EIF3B (NM_003751) Human Tagged ORF Clone

CAT#: RC211093

EIF3B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit B (EIF3B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418454) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211093 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EIF3B protein (Cat# TP311093). The protein was produced from HEK293T cells transfected with EIF3B cDNA clone (Cat# RC211093) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF3B
• Synonyms: EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211093 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAGGAGCGCGCCGAGCCCGGCCAGCAGCAGC
  CGGCCGCCGAGCCGCCGCCAGCCGAGGGGCTGCTGCGGCCCGCGGGGCCCGGCGCTCCGGAGGCCGCGGG
  GACCGAGGCCTCCAGTGAGGAGGTGGGGATCGCGGAGGCCGGGCCGGAGCCCGAGGTGAGGACCGAGCCG
  GCGGCCGAGGCAGAGGCGGCCTCCGGCCCGTCCGAGTCGCCCTCGCCGCCGGCCGCCGAGGAGCTGCCCG
  GGTCGCATGCTGAGCCCCCTGTCCCGGCACAGGGCGAGGCCCCAGGAGAGCAGGCTCGGGACGAGCGCTC
  CGACAGCCGGGCCCAGGCGGTGTCCGAGGACGCGGGAGGAAACGAGGGCAGAGCGGCCGAGGCCGAACCC
  CGGGCGCTGGAGAATGGCGACGCGGACGAGCCCTCCTTCAGCGACCCCGAGGACTTCGTGGACGACGTGA
  GCGAGGAAGAATTACTGGGAGATGTACTCAAAGATCGGCCCCAGGAAGCAGATGGAATCGATTCGGTGAT
  TGTAGTGGACAATGTCCCTCAGGTGGGACCCGACCGACTTGAGAAACTCAAAAATGTCATCCACAAGATC
  TTTTCCAAGTTTGGGAAAATCACAAATGATTTTTATCCTGAAGAGGATGGGAAGACAAAAGGGTATATTT
  TCCTGGAGTACGCGTCCCCTGCCCACGCTGTGGATGCTGTGAAGAACGCCGACGGCTACAAGCTTGACAA
  GCAGCACACATTCCGGGTCAACCTCTTTACGGATTTTGACAAGTATATGACGATCAGTGACGAGTGGGAT
  ATTCCAGAGAAACAGCCTTTCAAAGACCTGGGGAACTTACGTTACTGGCTTGAAGAGGCAGAATGCAGAG
  ATCAGTACAGTGTGATTTTTGAGAGTGGAGACCGCACTTCCATATTCTGGAATGACGTAAAAGACCCTGT
  CTCAATTGAAGAAAGAGCGAGATGGACAGAGACGTATGTGCGTTGGTCTCCTAAGGGCACCTACCTGGCT
  ACCTTTCATCAAAGAGGCATTGCTCTATGGGGGGGAGAGAAATTCAAGCAAATTCAGAGATTCAGCCACC
  AAGGGGTTCAGCTTATTGACTTCTCACCTTGTGAAAGGTACCTGGTGACCTTTAGCCCCCTGATGGACAC
  GCAGGATGACCCTCAGGCCATAATCATCTGGGACATCCTTACGGGGCACAAGAAGAGGGGTTTTCACTGT
  GAGAGCTCAGCCCATTGGCCTATTTTTAAGTGGAGCCATGATGGCAAATTCTTTGCCAGAATGACCCTGG
  ATACGCTTAGCATCTATGAAACTCCTTCTATGGGTCTTTTGGACAAGAAGAGTTTGAAGATCTCTGGGAT
  AAAAGACTTTTCTTGGTCTCCTGGTGGTAACATAATCGCCTTCTGGGTGCCTGAAGACAAAGATATTCCA
  GCCAGGGTAACCCTGATGCAGCTCCCTACCAGGCAAGAGATCCGAGTGAGGAACCTGTTCAATGTGGTGG
  ACTGCAAGCTCCATTGGCAGAAGAACGGAGACTACTTGTGTGTGAAAGTAGATAGGACTCCGAAAGGCAC
  CCAGGGTGTTGTCACAAATTTTGAAATTTTCCGAATGAGGGAGAAACAGGTACCTGTGGATGTGGTCGAG
  ATGAAAGAAACCATCATAGCCTTTGCCTGGGAACCAAATGGAAGTAAGTTTGCTGTGCTGCACGGAGAGG
  CTCCGCGGATATCTGTGTCTTTCTACCACGTCAAAAACAACGGGAAGATTGAACTCATCAAGATGTTCGA
  CAAGCAGCAGGCGAACACCATCTTCTGGAGCCCCCAAGGACAGTTCGTGGTGTTGGCGGGCCTGAGGAGT
  ATGAACGGTGCCTTAGCGTTTGTGGACACTTCGGACTGCACGGTCATGAACATCGCAGAGCACTACATGG
  CTTCCGACGTCGAATGGGATCCTACTGGGCGCTACGTCGTCACCTCTGTGTCCTGGTGGAGCCATAAGGT
  GGACAACGCGTACTGGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTTCTGC
  CAGCTGCTGTGGCGGCCCCGGCCTCCCACACTCCTGAGCCAGGAACAGATCAAGCAAATTAAAAAGGATC
  TGAAGAAATACTCTAAGATCTTTGAACAGAAGGATCGTTTGAGTCAGTCCAAAGCCTCAAAGGAATTGGT
  GGAGAGAAGGCGCACCATGATGGAAGATTTCCGGAAGTACCGGAAAATGGCCCAGGAGCTCTATATGGAG
  CAGAAAAACGAGCGCCTGGAGTTGCGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGACGACT
  GGGAAGAGGAGACCATTGAGTTCTTCGTCACTGAAGAAATCATTCCCCTCGGGAATCAGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211093 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPEPEVRTEP
  AAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEP
  RALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKI
  FSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWD
  IPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLA
  TFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHC
  ESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIP
  ARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVE
  MKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRS
  MNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFC
  QLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYME
  QKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003751
• ORF Size: 2442 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003751.2, NM_003751.3, NP_003742.2
• RefSeq Size: 3009
• RefSeq ORF: 2445
• Locus ID: 8662
• Domains: RRM
• MW: 92.5 kDa