CHM (NM_000390) Human Tagged ORF Clone
CAT#: RC213610L4
- LentiORF®
Lenti-ORF clone of CHM (mGFP-tagged)-Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1
"NM_000390" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CHM |
Synonyms | DXS540; GGTA; HSD-32; REP-1; TCD |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC213610).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000390 |
ORF Size | 1959 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000390.2, NP_000381.1 |
RefSeq Size | 5442 bp |
RefSeq ORF | 1962 bp |
Locus ID | 1121 |
Cytogenetics | Xq21.2 |
Domains | GDI |
Protein Families | Druggable Genome |
MW | 73.5 kDa |
Gene Summary | 'This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119948 | CHM (untagged)-Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1 |
USD 1,100.00 |
|
RC213610 | CHM (Myc-DDK-tagged)-Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1 |
USD 480.00 |
|
RG213610 | CHM (GFP-tagged) - Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1 |
USD 530.00 |
|
RC213610L3 | Lenti-ORF clone of CHM (Myc-DDK-tagged)-Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1 |
USD 680.00 |
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