CHM (NM_000390) Human Untagged Clone

CAT#: SC119948

CHM (untagged)-Human choroideremia (Rab escort protein 1) (CHM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHM
• Synonyms: DXS540; GGTA; HSD-32; REP-1; TCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119948 sequence for NM_000390 edited (data generated by NextGen Sequencing)
  ATGGCGGATACTCTCCCTTCGGAGTTTGATGTGATCGTAATAGGGACGGGTTTGCCTGAA
  TCCATCATTGCAGCTGCATGTTCAAGAAGTGGCCGGAGAGTTCTGCATGTTGATTCAAGA
  AGCTACTATGGAGGAAACTGGGCCAGTTTTAGCTTTTCAGGACTATTGTCCTGGCTAAAG
  GAATACCAGGAAAACAGTGACATTGTAAGTGACAGTCCAGTGTGGCAAGACCAGATCCTT
  GAAAATGAAGAAGCCATTGCTCTTAGCAGGAAGGACAAAACTATTCAACATGTGGAAGTA
  TTTTGTTATGCCAGTCAGGATTTGCATGAAGATGTCGAAGAAGCTGGTGCACTGCAGAAA
  AATCATGCTCTTGTGACATCTGCAAACTCCACAGAAGCTGCAGATTCTGCCTTCCTGCCT
  ACGGAGGATGAGTCATTAAGCACTATGAGCTGTGAAATGCTCACAGAACAAACTCCAAGC
  AGCGATCCAGAGAATGCGCTAGAAGTAAATGGTGCTGAAGTGACAGGGGAAAAAGAAAAC
  CATTGTGATGATAAAACTTGTGTGCCATCAACTTCAGCAGAAGACATGAGTGAAAATGTG
  CCTATAGCAGAAGATACCACAGAGCAACCAAAGAAAAACAGAATTACTTACTCACAAATT
  ATTAAAGAAGGCAGGAGATTTAATATTGATTTAGTATCAAAGCTGCTGTATTCTCGAGGA
  TTACTAATTGATCTTCTAATCAAATCTAATGTTAGTCGATATGCAGAGTTTAAAAATATT
  ACCAGGATTCTTGCATTTCGAGAAGGACGAGTGGAACAGGTTCCGTGTTCCAGAGCAGAT
  GTCTTTAATAGCAAACAACTTACTATGGTAGAAAAGCGAATGCTAATGAAATTTCTTACA
  TTTTGTATGGAATATGAGAAATATCCTGATGAATATAAAGGATATGAAGAGATCACATTT
  TATGAATATTTAAAGACTCAAAAATTAACCCCCAACCTCCAATATATTGTCATGCATTCA
  ATTGCAATGACATCAGAGACAGCCAGCAGCACCATAGATGGTCTCAAAGCTACCAAAAAC
  TTTCTTCACTGTCTTGGGCGGTATGGCAACACTCCATTTTTGTTTCCTTTATATGGCCAA
  GGAGAACTCCCCCAGTGTTTCTGCAGGATGTGTGCTGTGTTTGGTGGAATTTATTGTCTT
  CGCCATTCAGTACAGTGCCTTGTAGTGGACAAAGAATCCAGAAAATGTAAAGCAATTATA
  GATCAGTTTGGTCAGAGAATAATCTCTGAGCATTTCCTCGTGGAGGACAGTTACTTTCCT
  GAGAACATGTGCTCACGTGTGCAATACAGGCAGATCTCCAGGGCAGTGCTGATTACAGAT
  AGATCTGTCCTAAAAACAGATTCAGATCAACAGATTTCCATTTTGACAGTGCCAGCAGAG
  GAACCAGGAACTTTTGCTGTTCGGGTCATTGAGTTATGTTCTTCAACGATGACATGCATG
  AAAGGCACCTATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTA
  GAATCAGTTGTGCAGAAATTGTTTGTTCCATATACTGAAATGGAGATAGAAAATGAACAA
  GTAGAAAAGCCAAGAATTCTGTGGGCTCTTTACTTCAATATGAGAGATTCGTCAGACATC
  AGCAGGAGCTGTTATAATGATTTACCATCCAACGTTTATGTCTGCTCTGGCCCAGATTGT
  GGTTTAGGAAATGATAATGCAGTCAAACAGGCTGAAACACTTTTCCAGGAAATCTGCCCC
  AATGAAGATTTCTGTCCCCCTCCACCAAATCCTGAAGACATTATCCTTGATGGAGACAGT
  TTACAGCCAGAGGCTTCAGAATCCAGTGCCATACCAGAGGCTAACTCGGAGACTTTCAAG
  GAAAGCACAAACCTTGGAAACCTAGAGGAGTCCTCTGAATAA
  
  Clone variation with respect to NM_000390.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000390 unedited
  TACTAATTTGTNAATACGCACTCTCTATAGGGNNCGGCCGCGCAATTCGGCACGAGGTCA
  AGATGGCGGATACTCTCCCTTCGGAGTTTGATGTGATCGTAATAGGGACGGGTTTGCCTG
  AATCCATCATTGCAGCTGCATGTTCAAGAAGTGGCCGGAGAGTTCTGCATGTTGATTCAA
  GAAGCTACTATGGAGGAAACTGGGCCAGTTTTAGCTTTTCAGGACTATTGTCCTGGCTAA
  AGGAATACCAGGAAAACAGTGACATTGTAAGTGACAGTCCAGTGTGGCAAGACCAGATCC
  TTGAAAATGAAGAAGCCATTGCTCTTAGCAGGAAGGACAAAACTATTCAACATGTGGAAG
  TATTTTGTTATGCCAGTCAGGATTTGCATGAAGATGTCGAAGAAGCTGGTGCACTGCAGA
  AAAATCATGCTCTTGTGACATCTGCAAACTCCACAGAAGCTGCAGATTCTGCCTTCCTGC
  CTACGGAGGATGAGTCATTAAGCACTATGAGCTGTGAAATGCTCACAGAACAAACTCCAA
  GCAGCGATCCAGAGAATGCGCTAGAAGTAAATGGTGCTGAAGTGACAGGGGAAAAAGATA
  ACCATTGTGATGATAAAACTTGTGTGCCATCAACTTCAGCAGAAGACATGAGTGAAAATG
  TGCCTATAGCAGAAGATACCACAGAGCACCCAAAGAAAACCAGAATTACTTACTCACAAA
  ATATTAAAGAAGGCAGGAGATTTAATATTGATTTGTATCAAAGCTGCTGTATTCTCGAAG
  ATTACCTAATGATCTTCTAATCAAACCTATGGTAGTCGATATGCAGAGTTTAAAACTATA
  CCAAGATCTTTGCATTTCGAAAAGAACGATTGAACAAGTTCCGCGTTCCAGAGCAGATGC
  TTTTATAGCAACCACTTACTATGCAGGAAACCGAAGCTAATGAAATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000390 unedited
  GTCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGAGACAGAGTCTTG
  CTCTGTCACCCACGCTGGAGTGCAGTGGCAAAATCTTGGCTCACTGCAACCTCCGCCTCC
  CTGGTTCAAGTGAGTCTGCTACCTCATCTTCAAAGTAGCTGGGATTACAGGTGCCCACCA
  TAACACCCAGCTAAAATTTTGTATTTTTAGTAGAGACAGGGTTTCGCCATGTTGGCCAGG
  CTGGTCTCGAACTCCTGACCTCAAGTGATCTACCCACCTCAGCTTCCCAAAGTGCTGGGA
  TTACAGGCAGCCCTAACGTTTTTATGTAGTGTATATTAGGGCTTACCAACTGTCATACAC
  AATTTGGAGTCCTTGGTGGTTGCAGGTTTACCTACCAGAAAAGGAATAAAACAATTTATA
  GAGTGTTTTCCTTAGTAATATTTCAAAACCACAATTCTACTTGTTACTATTTTTACTGGT
  CTGTAAGACTATAAAATACTACTTTAAAAAAGAAATTTTCATTTAAAAAGCTTAATGCAA
  TAAAAACACTGTGATCTATTTTGTTTGGAAGTTGACTGACAGCAAGCAATTATTTCCTGC
  GTGAGACTTTTGAGATTCATCAAGTCAATGGGAAAATTCTAAAACAATTTTCTTCTGGCA
  AACCGATTCTAAACATGGCATTGCATTGTCTTCATAACCATTCTCATATAGTAAGCATAG
  TAATACTATTTCCACTTTATATATGGAGAAACGTANGCTCAGAAGTTAAACAATTTACCA
  CATATCATCAGTACGACAGCCAGAATTAAATTAAGTCTGTAGACAATTAGCCTATACTCT
  TAGTACTTGACAGACAGAATATTCAAAACCCATGAGCCTATCACACCCTTCCTTNCATTC
  TTTCATTCCTCATTATTGACCTTTNCCAGTATGGAATAAATTAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000390
• Insert Size: 3590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000390.1, NP_000381.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1962 bp
• Locus ID: 1121
• Cytogenetics: Xq21.2
• Domains: GDI
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.