gamma Adducin (ADD3) (NM_001121) Human Untagged Clone

CAT#: SC302974

ADD3 (untagged)-Human adducin 3 (gamma) (ADD3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD3
• Synonyms: ADDL; CPSQ3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001121, the custom clone sequence may differ by one or more nucleotides
  ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCAC
  AAAGAGAGATATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAAC
  ATGTCTCCTGATCTACGACAAGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAG
  ATCCTGCAAAGTCCTGCCTTTCGGGAAGACTTGGAATGCCTTATTCAAGAACAGATGAAG
  AAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGATTGCAGATTACATCATGGCC
  AATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCACACCTATCAAT
  GACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA
  CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTAT
  ATCTCAGTAAGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCT
  TTTTCTGAAGCTACAGCCTCCAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGAC
  CAGGGAAGTACCAATTTGAAAATTGACCATACAGGATTCAGTCCCCATGCTGCAATCTAT
  TCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATACCCTTGCAACAGCAGCTGTA
  TCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCTGGGAGATGTT
  GCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG
  GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGA
  GAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAG
  GTGCAGGCCCTAGCAGGTGCAGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAG
  TATAAAGCTTTCACTTACACTGTAGCAGCGTCTGGTGGAGGAGGTGTGAATATGGGTTCC
  CATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGCTTATGAGGACTCTGGACAAC
  TTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAAGCCTAGGCAC
  AAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG
  CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTG
  AACTCACCAAATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACC
  AGTCCCCGAACCAAAATCACGTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGA
  ACACCTATCAAAATTGAAGATCCAAATCAGTTTGTTCCTTTAAACACAAACCCGAATGAG
  GTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGATATGACTTGAAAACAGCAGGA
  CCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTACTATGCAATTT
  GAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA
  GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGAAAACCAT
  GAGCTGTTTTCCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAATGGCAAG
  GATGATATGCATGATGTTGAAGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGT
  ACAACCATAGAAAACATCGAGATTACTATTAAGTCTCCAGAGAAAATCGAAGAAGTCCTG
  TCACCTGAAGGCTCCCCTTCAAAATCGCCATCCAAGAAAAAGAAGAAATTCCGCACTCCT
  TCTTTTCTGAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001121
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001121.2, NP_001112.2
• RefSeq Size: 4358 bp
• RefSeq ORF: 2025 bp
• Locus ID: 120
• Cytogenetics: 10q25.1-q25.2
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 1.The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b).