COMP (NM_000095) Human Tagged ORF Clone

CAT#: RC211080L4

Lenti ORF clone of Human cartilage oligomeric matrix protein (COMP), mGFP tagged

Product Images

Double digestion of RC211080L4 using SgfI and RsrII

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: COMP
• Synonyms: EDM1; EPD1; MED; PSACH; THBS5; TSP5
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC211080).
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene
• ACCN: NM_000095
• ORF Size: 2271 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000095.2, NP_000086.2
• RefSeq Size: 2471 bp
• RefSeq ORF: 2274 bp
• Locus ID: 1311
• UniProt ID: P49747
• Cytogenetics: 19p13.11
• Domains: EGF_CA, tsp_3, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
• MW: 82.9 kDa
• Gene Summary: 'The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]'