLRTGLEDLQDLDSLDTEKSPLYGLQSRV
  EELVEAPRAQGDRGPRAVFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGPEVT
  LYFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVGDNWNKCDMVAIFLFIVGVTCRMLPSAFEAGRTVLAM
  DFMVFTLRLIHIFAIHKQLGPKIIVVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVL
  YRPYLQIFGQIPLDEIDEARVNCSTHPLLLEDSPSCPSLYANWLVILLLVTFLLVTNVLLMNLLIAMFSY
  TFQVVQGNADMFWKFQRYNLIVEYHERPALAPPFILLSHLSLTLRRVFKKEAEHKREHLERDLPDPLDQK
  VVTWETVQKENFLSKMEKRRRDSEGEVLRKTAHRVDFIAKYLGGLREQEKRIKCLESQINYCSVLVSSVA
  DVLAQGGGPRSSQHCGEGSQLVAADHRGGLDGWEQPGAGQPPSDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014555
• ORF Size: 3495 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014555.1, NM_014555.2, NM_014555.3, NP_055370.1
• RefSeq Size: 3913
• RefSeq ORF: 3498
• Locus ID: 29850
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Protein Pathways: Taste transduction
• MW: 131.3 kDa
• Gene Summary: This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. [provided by RefSeq, Oct 2010]