Acetylcholinesterase (ACHE) (NM_015831) Human Untagged Clone

CAT#: SC304353

ACHE (untagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5


  "NM_015831" in other vectors (4)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ACHE
Synonyms ACEE; ARACHE; N-ACHE; YT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_015831 edited
GAACATCGGCCGCCTCCAGCTCCCGGCGCGGCCCGGCCCGGCCCGGCTCGGCCGCCTCAG
CAGACGCCGCCTGCCCTGCAGCCATGAGGCCCCCGCAGTGTCTGCTGCACACGCCTTCCC
TGGCTTCCCCACTCCTTCTCCTCCTCCTCTGGCTCCTGGGTGGAGGAGTGGGGGCTGAGG
GCCGGGAGGATGCAGAGCTGCTGGTGACGGTGCGTGGGGGCCGGCTGCGGGGCATTCGCC
TGAAGACCCCCGGGGGCCCTGTCTCTGCTTTCCTGGGCATCCCCTTTGCGGAGCCACCCA
TGGGACCCCGTCGCTTTCTGCCACCGGAGCCCAAGCAGCCTTGGTCAGGGGTGGTAGACG
CTACAACCTTCCAGAGTGTCTGCTACCAATATGTGGACACCCTATACCCAGGTTTTGAGG
GCACCGAGATGTGGAACCCCAACCGTGAGCTGAGCGAGGACTGCCTGTACCTCAACGTGT
GGACACCATACCCCCGGCCTACATCCCCCACCCCTGTCCTCGTCTGGATCTATGGGGGTG
GCTTCTACAGTGGGGCCTCCTCCTTGGACGTGTACGATGGCCGCTTCTTGGTACAGGCCG
AGAGGACTGTGCTGGTGTCCATGAACTACCGGGTGGGAGCCTTTGGCTTCCTGGCCCTGC
CGGGGAGCCGAGAGGCCCCGGGCAATGTGGGTCTCCTGGATCAGAGGCTGGCCCTGCAGT
GGGTGCAGGAGAACGTGGCAGCCTTCGGGGGTGACCCGACATCAGTGACGCTGTTTGGGG
AGAGCGCGGGAGCCGCCTCGGTGGGCATGCACCTGCTGTCCCCGCCCAGCCGGGGCCTGT
TCCACAGGGCCGTGCTGCAGAGCGGTGCCCCCAATGGACCCTGGGCCACGGTGGGCATGG
GAGAGGCCCGTCGCAGGGCCACGCAGCTGGCCCACCTTGTGGGCTGTCCTCCAGGCGGCA
CTGGTGGGAATGACACAGAGCTGGTAGCCTGCCTTCGGACACGACCAGCGCAGGTCCTGG
TGAACCACGAATGGCACGTGCTGCCTCAAGAAAGCGTCTTCCGGTTCTCCTTCGTGCCTG
TGGTAGATGGAGACTTCCTCAGTGACACCCCAGAGGCCCTCATCAACGCGGGAGACTTCC
ACGGCCTGCAGGTGCTGGTGGGTGTGGTGAAGGATGAGGGCTCGTATTTTCTGGTTTACG
GGGCCCCAGGCTTCAGCAAAGACAACGAGTCTCTCATCAGCCGGGCCGAGTTCCTGGCCG
GGGTGCGGGTCGGGGTTCCCCAGGTAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATT
ACACAGACTGGCTGCATCCCGAGGACCCGGCACGCCTGAGGGAGGCCCTGAGCGATGTGG
TGGGCGACCACAATGTCGTGTGCCCCGTGGCCCAGCTGGCTGGGCGACTGGCTGCCCAGG
GTGCCCGGGTCTACGCCTACGTCTTTGAACACCGTGCTTCCACGCTCTCCTGGCCCCTGT
GGATGGGGGTGCCCCACGGCTACGAGATCGAGTTCATCTTTGGGATCCCCCTGGACCCCT
CTCGAAACTACACGGCAGAGGAGAAAATCTTCGCCCAGCGACTGATGCGATACTGGGCCA
ACTTTGCCCGCACAGGGGATCCCAATGAGCCCCGAGACCCCAAGGCCCCACAATGGCCCC
CGTACACGGCGGGGGCTCAGCAGTACGTTAGTCTGGACCTGCGGCCGCTGGAGGTGCGGC
GGGGGCTGCGCGCCCAGGCCTGCGCCTTCTGGAACCGCTTCCTCCCCAAATTGCTCAGCG
CCACCGCCTCGGAGGCTCCCAGCACCTGCCCAGGCTTCACCCATGGGGAGGCTGCTCCGA
GGCCCGGCCTCCCCCTGCCCCTCCTCCTCCTCCACCAGCTTCTCCTCCTCTTCCTCTCCC
ACCTCCGGCGGCTGTGAACACGGCCTCTTCCCCTACGGCCACAGGG
Restriction Sites Please inquire     
ACCN NM_015831
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_015831.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015831.1, NP_056646.1
RefSeq Size 2909 bp
RefSeq ORF 1854 bp
Locus ID 43
Cytogenetics 7q22.1
Protein Families Druggable Genome
Protein Pathways Glycerophospholipid metabolism
Gene Summary 'Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]'
Transcript Variant: The splice variant E4-E5 is primarily expressed in erythroid tissues, and appears to encode the PI-linked form of acetylcholinesterase. It results from tissue-specific alternative splicing of exon 4 to exon 5.

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