MGAT5 (NM_002410) Human Tagged ORF Clone

CAT#: RC213548

MGAT5 (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGAT5
• Synonyms: GNT-V; GNT-VA; MGAT5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213548 representing NM_002410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCTTCACTCCGTGGAAGTTGTCCTCTCAGAAGCTGGGCTTTTTCCTGGTGACTTTTGGCTTCA
  TTTGGGGTATGATGCTTCTGCACTTTACCATCCAGCAGCGAACTCAGCCTGAAAGCAGCTCCATGCTGCG
  CGAGCAGATCCTGGACCTCAGCAAAAGGTACATCAAGGCACTGGCAGAAGAAAACAGGAATGTGGTGGAT
  GGGCCATACGCTGGAGTCATGACAGCTTATGATCTGAAGAAAACCCTTGCTGTGTTATTAGATAACATTT
  TGCAGCGCATTGGCAAGTTGGAGTCGAAGGTGGACAATCTTGTTGTCAATGGCACCGGAACAAACTCAAC
  CAACTCCACTACAGCTGTTCCCAGCTTGGTTGCACTTGAGAAAATTAATGTGGCAGATATCATTAACGGA
  GCTCAAGAAAAATGTGTATTGCCTCCTATGGACGGCTACCCTCACTGTGAGGGAAAGATCAAGTGGATGA
  AAGACATGTGGCGTTCAGATCCCTGCTACGCAGACTATGGAGTGGATGGATCCACCTGCTCTTTTTTTAT
  TTACCTCAGTGAGGTTGAAAATTGGTGTCCTCATTTACCTTGGAGAGCAAAAAATCCCTACGAAGAAGCT
  GATCATAATTCATTGGCGGAAATTCGTACAGATTTTAATATTCTCTACAGTATGATGAAAAAGCATGAAG
  AATTCCGGTGGATGAGACTACGGATCCGGCGAATGGCTGACGCATGGATCCAAGCAATCAAGTCCCTGGC
  AGAAAAGCAGAACCTTGAAAAGAGAAAGCGGAAGAAAGTCCTCGTTCACCTGGGACTCCTGACCAAGGAA
  TCTGGATTTAAGATTGCAGAGACAGCTTTCAGTGGTGGCCCTCTTGGTGAATTAGTTCAATGGAGTGATT
  TAATTACATCTCTGTACTTACTGGGCCATGACATTAGGATTTCAGCTTCACTGGCTGAGCTCAAGGAAAT
  CATGAAGAAGGTTGTAGGAAACCGATCTGGCTGCCCAACTGTAGGAGACAGAATTGTTGAGCTCATTTAC
  ATTGATATTGTAGGACTTGCTCAATTCAAGAAAACTCTTGGACCATCCTGGGTTCATTACCAGTGCATGC
  TCCGAGTCCTTGATTCATTTGGTACTGAACCCGAATTTAATCATGCAAATTATGCCCAATCGAAAGGCCA
  CAAGACCCCTTGGGGAAAATGGAATCTGAACCCTCAGCAGTTTTATACCATGTTCCCTCATACCCCAGAC
  AACAGCTTTCTGGGGTTTGTGGTTGAGCAGCACCTGAACTCCAGTGATATCCACCACATTAATGAAATCA
  AAAGGCAGAACCAGTCCCTTGTGTATGGCAAAGTGGATAGCTTCTGGAAGAATAAGAAGATCTACTTGGA
  CATTATTCACACATACATGGAAGTGCATGCAACTGTTTATGGCTCCAGCACAAAGAATATTCCCAGTTAC
  GTGAAAAACCATGGTATCCTCAGTGGACGGGACCTGCAGTTCCTTCTTCGAGAAACCAAGTTGTTTGTTG
  GACTTGGGTTCCCTTACGAGGGCCCAGCTCCCCTGGAAGCTATCGCAAATGGATGTGCTTTTCTGAATCC
  CAAGTTCAACCCACCCAAAAGCAGCAAAAACACAGACTTTTTCATTGGCAAGCCAACTCTGAGAGAGCTG
  ACATCCCAGCATCCTTACGCTGAAGTTTTCATCGGGCGGCCACATGTGTGGACTGTTGACCTCAACAATC
  AGGAGGAAGTAGAGGATGCAGTGAAAGCAATTTTAAATCAGAAGATTGAGCCATACATGCCATATGAATT
  TACGTGCGAGGGGATGCTACAGAGAATCAATGCTTTCATTGAAAAACAGGACTTCTGCCATGGGCAAGTG
  ATGTGGCCACCCCTCAGCGCCCTACAGGTCAAGCTTGCTGAGCCCGGGCAGTCCTGCAAGCAGGTGTGCC
  AGGAGAGCCAGCTCATCTGCGAGCCTTCTTTCTTCCAGCACCTCAACAAGGACAAGGACATGCTGAAGTA
  CAAGGTGACCTGCCAAAGCTCAGAGCTGGCCAAGGACATCCTGGTGCCCTCCTTTGACCCTAAGAATAAG
  CACTGTGTGTTTCAAGGTGACCTCCTGCTCTTCAGCTGTGCAGGCGCCCACCCCAGGCACCAGAGGGTCT
  GCCCCTGCCGGGACTTCATCAAGGGCCAGGTGGCTCTCTGCAAAGACTGCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213548 representing NM_002410
  Red=Cloning site Green=Tags(s)
  
  MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKALAEENRNVVD
  GPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVVNGTGTNSTNSTTAVPSLVALEKINVADIING
  AQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGVDGSTCSFFIYLSEVENWCPHLPWRAKNPYEEA
  DHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKE
  SGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIY
  IDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPD
  NSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSY
  VKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLREL
  TSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQV
  MWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNK
  HCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002410
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002410.1, NM_002410.2, NM_002410.3, NM_002410.4, NP_002401.1
• RefSeq Size: 5078 bp
• RefSeq ORF: 2226 bp
• Locus ID: 4249
• Cytogenetics: 2q21.2-q21.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 84.4 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]'