ADCY8 (NM_001115) Human Tagged ORF Clone

CAT#: RC211815

ADCY8 (Myc-DDK-tagged)-Human adenylate cyclase 8 (brain) (ADCY8)

Product Images

Western blot validation of overexpression lysate (Cat# LY420131) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211815 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCY8
• Synonyms: AC8; ADCY3; HBAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211815 representing NM_001115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTCCGATGTGCGCTGCCTTACAGGCAGCGAGGAACTCTACACCATCCACCCGACGCCCCCGG
  CCGGCGACGGCAGGAGCGCCTCCCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGACACATCACGGA
  GCAGCGCTTCATTCACGGGCACCGGGGAGGCAGCGGCAGCGGGAGTGGAGGCTCGGGCAAAGCCTCGGAC
  CCTGCGGGCGGCGGCCCCAACCACCACACGCCGCAGCTGTCAGGCGACTCGGCGCTGCCCCTCTACTCGC
  TGGGCCCGGGAGAGCGAGCGCACAGCACCTGCGGCACCAAAGTCTTCCCGGAACGCAGCGGGAGCGGCAG
  TGCCAGCGGCAGCGGAGGCGGGGGCGACCTGGGCTTCCTGCACCTTGACTGTGCCCCTAGCAACTCGGAT
  TTCTTTCTTAATGGGGGCTATAGCTACCGAGGGGTCATTTTCCCCACCCTGCGCAACTCCTTCAAATCTC
  GGGATTTGGAACGCCTCTACCAGCGCTATTTCTTGGGCCAAAGGCGCAAATCGGAAGTGGTGATGAACGT
  GCTGGACGTGCTGACCAAACTCACTCTCTTGGTCCTACACTTGAGCCTGGCCTCGGCCCCCATGGACCCG
  CTCAAGGGCATCCTGCTGGGCTTCTTCACCGGCATTGAGGTAGTGATCTGCGCCCTGGTGGTGGTCAGGA
  AGGACACCACCTCCCACACGTACCTGCAGTACAGCGGCGTGGTCACCTGGGTGGCCATGACCACCCAGAT
  CCTGGCAGCAGGCCTCGGCTACGGGCTCCTGGGCGACGGCATAGGCTACGTGCTCTTCACGCTCTTCGCC
  ACCTACAGTATGCTGCCGCTGCCGCTCACCTGGGCCATCCTGGCCGGCCTGGGCACCTCGCTGCTGCAGG
  TCATCCTCCAAGTGGTCATACCCCGGCTGGCGGTCATTTCCATCAACCAGGTTGTGGCCCAGGCAGTGCT
  ATTCATGTGTATGAACACAGCTGGAATCTTCATCAGTTACCTGTCAGACCGTGCCCAGCGCCAAGCTTTC
  CTGGAGACTCGGAGGTGTGTGGAGGCCAGGCTGCGCCTGGAGACAGAGAACCAAAGACAGGAGCGGCTCG
  TGCTTTCTGTGCTCCCCCGGTTTGTTGTCCTGGAAATGATCAACGACATGACCAATGTGGAAGATGAGCA
  CCTGCAGCACCAGTTCCATCGGATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTT
  AAAGGATTTACCAACCTCTCCACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAGCTCTTTG
  CCAGATTTGATCGACTGGCCCATGAGCATCACTGCCTTCGTATTAAAATCCTGGGGGACTGCTACTACTG
  CGTGTCTGGACTTCCTGAGCCCCGCCAGGACCATGCCCACTGCTGTGTTGAAATGGGTCTCAGCATGATC
  AAAACCATCAGGTATGTGCGGTCAAGGACAAAACACGATGTTGACATGAGGATTGGAATCCACTCCGGCT
  CGGTGCTGTGCGGTGTTTTGGGACTAAGGAAGTGGCAGTTTGATGTCTGGTCTTGGGATGTGGATATTGC
  AAACAAACTCGAATCTGGAGGAATCCCTGGGAGGATTCACATTTCCAAAGCCACGCTGGACTGTCTCAAC
  GGTGACTATAACGTGGAAGAGGGCCATGGTAAAGAGAGGAATGAATTCCTGAGGAAGCATAATATCGAAA
  CTTACTTAATTAAGCAGCCTGAGGACAGTCTGCTGTCCTTGCCTGAAGATATCGTCAAGGAGTCAGTGAG
  CTCCTCAGACCGGAGAAACAGTGGGGCCACATTCACTGAAGGATCCTGGAGCCCTGAACTGCCCTTTGAT
  AATATCGTGGGGAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATC
  TTGCACAAGCTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAACATACCATCGACTT
  GCGGAGTGGCGATAAATTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTG
  GAGCACAAGTATTCTCAAATGAGGGATGAAGTGTTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTC
  TATTTATCACGGCAATACAAAGTTTGCTTCCTTCTTCAAGAGTGATGCCAATGACCATCCAGTTCTCCAT
  TCTGATTATGCTGCACTCGGCTCTGGTCCTCATCACCACAGCAGAGGATTATAAATGTTTGCCCCTCATC
  CTCCGGAAAACTTGCTGTTGGATTAATGAGACCTATTTGGCCCGGAACGTCATCATCTTTGCATCCATTT
  TGATTAATTTCCTGGGTGCCATCTTAAATATCCTGTGGTGTGATTTTGACAAGTCGATACCCTTGAAGAA
  CCTGACTTTCAATTCCTCAGCTGTGTTTACAGATATCTGCTCCTACCCAGAGTACTTTGTCTTCACGGGG
  GTGTTGGCCATGGTGACCTGTGCAGTTTTCCTCCGGCTGAACTCCGTCCTGAAGCTGGCAGTGCTGCTGA
  TCATGATTGCCATCTATGCCCTGCTCACTGAGACCGTCTACGTAGGCCTCTTTCTGCGTTATGACAACCT
  CAACCACAGTGGAGAAGATTTCCTGGGGACCAAGGAGGTATCACTGCTACTGATGGCCATGTTCCTCCTG
  GCTGTGTTCTACCATGGACAGCAGCTGGAGTACACAGCCCGCCTGGACTTCCTTTGGCGAGTACAGGCCA
  AAGAGGAGATCAATGAGATGAAGGAGCTGAGGGAACACAATGAGAACATGCTCCGGAATATCTTACCCAG
  CCATGTGGCCCGCCATTTCCTAGAGAAGGACCGAGACAATGAGGAGCTGTATTCTCAATCCTATGATGCT
  GTTGGGGTGATGTTTGCCTCCATCCCAGGATTTGCGGACTTTTACTCTCAGACTGAAATGAATAACCAGG
  GAGTGGAATGCCTGCGCTTGCTCAATGAGATCATTGCTGACTTCGATGAGTTGCTTGGTGAAGACCGATT
  TCAAGACATTGAAAAGATTAAGACCATTGGCAGCACCTACATGGCCGTGTCAGGCCTGTCACCTGAAAAA
  CAGCAATGTGAAGACAAGTGGGGACATTTGTGTGCTCTGGCTGACTTCTCACTCGCCCTGACAGAAAGCA
  TACAGGAGATCAACAAGCATTCATTCAACAATTTTGAACTCCGGATTGGCATCAGCCACGGCTCAGTGGT
  AGCTGGCGTTATCGGCGCTAAGAAACCACAGTATGACATTTGGGGCAAAACTGTGAACCTGGCAAGCCGA
  ATGGACAGCACGGGGGTTAGTGGCCGGATCCAAGTCCCAGAGGAGACCTATCTCATCCTGAAGGACCAGG
  GCTTTGCCTTTGATTACCGAGGGGAGATCTATGTGAAGGGTATCAGTGAACAGGAAGGAAAAATCAAAAC
  GTACTTTCTTCTGGGAAGAGTCCAACCCAACCCATTCATCTTGCCCCCAAGAAGACTGCCTGGGCAGTAC
  TCCCTGGCCGCGGTTGTCCTGGGACTTGTCCAGTCCCTCAATAGGCAAAGGCAGAAGCAGCTACTCAATG
  AGAACAACAACACAGGAATCATCAAGGGTCATTACAACCGGCGGACTTTGTTGTCACCCAGCGGCACAGA
  GCCTGGAGCCCAGGCTGAAGGCACCGACAAATCTGATTTGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211815 representing NM_001115
  Red=Cloning site Green=Tags(s)
  
  MELSDVRCLTGSEELYTIHPTPPAGDGRSASRPQRLLWQTAVRHITEQRFIHGHRGGSGSGSGGSGKASD
  PAGGGPNHHTPQLSGDSALPLYSLGPGERAHSTCGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSD
  FFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDP
  LKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFA
  TYSMLPLPLTWAILAGLGTSLLQVILQVVIPRLAVISINQVVAQAVLFMCMNTAGIFISYLSDRAQRQAF
  LETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADV
  KGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMI
  KTIRYVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLN
  GDYNVEEGHGKERNEFLRKHNIETYLIKQPEDSLLSLPEDIVKESVSSSDRRNSGATFTEGSWSPELPFD
  NIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSL
  EHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRVMPMTIQFSILIMLHSALVLITTAEDYKCLPLI
  LRKTCCWINETYLARNVIIFASILINFLGAILNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTG
  VLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETVYVGLFLRYDNLNHSGEDFLGTKEVSLLLMAMFLL
  AVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDA
  VGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEK
  QQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASR
  MDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQY
  SLAAVVLGLVQSLNRQRQKQLLNENNNTGIIKGHYNRRTLLSPSGTEPGAQAEGTDKSDLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115
• ORF Size: 3753 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115.1, NM_001115.2, NP_001106.1
• RefSeq Size: 6005 bp
• RefSeq ORF: 3756 bp
• Locus ID: 114
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction, Vascular smooth muscle contraction
• MW: 139.9 kDa
• Gene Summary: Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul 2008]