COMP (NM_000095) Human Untagged Clone

CAT#: SC116330

COMP (untagged)-Human cartilage oligomeric matrix protein (COMP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COMP
• Synonyms: EDM1; EPD1; MED; PSACH; THBS5; TSP5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116330 sequence for NM_000095 edited (data generated by NextGen Sequencing)
  ATGGTCCCCGACACCGCCTGCGTTCTTCTGCTCACCCTGGCTGCCCTCGGCGCGTCCGGA
  CAGGGCCAGAGCCCGTTGGGCTCAGACCTGGGCCCGCAGATGCTTCGGGAACTGCAGGAA
  ACCAACGCGGCGCTGCAGGACGTGCGGGAGCTGCTGCGGCAGCAGGTCAGGGAGATCACG
  TTCCTGAAAAACACGGTGATGGAGTGTGACGCGTGCGGGATGCAGCAGTCAGTACGCACC
  GGCCTACCCAGCGTGCGGCCCCTGCTCCACTGCGCGCCCGGCTTCTGCTTCCCCGGCGTG
  GCCTGCATCCAGACGGAGAGCGGCGCGCGCTGCGGCCCCTGCCCCGCGGGCTTCACGGGC
  AACGGCTCGCACTGCACCGACGTCAACGAGTGCAACGCCCACCCCTGCTTCCCCCGAGTC
  CGCTGTATCAACACCAGCCCGGGGTTCCGCTGCGAGGCTTGCCCGCCGGGGTACAGCGGC
  CCCACCCACCAGGGCGTGGGGCTGGCTTTCGCCAAGGCCAACAAGCAGGTTTGCACGGAC
  ATCAACGAGTGTGAGACCGGGCAACATAACTGCGTCCCCAACTCCGTGTGCATCAACACC
  CGGGGCTCCTTCCAGTGCGGCCCGTGCCAGCCCGGCTTCGTGGGCGACCAGGCGTCCGGC
  TGCCAGCGGCGCGCACAGCGCTTCTGCCCCGACGGCTCGCCCAGCGAGTGCCACGAGCAT
  GCAGACTGCGTCCTAGAGCGCGATGGCTCGCGGTCGTGCGTGTGTGCCGTTGGCTGGGCC
  GGCAACGGGATCCTCTGTGGTCGCGACACTGACCTAGACGGCTTCCCGGACGAGAAGCTG
  CGCTGCCCGGAGCGCCAGTGCCGTAAGGACAACTGCGTGACTGTGCCCAACTCAGGGCAG
  GAGGATGTGGACCGCGATGGCATCGGAGACGCCTGCGATCCGGATGCCGACGGGGACGGG
  GTCCCCAATGAAAAGGACAACTGCCCGCTGGTGCGGAACCCAGACCAGCGCAACACGGAC
  GAGGACAAGTGGGGCGATGCGTGCGACAACTGCCGGTCCCAGAAGAACGACGACCAAAAG
  GACACAGACCAGGACGGCCGGGGCGATGCGTGCGACGACGACATCGACGGCGACCGGATC
  CGCAACCAGGCCGACAACTGCCCTAGGGTACCCAACTCAGACCAGAAGGACAGTGATGGC
  GATGGTATAGGGGATGCCTGTGACAACTGTCCCCAGAAGAGCAACCCGGATCAGGCGGAT
  GTGGACCACGACTTTGTGGGAGATGCTTGTGACAGCGATCAAGACCAGGATGGAGACGGA
  CATCAGGACTCTCGGGACAACTGTCCCACGGTGCCTAACAGTGCCCAGGAGGACTCAGAC
  CACGATGGCCAGGGTGATGCCTGCGACGACGACGACGACAATGACGGAGTCCCTGACAGT
  CGGGACAACTGCCGCCTGGTGCCTAACCCCGGCCAGGAGGACGCGGACAGGGACGGCGTG
  GGCGACGTGTGCCAGGACGACTTTGATGCAGACAAGGTGGTAGACAAGATCGACGTGTGT
  CCGGAGAACGCTGAAGTCACGCTCACCGACTTCAGGGCCTTCCAGACAGTCGTGCTGGAC
  CCGGAGGGTGACGCGCAGATTGACCCCAACTGGGTGGTGCTCAACCAGGGAAGGGAGATC
  GTGCAGACAATGAACAGCGACCCAGGCCTGGCTGTGGGTTACACTGCCTTCAATGGCGTG
  GACTTCGAGGGCACGTTCCATGTGAACACGGTCACGGATGACGACTATGCGGGCTTCATC
  TTTGGCTACCAGGACAGCTCCAGCTTCTACGTGGTCATGTGGAAGCAGATGGAGCAAACG
  TATTGGCAGGCGAACCCCTTCCGTGCTGTGGCCGAGCCTGGCATCCAACTCAAGGCTGTG
  AAGTCTTCCACAGGCCCCGGGGAACAGCTGCGGAACGCTCTGTGGCATACAGGAGACACA
  GAGTCCCAGGTGCGGCTGCTGTGGAAGGACCCGCGAAACGTGGGTTGGAAGGACAAGAAG
  TCCTATCGTTGGTTCCTGCAGCACCGGCCCCAAGTGGGCTACATCAGGGTGCGATTCTAT
  GAGGGCCCTGAGCTGGTGGCCGACAGCAACGTGGTCTTGGACACAACCATGCGGGGTGGC
  CGCCTGGGGGTCTTCTGCTTCTCCCAGGAGAACATCATCTGGGCCAACCTGCGTTACCGC
  TGCAATGACACCATCCCAGAGGACTATGAGACCCATCAGCTGCGGCAAGCCTAG
  
  Clone variation with respect to NM_000095.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000095 unedited
  NGGGTTCAAATTAGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCAGCT
  CCCCGCCACCGCCATGGTCCCCGACACCGCCTGCGTTCTTCTGCTCACCCTGGCTGCCCT
  CGGCGCGTCCGGACAGGGCCAGAGCCCGTTGGGCTCAGACCTGGGCCCGCAGATGCTTCG
  GGAACTGCAGGAAACCAACGCGGCGCTGCAGGACGTGCGGGAGCTGCTGCGGCAGCAGGT
  CAGGGAGATCACGTTCCTGAAAAACACGGTGATGGAGTGTGACGCGTGCGGGATGCAGCA
  GTCAGTACGCACCGGCCTACCCAGCGTGCGGCCCCTGCTCCACTGCGCGCCCGGCTTCTG
  CTTCCCCGGCGTGGCCTGCATCCAGACGGAGAGCGGCGCGCGCTGCGGCCCCTGCCCCGC
  GGGCTTCACGGGCAACGGCTCGCACTGCACCGACGTCAACGAGTGCAACGCCCACCCCTG
  CTTCCCCCGAGTCCGCTGTATCAACACCAGCCCGGGGTTCCGCTGCGAGGCTTGCCCGCC
  GGGGTACAGCGGCCCCACCCACCAGGGCGTGGGGCTGGCTTTCGCCAAGGCCAACAAGCA
  GGTTTGCACGGACATCAACGAGTGTGAGACCGGGCAACATAACTGCGTCCCCAACTCCGT
  GTGCATCAACACCCGGNGCTCCTTCCAGTGCGGCCCGTGCCAGCCCGGCTTCGTGGGCGA
  CCAGGCGTCCGGCTGCCAGCGGCGCGCACAGCGCTTCTGCCCCGACGGCTCGCCCAGCGA
  GTGCCACGAGCATGCAGACTGCGTCCTANAGCGCGATGGCTCGCGGTCGTGCGTGTGTGC
  CGTTGGGCTGGCCGCAACGGGATCCTCTGTGGTCGCGACACTGACCTANACGGCTTCCCG
  GACGAAAGCTGCGCTGCCCGGAGCGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000095 unedited
  GGAACTTCCGCACTTTNATTCTGTCCTCTCTGGACCCTTCTCACTTCCCCCTCAGGCGGC
  CACCCCTTGGGGCTGGGTGCAGAGCCCCCATCCAGCCGCGGTGAGGGTGGCTGTCATCCG
  GCGGGTCCTCACCCTGGTCCCTAGGCTTGCCGCAGCTGATGGGTCTCATAGTCCTCTGGG
  ATGGTGTCATTGCAGCGGTAACGCAGGTTGGCCCAGATGATGTTCTCCTGGGAGAAGCAG
  AAGACCCCCAGGCGGCCACCCCGCATGGTTGTGTCCAAGACCACGTTGCTGTCGGCCACC
  AGCTCAGGGCCCTCATAGAATCGCACCCTGATGTAGCCCACTTGGGGCCGGTGCTGCAGG
  AACCAACGATAGGACTTCTTGTCCTTCCAACCCACGTTTCGCGGGTCCTTCCACAGCAGC
  CGCACCTGGGACTCTGTGTCTCCTGTATGCCACAGAGCGTTCCGCAGCTGTTCCCCGGGG
  CCTGTGGAAGACTTCACAGCCTTGAGTTGGATGCCAGGCTCGGCCACAGCACGGAAGGGG
  TTCGCCTGCCAATACGTTTGCTCCATCTGCTTCCACATGACCACGTAGAAGCTGGAGCTG
  TCCTGGTAGCCAAAGATGAAGCCCGCATAGTCGTCATCCGTGACCGTGTTCACATGGAAC
  GTGCCCTCGAAGTCCACGCCATTGAAGGCAGTGTAACCCACAGCCAGCCCTGGGTCGCTG
  TTCATTGTCTGCACGATCTCCCTTCCCTGGTTGAACACCACCCATTGGGGTCAATCTGCG
  CGTTACCCTCCGGGTCCAACACCACTGTTTGGGAAGCCCCTGAAGTCGGTGAGCGTGACT
  TCAGCGTTTCTCCGACACACGTGATCTTGTGTACCACCTTNTCTGCATAAAATCGGCCCG
  GGACCCGTTGCCCAGCCCGTCCCGGACCGTACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000095
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000095.2, NP_000086.2
• RefSeq Size: 2471 bp
• RefSeq ORF: 2274 bp
• Locus ID: 1311
• Cytogenetics: 19p13.11
• Domains: EGF_CA, tsp_3, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]'