Pur B (PURB) (NM_033224) Human Untagged Clone

CAT#: SC101337

PURB (untagged)-Human purine-rich element binding protein B (PURB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PURB
• Synonyms: PURBETA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033224, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACGGCGACAGCGGCAGCGAGCGCGGCGGCGGCGGTGGGCCGTGCGGGTTCCAGCCCGCGTCCC
  GCGGCGGCGGCGAGCAAGAGACGCAGGAGCTGGCCTCGAAGCGGCTGGACATCCAGAACAAGCGCTTCTA
  CTTAGATGTGAAGCAGAACGCCAAGGGCCGCTTCCTCAAGATCGCCGAGGTGGGCGCGGGCGGTTCCAAG
  AGCCGCCTCACGCTGTCCATGGCGGTGGCCGCCGAGTTCCGCGACTCGCTGGGCGACTTCATAGAACACT
  ACGCGCAGCTGGGCCCTAGCAGCCCCGAGCAGCTGGCGGCTGGCGCCGAGGAGGGCGGCGGGCCGCGGCG
  CGCGCTCAAGAGCGAATTCTTGGTGCGTGAGAACCGCAAGTACTACCTGGACCTCAAGGAGAACCAGCGC
  GGCCGCTTCCTGCGCATCCGCCAAACGGTCAACCGCGGCGGTGGCGGCTTCGGCGCGGGCCCCGGGCCGG
  GCGGCTTGCAGAGCGGCCAGACCATCGCGCTGCCTGCGCAGGGCCTCATCGAGTTCCGCGACGCGCTGGC
  GAAGCTCATAGACGACTACGGAGGCGAGGACGACGAGCTGGCAGGCGGCCCGGGAGGCGGCGCCGGGGGC
  CCAGGGGGCGGCCTGTATGGAGAGCTCCCGGAGGGCACCTCCATCACCGTGGACTCCAAGCGCTTCTTCT
  TCGATGTGGGCTGCAACAAATACGGGGTGTTTCTGCGAGTGAGCGAGGTGAAGCCGTCCTACCGCAATGC
  CATCACCGTACCCTTCAAAGCCTGGGGCAAGTTCGGAGGCGCCTTTTGCCGGTATGCGGATGAGATGAAA
  GAAATCCAGGAACGACAGAGGGATAAGCTTTATGAGCGACGTGGTGGGGGCAGCGGCGGCGGCGAAGAGT
  CAGAGGGTGAGGAGGTGGATGAGGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033224 unedited
  GCCGCGAATTCGGCACGAGGCGCGGCGGGCGGTGGCGGCGAGGCGGCGGCCTAGAAGATG
  GCGGACGGCGACAGCGGCAGCGAGCGCGGCGGCGGCGGTGGGCCGTGCGGGTTCCAGCCC
  GCGTCCCGCGGCGGCGGCGAGCAAGAGACGCAGGAGCTGGCCTCGAAGCGGCTGGACATC
  CAGAACAAGCGCTTCTACTTAGATGTGAAGCAGAACGCCAAGGGCCGCTTCCTCAAGATC
  GCCGAGGTGGGCGCGGGCGGTTCCAAGAGCCGCCTCACGCTGTCCATGGCGGTGGCCGCC
  GAGTTCCGCGACTCGCTGGGCGACTTCATAGAACACTACGCGCAGCTGGGCCCTAGCAGC
  CCCGAGCAGCTGGCGGCTGGCGCCGAGGAGGGCGGCGGGCCGCGGCGCGCGCTCAAGAGC
  GAATTCTTGGTGCGTGAGAACCGCAAGTACTACCTGGACCTCAAGGAGAACCAGCGCGGC
  CGCTTCCTGCGCATCCGCCAAACGGTCAACCGCGGCGGTGGCGGCTTCGGCGCGGGCCCC
  GGGCCGGGCGGCTTGCAGAGCGGCCAGACCATCGCGCTGCCTGCGCAGGGCCTCATCGAG
  TTCCGCGACGCGCTGGCGAAGCTCATAGACGACTACGGAGGCGAGGACGACGAGCTGGCA
  GGCGGCCCGGGAGGCGGCGCCGGGGGCCCAGGGGGCGGCCTGTATGGAGAGCTCCCGGAG
  GGGCACCTCATCACCGTGGGACTCCAGCGCTTCTTCTTCGATGTGGGCTGCACAAATACN
  GGGGTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033224 unedited
  CCCCCTACCTTGGACCCGGCCGCAACCTACGAGCGAGTTTTTTTTTTTTTTTTTTTTTCT
  TTTCTACAAACTATCTCCCGCCCTCCCCAGCCCCACCCACACACCCATTATCCAGTATAC
  TGAATGGGAGTTGTAACTCTTTAGGAAGACAGCTAATTGATTTACAGTACTCTAAAACCA
  ACCCTGAAAACTGATAATCTCATTCACTTACTATAACTTCAGTGCTGTAGTTGACAGGAA
  AGAAAAAGTTACCTTAAGGCTCAGGGGAAAGTAACTTATTAAATTGTGAATGCAGCATTA
  TTTACTCTCCCATCCATTGGAAACTAGCAAAATGCATTCTGGATCAATAATACAAACCTT
  CTAACATTAGCAAAGATTTCTACTCTTCTTACACAGAGTTAGAACAATATTCATAAGCAA
  TTATCTAAGGGAGCCTGAGAAACTATGATCCACATCACACAAAGATTTCATGTATTTCAT
  GATCAAAAAGTGCTTTATCTTTGAGAAATGTCAGGGTTACAATGGAAATATACAATGTAA
  AGTCACTTGATATTTTTTCAAGTGAAGTACAAAGTAGAAAAGGAGCAAAAAGATGGAGGC
  TCTGCTCTCTGAACAGGCCTTATGCATGTGACTTTTAGGGTGTACGAAATTAAAGCTGTT
  TTACGAATTAAACATAGTGGGACCTTTCCCCACTATATTCTAAAACCTGACATTGGCCAT
  CAAACAGTCCACTTTGTTATTATTAAACCCATCTGACCCTTCTAACACATACCACACTCC
  ACTGATTCACTCCAGGGTCAAAAACATTACTTTTCCCCACCCACAAGAACACCGACCCTC
  CCATTAACTCCAGACTGTGCCACACCAAAACTCCCCTCCACGACCCTCCCTCCTCTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033224
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033224.3, NP_150093.1
• RefSeq Size: 9074 bp
• RefSeq ORF: 939 bp
• Locus ID: 5814
• Cytogenetics: 7p13
• Domains: PUR
• Gene Summary: 'This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]'