Pur B (PURB) (NM_033224) Human Tagged ORF Clone

CAT#: RC211001

PURB (Myc-DDK-tagged)-Human purine-rich element binding protein B (PURB)

Product Images

Western blot validation of overexpression lysate (Cat# LY409642) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211001 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PURB
• Synonyms: PURBETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211001 representing NM_033224
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGCGACAGCGGCAGCGAGCGCGGCGGCGGCGGTGGGCCGTGCGGGTTCCAGCCCGCGTCCC
  GCGGCGGCGGCGAGCAAGAGACGCAGGAGCTGGCCTCGAAGCGGCTGGACATCCAGAACAAGCGCTTCTA
  CTTAGATGTGAAGCAGAACGCCAAGGGCCGCTTCCTCAAGATCGCCGAGGTGGGCGCGGGCGGTTCCAAG
  AGCCGCCTCACGCTGTCCATGGCGGTGGCCGCCGAGTTCCGCGACTCGCTGGGCGACTTCATAGAACACT
  ACGCGCAGCTGGGCCCTAGCAGCCCCGAGCAGCTGGCGGCTGGCGCCGAGGAGGGCGGCGGGCCGCGGCG
  CGCGCTCAAGAGCGAATTCTTGGTGCGTGAGAACCGCAAGTACTACCTGGACCTCAAGGAGAACCAGCGC
  GGCCGCTTCCTGCGCATCCGCCAAACGGTCAACCGCGGCGGTGGCGGCTTCGGCGCGGGCCCCGGGCCGG
  GCGGCTTGCAGAGCGGCCAGACCATCGCGCTGCCTGCGCAGGGCCTCATCGAGTTCCGCGACGCGCTGGC
  GAAGCTCATAGACGACTACGGAGGCGAGGACGACGAGCTGGCAGGCGGCCCGGGAGGCGGCGCCGGGGGC
  CCAGGGGGCGGCCTGTATGGAGAGCTCCCGGAGGGCACCTCCATCACCGTGGACTCCAAGCGCTTCTTCT
  TCGATGTGGGCTGCAACAAATACGGGGTGTTTCTGCGAGTGAGCGAGGTGAAGCCGTCCTACCGCAATGC
  CATCACCGTACCCTTCAAAGCCTGGGGCAAGTTCGGAGGCGCCTTTTGCCGGTATGCGGATGAGATGAAA
  GAAATCCAGGAACGACAGAGGGATAAGCTTTATGAGCGACGTGGTGGGGGCAGCGGCGGCGGCGAAGAGT
  CAGAGGGTGAGGAGGTGGATGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211001 representing NM_033224
  Red=Cloning site Green=Tags(s)
  
  MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSK
  SRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQR
  GRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGG
  PGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMK
  EIQERQRDKLYERRGGGSGGGEESEGEEVDED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033224
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033224.1, NM_033224.2, NM_033224.3, NM_033224.4, NP_150093.1
• RefSeq Size: 9074 bp
• RefSeq ORF: 939 bp
• Locus ID: 5814
• Cytogenetics: 7p13
• Domains: PUR
• MW: 33.1 kDa
• Gene Summary: 'This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]'