gamma Adducin (ADD3) (NM_001121) Human Tagged ORF Clone

CAT#: RC211957

ADD3 (Myc-DDK-tagged)-Human adducin 3 (gamma) (ADD3), transcript variant 3

Product Images

Coomassie blue staining of purified ADD3 protein (Cat# TP311957). The protein was produced from HEK293T cells transfected with ADD3 cDNA clone (Cat# RC211957) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADD3
• Synonyms: ADDL; CPSQ3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211957 representing NM_001121
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGAT
  ATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACA
  AGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGAC
  TTGGAATGCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGA
  TTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCAC
  ACCTATCAATGACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA
  CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTATATCTCAGTAA
  GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAAGCTACAGCCTC
  CAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGACCAGGGAAGTACCAATTTGAAAATTGACCAT
  ACAGGATTCAGTCCCCATGCTGCAATCTATTCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATA
  CCCTTGCAACAGCAGCTGTATCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCT
  GGGAGATGTTGCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG
  GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAG
  AGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTGCAGGCCCTAGCAGGTGC
  AGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAGTATAAAGCTTTCACTTACACTGTAGCAGCG
  TCTGGTGGAGGAGGTGTGAATATGGGTTCCCATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGC
  TTATGAGGACTCTGGACAACTTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAA
  GCCTAGGCACAAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG
  CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTGAACTCACCAA
  ATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACCAGTCCCCGAACCAAAATCAC
  GTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGAACACCTATCAAAATTGAAGATCCAAATCAG
  TTTGTTCCTTTAAACACAAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGAT
  ATGACTTGAAAACAGCAGGACCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTAC
  TATGCAATTTGAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA
  GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGAAAACCATGAGCTGTTTT
  CCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAATGGCAAGGATGATATGCATGATGTTGA
  AGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAACCATAGAAAACATCGAGATTACTATT
  AAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTCCCCTTCAAAATCGCCATCCAAGAAAA
  AGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211957 representing NM_001121
  Red=Cloning site Green=Tags(s)
  
  MSSDASQGVITTPPPPSMPHKERYFDRINENDPEYIRERNMSPDLRQDFNMMEQRKRVTQILQSPAFRED
  LECLIQEQMKKGHNPTGLLALQQIADYIMANSFSGFSSPPLSLGMVTPINDLPGADTSSYVKGEKLTRCK
  LASLYRLVDLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASNLVKVNIIGEVVDQGSTNLKIDH
  TGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLLLGDVAYYDYQGSLEEQEERIQLQK
  VLGPSCKVLVLRNHGVVALGETLEEAFHYIFNVQLACEIQVQALAGAGGVDNLHVLDFQKYKAFTYTVAA
  SGGGGVNMGSHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTV
  PLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQ
  FVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEG
  ELEEYKRTIERKQQGLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITI
  KSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001121
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001121.2, NM_001121.3, NP_001112.2
• RefSeq Size: 4358 bp
• RefSeq ORF: 2025 bp
• Locus ID: 120
• Cytogenetics: 10q25.1-q25.2
• MW: 75.5 kDa
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]'