POU2F3 (NM_014352) Human Tagged ORF Clone

CAT#: RC210969

POU2F3 (Myc-DDK-tagged)-Human POU class 2 homeobox 3 (POU2F3)

Product Images

Western blot validation of overexpression lysate (Cat# LY415336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210969 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified POU2F3 protein (Cat# TP310969). The protein was produced from HEK293T cells transfected with POU2F3 cDNA clone (Cat# RC210969) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POU2F3
• Synonyms: Epoc-1; OCT-11; OCT11; OTF-11; PLA-1; PLA1; Skn-1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210969 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAATCTGGAGTCCATGCACACAGATATCAAGATGAGTGGGGATGTAGCCGATTCCACGGATGCTC
  GCAGCACTCTCAGCCAGGTGGAGCCAGGAAATGATCGAAATGGCCTAGATTTCAACAGGCAGATTAAAAC
  CGAAGATCTCAGTGACTCCCTGCAGCAGACCCTCTCCCATCGGCCATGCCACCTGAGTCAAGGACCTGCC
  ATGATGTCCGGAAACCAAATGTCTGGGCTAAATGCCAGCCCATGTCAGGACATGGCTTCCCTCCATCCGC
  TCCAGCAGCTTGTGCTGGTTCCCGGCCACTTACAGTCTGTATCCCAGTTCCTGCTATCTCAGACCCAGCC
  TGGGCAGCAAGGTCTGCAGCCAAATCTCCTCCCCTTTCCACAGCAACAAAGCGGTCTCCTCCTCCCACAG
  ACTGGGCCGGGACTGGCATCCCAGGCATTTGGGCACCCTGGGCTGCCAGGATCCTCTTTAGAACCCCACC
  TGGAAGCATCCCAGCATCTCCCAGTGCCCAAGCATCTACCCAGCTCTGGAGGGGCCGATGAGCCCAGTGA
  CCTCGAGGAGCTGGAGAAGTTTGCCAAGACCTTCAAGCAGAGGCGCATTAAGCTGGGCTTCACACAGGGA
  GATGTGGGGCTGGCGATGGGAAAGCTGTATGGCAACGACTTCAGCCAGACCACCATCTCACGATTTGAGG
  CCCTCAACCTGAGCTTCAAGAACATGTGCAAGCTCAAGCCCCTGCTGGAGAAGTGGCTGAATGATGCAGA
  GTCCTCTCCGTCAGACCCCTCAGTGAGCACGCCCAGCTCCTACCCCAGCCTCAGTGAAGTATTTGGTAGG
  AAGAGAAAGAAACGGACCAGCATCGAGACCAACATCCGCCTGACTCTGGAGAAGAGGTTTCAAGATAACC
  CAAAACCCAGCTCGGAGGAGATCTCCATGATTGCAGAGCAGTTGTCCATGGAGAAGGAGGTGGTGAGGGT
  CTGGTTCTGCAACCGACGCCAAAAGGAGAAGCGAATCAACTGCCCTGTGGCCACACCCATCAAACCACCT
  GTCTACAACTCCCGGCTGGTATCTCCCTCAGGGTCTCTGGGCCCCCTCTCTGTCCCTCCTGTCCACAGTA
  CCATGCCTGGAACAGTAACGTCATCCTGTTCCCCTGGGAACAACAGCAGGCCTTCATCTCCTGGCTCAGG
  ACTCCACGCCAGCAGCCCCACTGCATCTCAAAATAACTCCAAAGCAGCAGTGAACTCCGCCTCCAGTTTT
  AACTCTTCAGGATCTTGGTACCGATGGAATCATTCCACCTACCTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210969 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVNLESMHTDIKMSGDVADSTDARSTLSQVEPGNDRNGLDFNRQIKTEDLSDSLQQTLSHRPCHLSQGPA
  MMSGNQMSGLNASPCQDMASLHPLQQLVLVPGHLQSVSQFLLSQTQPGQQGLQPNLLPFPQQQSGLLLPQ
  TGPGLASQAFGHPGLPGSSLEPHLEASQHLPVPKHLPSSGGADEPSDLEELEKFAKTFKQRRIKLGFTQG
  DVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAESSPSDPSVSTPSSYPSLSEVFGR
  KRKKRTSIETNIRLTLEKRFQDNPKPSSEEISMIAEQLSMEKEVVRVWFCNRRQKEKRINCPVATPIKPP
  VYNSRLVSPSGSLGPLSVPPVHSTMPGTVTSSCSPGNNSRPSSPGSGLHASSPTASQNNSKAAVNSASSF
  NSSGSWYRWNHSTYLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014352
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014352.1, NM_014352.2, NM_014352.3, NP_055167.1, NP_055167.2
• RefSeq Size: 3013 bp
• RefSeq ORF: 1311 bp
• Locus ID: 25833
• MW: 47.4 kDa
• Gene Summary: This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]