Acetylcholinesterase (ACHE) (NM_015831) Human Tagged ORF Clone
CAT#: RC211249L4
- LentiORF®
Lenti-ORF clone of ACHE (mGFP-tagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5
"NM_015831" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | ACHE |
Synonyms | ACEE; ARACHE; N-ACHE; YT |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211249).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_015831 |
ORF Size | 1851 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015831.2, NP_056646.1 |
RefSeq Size | 2978 bp |
RefSeq ORF | 1854 bp |
Locus ID | 43 |
Cytogenetics | 7q22.1 |
Protein Families | Druggable Genome |
Protein Pathways | Glycerophospholipid metabolism |
MW | 67.38 kDa |
Gene Summary | 'Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304353 | ACHE (untagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5 |
USD 760.00 |
|
RC211249 | ACHE (Myc-DDK-tagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5 |
USD 420.00 |
|
RG211249 | ACHE (GFP-tagged) - Human acetylcholinesterase (ACHE), transcript variant E4-E5 |
USD 460.00 |
|
RC211249L3 | Lenti-ORF clone of ACHE (Myc-DDK-tagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5 |
USD 620.00 |
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