ASC2 (PYDC1) (NM_152901) Human Tagged ORF Clone

CAT#: RC211240

PYDC1 (Myc-DDK-tagged)-Human PYD (pyrin domain) containing 1 (PYDC1)

Product Images

Western blot validation of overexpression lysate (Cat# LY407263) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211240 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PYDC1 protein (Cat# TP311240). The protein was produced from HEK293T cells transfected with PYDC1 cDNA clone (Cat# RC211240) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PYDC1
• Synonyms: ASC2; cPOP1; POP1; PYC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211240 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAACGAAGCGCGAGGCCATCCTGAAGGTGCTGGAGAACCTGACACCGGAGGAGCTCAAGAAGTTCA
  AGATGAAGCTGGGGACGGTGCCGCTGCGCGAGGGCTTTGAGCGCATCCCGCGGGGCGCGCTCGGGCAGCT
  AGATATCGTGGACCTCACCGACAAGCTGGTCGCCTCCTACTACGAGGACTACGCAGCCGAGCTCGTCGTG
  GCCGTGCTGCGCGACATGCGCATGTTGGAGGAGGCCGCACGGCTGCAGCGGGCTGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211240 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTKREAILKVLENLTPEELKKFKMKLGTVPLREGFERIPRGALGQLDIVDLTDKLVASYYEDYAAELVV
  AVLRDMRMLEEAARLQRAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152901
• ORF Size: 267 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152901.1, NM_152901.2, NM_152901.3, NP_690865.1
• RefSeq Size: 582 bp
• RefSeq ORF: 270 bp
• Locus ID: 260434
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 10.1 kDa
• Gene Summary: Associates with PYCARD/ASC and modulates its ability to collaborate with MEFV/pyrin and NLRP3/cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS. [UniProtKB/Swiss-Prot Function]