PPAP2C (NM_177543) Human Tagged ORF Clone

CAT#: RC212478

PPAP2C (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPAP2C
• Synonyms: LPP2; PAP-2c; PAP2-g; PPAP2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212478 representing NM_177543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTCGCGAGAGGCCCGGGGAGCCGGGGCCAGCATCCCCCGCCCCGGCAGCAGGAAGTCTGTGCGG
  AGGGGCCGCGCGCGCGCCTCCATCCCGCCCCGCCTGGCCTGGGAGCCTCCCTGCCCTTCGCTATCCTGAC
  GCTGGTGAACGCCCCGTACAAGCGAGGATTTTACTGCGGGGATGACTCCATCCGGTACCCCTACCGTCCA
  GATACCATCACCCACGGGCTCATGGCTGGGGTCACCATCACGGCCACCGTCATCCTTGTCTCGGCCGGGG
  AAGCCTACCTGGTGTACACAGACCGGCTCTATTCTCGCTCGGACTTCAACAACTACGTGGCTGCTGTATA
  CAAGGTGCTGGGGACCTTCCTGTTTGGGGCTGCCGTGAGCCAGTCTCTGACAGACCTGGCCAAGTACATG
  ATTGGGCGTCTGAGGCCCAACTTCCTAGCCGTCTGCGACCCCGACTGGAGCCGGGTCAACTGCTCGGTCT
  ATGTGCAGCTGGAGAAGGTGTGCAGGGGAAACCCTGCTGATGTCACCGAGGCCAGGTTGTCTTTCTACTC
  GGGACACTCTTCCTTTGGGATGTACTGCATGGTGTTCTTGGCGCTGTATGTGCAGGCACGACTCTGTTGG
  AAGTGGGCACGGCTGCTGCGACCCACAGTCCAGTTCTTCCTGGTGGCCTTTGCCCTCTACGTGGGCTACA
  CCCGCGTGTCTGATTACAAACACCACTGGAGCGATGTCCTTGTTGGCCTCCTGCAGGGGGCACTGGTGGC
  TGCCCTCACTGTCTGCTACATCTCAGACTTCTTCAAAGCCCGACCCCCACAGCACTGTCTGAAGGAGGAG
  GAGCTGGAACGGAAGCCCAGCCTGTCACTGACGTTGACCCTGGGCGAGGCTGACCACAACCACTATGGAT
  ACCCGCACTCCTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212478 representing NM_177543
  Red=Cloning site Green=Tags(s)
  
  MGVARGPGSRGQHPPPRQQEVCAEGPRARLHPAPPGLGASLPFAILTLVNAPYKRGFYCGDDSIRYPYRP
  DTITHGLMAGVTITATVILVSAGEAYLVYTDRLYSRSDFNNYVAAVYKVLGTFLFGAAVSQSLTDLAKYM
  IGRLRPNFLAVCDPDWSRVNCSVYVQLEKVCRGNPADVTEARLSFYSGHSSFGMYCMVFLALYVQARLCW
  KWARLLRPTVQFFLVAFALYVGYTRVSDYKHHWSDVLVGLLQGALVAALTVCYISDFFKARPPQHCLKEE
  ELERKPSLSLTLTLGEADHNHYGYPHSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177543
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177543.1, NM_177543.2, NP_808211.1
• RefSeq Size: 1394
• RefSeq ORF: 930
• Locus ID: 8612
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• MW: 34.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]