MARK2 (NM_017490) Human Tagged ORF Clone

CAT#: RC213764

MARK2 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413771) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213764 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MARK2
• Synonyms: EMK-1; EMK1; PAR-1; Par-1b; Par1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213764 representing NM_017490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTACCGGCTCCTCA
  AGACCATTGGCAAGGGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATCCTGACTGGGAAAGAGGTAGC
  TGTGAAGATCATTGACAAGACTCAACTGAACTCCTCCAGCCTCCAGAAACTATTCCGCGAAGTAAGAATA
  ATGAAGGTTTTGAATCATCCCAACATAGTTAAATTATTTGAAGTGATTGAGACTGAGAAAACGCTCTACC
  TTGTCATGGAGTACGCTAGTGGCGGAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGATGAAAGAAAA
  AGAGGCTCGAGCCAAATTCCGCCAGATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTTATTGTCCAT
  AGAGACTTAAAGGCAGAAAACCTGCTCTTGGATGCTGATATGAACATCAAGATTGCAGACTTTGGCTTCA
  GCAATGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCCCCTTATGCTGCCCCAGAACT
  CTTCCAGGGCAAAAAATATGATGGACCCGAGGTGGATGTGTGGAGCCTAGGAGTTATCCTCTATACACTG
  GTCAGCGGATCCCTGCCTTTTGATGGACAGAACCTCAAGGAGCTGCGGGAACGGGTACTGAGGGGAAAAT
  ACCGTATTCCATTCTACATGTCCACGGACTGTGAAAACCTGCTTAAGAAATTTCTCATTCTTAATCCCAG
  CAAGAGAGGCACTTTAGAGCAAATCATGAAAGATCGATGGATGAATGTGGGTCACGAAGATGATGAACTA
  AAGCCTTACGTGGAGCCACTCCCTGACTACAAGGACCCCCGGCGGACAGAGCTGATGGTGTCCATGGGTT
  ATACACGGGAAGAGATCCAGGACTCGCTGGTGGGCCAGAGATACAACGAGGTGATGGCCACCTATCTGCT
  CCTGGGCTACAAGAGCTCCGAGCTGGAAGGCGACACCATCACCCTGAAACCCCGGCCTTCAGCTGATCTG
  ACCAATAGCAGCGCCCCATCCCCATCCCACAAGGTACAGCGCAGCGTGTCGGCCAATCCCAAGCAGCGGC
  GCTTCAGCGACCAGGCTGGTCCTGCCATTCCCACCTCTAATTCTTACTCTAAGAAGACTCAGAGTAACAA
  CGCAGAAAATAAGCGGCCTGAGGAGGACCGGGAGTCAGGGCGGAAAGCCAGCAGCACAGCCAAGGTGCCT
  GCCAGCCCCCTGCCCGGTCTGGAGAGGAAGAAGACCACCCCAACCCCCTCCACGAACAGCGTCCTCTCCA
  CCAGCACAAATCGAAGCAGGAATTCCCCACTTTTGGAGCGGGCCAGCCTCGGCCAGGCCTCCATCCAGAA
  TGGCAAAGACAGCCTAACCATGCCAGGGTCCCGGGCCTCCACGGCTTCTGCTTCTGCCGCAGTCTCTGCG
  GCCCGGCCCCGCCAGCACCAGAAATCCATGTCGGCCTCCGTGCACCCCAACAAGGCCTCTGGGCTGCCCC
  CCACGGAGAGTAACTGTGAGGTGCCGCGGCCCAGCACAGCCCCCCAGCGTGTCCCTGTTGCCTCCCCATC
  CGCCCACAACATCAGCAGCAGTGGTGGAGCCCCAGACCGAACTAACTTCCCCCGGGGTGTGTCCAGCCGA
  AGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAATTTGCCCTACGGTGTGACCCCAG
  CCTCTCCCTCTGGCCACAGCCAGGGCCGGCGGGGGGCCTCTGGGAGCATCTTCAGCAAGTTCACCTCCAA
  GTTTGTACGCAGGAACCTGAATGAACCTGAAAGCAAAGACCGAGTGGAGACGCTCAGACCTCACGTGGTG
  GGCAGTGGCGGCAACGACAAAGAAAAGGAAGAATTTCGGGAGGCCAAGCCCCGCTCCCTCCGCTTCACGT
  GGAGTATGAAGACCACGAGCTCCATGGAGCCCAACGAGATGATGCGGGAGATCCGCAAGGTGCTGGACGC
  GAACAGCTGCCAGAGCGAGCTGCATGAGAAGTACATGCTGCTGTGCATGCACGGCACGCCGGGCCACGAG
  GACTTCGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCGCGGCTCTCTCTCAACGGGGTTCGATTTAAGC
  GGATATCGGGCACCTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCCAACGAGCTGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213764 representing NM_017490
  Red=Cloning site Green=Tags(s)
  
  MIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRI
  MKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVH
  RDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTL
  VSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDEL
  KPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADL
  TNSSAPSPSHKVQRSVSANPKQRRFSDQAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVP
  ASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSA
  ARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSR
  STFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLNEPESKDRVETLRPHVV
  GSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHE
  DFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017490
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017490.1, NM_017490.2, NM_017490.3, NP_059672.1
• RefSeq Size: 2946 bp
• RefSeq ORF: 2238 bp
• Locus ID: 2011
• Cytogenetics: 11q13.1
• Domains: UBA, pkinase, KA1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 82.9 kDa
• Gene Summary: 'This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]'