MGAT5 (NM_002410) Human Untagged Clone

CAT#: SC314591

MGAT5 (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT5
• Synonyms: GNT-V; GNT-VA; MGAT5A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002410 edited
  TTCTACGCGTTAAGAGCCAAGGACAGGTGAAGTTGCCAGAGAGCAATGGCTCTCTTCACT
  CCGTGGAAGTTGTCCTCTCAGAAGCCGGGCTTTTTCCTGGTGACTTTTGGCTTCATTTGG
  GGTATGATGCTTCTGCACTTTACCATCCAGCAGCGAACTCAGCCTGAAAGCAGCTCCATG
  CTGCGCGAGCAGATCCTGGACCTCAGCAAAAGGTACATCAAGGCACTGGCAGAAGAAAAC
  AGGAATGTGGTGGATGGGCCATACGCTGGAGTCATGACAGCTTATGATCTGAAGAAAACC
  CTTGCTGTGTTATTAGATAACATTTTGCAGCGCATTGGCAAGTTGGAGTCGAAGGTGGAC
  AATCTTGTTGTCAATGGCACCGGAACAAACTCAACCAACTCCACTACAGCTGTTCCCAGC
  TTGGTTGCACTTGAGAAAATTAATGTGGCAGATATCATTAACGGAGCTCAAGAAAAATGT
  GTATTGCCTCCTATGGACGGCTACCCTCACTGTGAGGGAAAGATCAAGTGGATGAAAGAC
  ATGTGGCGTTCAGATCCCTGCTACGCAGACTATGGAGTGGATGGATCCACCTGCTCTTTT
  TTTATTTACCTCAGTGAGGTTGAAAATTGGTGTCCTCATTTACCTTGGAGAGCAAAAAAT
  CCCTACGAAGAAGCTGATCATAATTCATTGGCGGAAATTCGTACAGATTTTAATATTCTC
  TACAGTATGATGAAAAAGCATGAAGAATTCCGGTGGATGAGACTACGGATCCGGCGAATG
  GCTGACGCATGGATCCAAGCAATCAAGTCCCTGGCAGAAAAGCAGAACCTTGAAAAGAGA
  AAGCGGAAGAAAGTCCTCGTTCACCTGGGACTCCTGACCAAGGAATCTGGATTTAAGATT
  GCAGAGACAGCTTTCAGTGGTGGCCCTCTTGGTGAATTAGTTCAATGGAGTGATTTAATT
  ACATCTCTGTACTTACTGGGCCATGACATTAGGATTTCAGCTTCACTGGCTGAGCTCAAG
  GAAATCATGAAGAAGGTTGTAGGAAACCGATCTGGCTGCCCAACTGTAGGAGACAGAATT
  GTTGAGCTCATTTACATTGATATTGTAGGACTTGCTCAATTCAAGAAAACTCTTGGACCA
  TCCTGGGTTCATTACCAGTGCATGCTCCGAGTCCTTGATTCATTTGGTACTGAACCCGAA
  TTTAATCATGCAAATTATGCCCAATCGAAAGGCCACAAGACCCCTTGGGGAAAATGGAAT
  CTGAACCCTCAGCAGTTTTATACCATGTTCCCTCATACCCCAGACAACAGCTTTCTGGGG
  TTTGTGGTTGAGCAGCACCTGAACTCCAGTGATATCCACCACATTAATGAAATCAAAAGG
  CAGAACCAGTCCCTTGTGTATGGCAAAGTGGATAGCTTCTGGAAGAATAAGAAGATCTAC
  TTGGACATTATTCACACATACATGGAAGTGCATGCAACTGTTTATGGCTCCAGCACAAAG
  AATATTCCCAGTTACGTGAAAAACCATGGTATCCTCAGTGGACGGGACCTGCAGTTCCTT
  CTTCGAGAAACCAAGTTGTTTGTTGGACTTGGGTTCCCTTACGAGGGCCCAGCTCCCCTG
  GAAGCTATCGCAAATGGATGTGCTTTTCTGAATCCCAAGTTCAACCCACCCAAAAGCAGC
  AAAAACACAGACTTTTTCATTGGCAAGCCAACTCTGAGAGAGCTGACATCCCAGCATCCT
  TACGCTGAAGTTTTCATCGGGCGGCCACATGTGTGGACTGTTGACCTCAACAATCAGGAG
  GAAGTAGAGGATGCAGTGAAAGCAATTTTAAATCAGAAGATTGAGCCATACATGCCATAT
  GAATTTACGTGCGAGGGGATGCTACAGAGAATCAATGCTTTCATTGAAAAACAGGACTTC
  TGCCATGGGCAAGTGATGTGGCCACCCCTCAGCGCCCTACAGGTCAAGCTTGCTGAGCCC
  GGGCAGTCCTGCAAGCAGGTGTGCCAGGAGAGCCAGCTCATCTGCGAGCCTTCTTTCTTC
  CAGCACCTCAACAAGGACAAGGACATGCTGAAGTACAAGGTGACCTGCCAAAGCTCAGAG
  CTGGCCAAGGACATCCTGGTGCCCTCCTTTGACCCTAAGAATAAGCACTGTGTGTTTCAA
  GGTGACCTCCTGCTCTTCAGCTGTGCAGGCGCCCACCCCAGGCACCAGAGGGTCTGCCCC
  TGCCGGGACTTCATCAAGGGCCAGGTGGCTCTCTGCAAAGACTGCCTATAGCAGCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_002410
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002410.2, NP_002401.1
• RefSeq Size: 5078 bp
• RefSeq ORF: 2226 bp
• Locus ID: 4249
• Cytogenetics: 2q21.2-q21.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]'