MARK2 (NM_017490) Human Untagged Clone
CAT#: SC323417
MARK2 (untagged)-Kinase deficient mutant (K49M) of Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1
"NM_017490" in other vectors (7)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | MARK2 |
Synonyms | EMK-1; EMK1; PAR-1; Par-1b; Par1b |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323417 sequence for NM_017490 edited (data generated by NextGen Sequencing)
ATGATTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTAC CGGCTCCTCAAGACCATTGGCAAGGGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATC CTGACTGGGAAAGAGGTAGCTGTGATGATCATTGACAAGACTCAACTGAACTCCTCCAGC CTCCAGAAACTATTCCGCGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTT AAATTATTTGAAGTGATTGAGACTGAGAAAACGCTCTACCTTGTCATGGAGTACGCTAGT GGCGGAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGATGAAAGAAAAAGAGGCTCGA GCCAAATTCCGCCAGATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTTATTGTCCAT AGAGACTTAAAGGCAGAAAACCTGCTCTTGGATGCTGATATGAACATCAAGATTGCAGAC TTTGGCTTCAGCAATGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCC CCTTATGCTGCCCCAGAACTCTTCCAGGGCAAAAAATATGATGGACCCGAGGTGGATGTG TGGAGCCTAGGAGTTATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAG AACCTCAAGGAGCTGCGGGAACGGGTACTGAGGGGAAAATACCGTATTCCATTCTACATG TCCACGGACTGTGAAAACCTGCTTAAGAAATTTCTCATTCTTAATCCCAGCAAGAGAGGC ACTTTAGAGCAAATCATGAAAGATCGATGGATGAATGTGGGTCACGAAGATGATGAACTA AAGCCTTACGTGGAGCCACTCCCTGACTACAAGGACCCCCGGCGGACAGAGCTGATGGTG TCCATGGGTTATACACGGGAAGAGATCCAGGACTCGCTGGTGGGCCAGAGATACAACGAG GTGATGGCCACCTATCTGCTCCTGGGCTACAAGAGCTCCGAGCTGGAAGGCGACACCATC ACCCTGAAACCCCGGCCTTCAGCTGATCTGACCAATAGCAGCGCCCCATCCCCATCCCAC AAGGTACAGCGCAGCGTGTCGGCCAATCCCAAGCAGCGGCGCTTCAGCGACCAGGCTGGT CCTGCCATTCCCACCTCTAATTCTTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAAT AAGCGGCCTGAGGAGGACCGGGAGTCAGGGCGGAAAGCCAGCAGCACAGCCAAGGTGCCT GCCAGCCCCCTGCCCGGTCTGGAGAGGAAGAAGACCACCCCAACCCCCTCCACGAACAGC GTCCTCTCCACCAGCACAAATCGAAGCAGGAATTCCCCACTTTTGGAGCGGGCCAGCCTC GGCCAGGCCTCCATCCAGAATGGCAAAGACAGCCTAACCATGCCAGGGTCCCGGGCCTCC ACGGCTTCTGCTTCTGCCGCAGTCTCTGCGGCCCGGCCCCGCCAGCACCAGAAATCCATG TCGGCCTCCGTGCACCCCAACAAGGCCTCTGGGCTGCCCCCCACGGAGAGTAACTGTGAG GTGCCGCGGCCCAGCACAGCCCCCCAGCGTGTCCCTGTTGCCTCCCCATCCGCCCACAAC ATCAGCAGCAGTGGTGGAGCCCCAGACCGAACTAACTTCCCCCGGGGTGTGTCCAGCCGA AGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAGCAGAATTTGCCCTACGGT GTGACCCCAGCCTCTCCCTCTGGCCACAGCCAGGGCCGGCGGGGGGCCTCTGGGAGCATC TTCAGCAAGTTCACCTCCAAGTTTGTACGCAGRAAYCTGAATGAACCTGAAAGCAAAGAC CGAGTGGAGACGCTCAGACCTCACGTGGTGGGCAGTGGCGGCAACGACAAAGAAAAGGAA GAATTTCGGGAGGCCAAGCCCCGCTCCCTCCGCTTCACGTGGAGTATGAAGACTACGAGC TCCATGGAGCCCAACGAGATGATGCGGGAGATCCGCAAGGTGCTGGACGCGAACAGCTGC CAGAGCGAGCTGCATGAGAAGTACATGCTGCTGTGCATGCACGGCACGCCGGGCCACGAG GACTTCGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCGCGGCTCTCTCTCAACGGGGTT CGATTTAAGCGGATATCGGGCACCTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCC AACGAGCTGAAGCTTTAA Clone variation with respect to NM_017490.3 146 a=>t;1833 g=>r;1836 c=>y;1974 c=>t >OriGene 5' read for mutant NM_017490 unedited
ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAACGAGAGGG ACACGGAGCAGCCCACCTTGGGACACCTTGACTCCAAGCCCAGCAGTAAGTCCAACATGATTCGGGGCCG CAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTACCGGCTCCTCAAGACCATTGGCAAG GGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATCCTGACTGGGAAAGAGGTAGCTGTGATGATCATTG ACAAGACTCAACTGAACTCCTCCAGCCTCCAGAAACTATTTCCGCGAAGTAAGAAATAATGAAGGTTTTG ATTCATCCCCACCATAGTTAATTATTTGAGTGATTGAAACTGAGAAAACGCTCTACTTGTCATGGAGTAC GCTAGTGCGGGAGAGTATTTGAATACTAGGTGCCAATGGCAGGTGAAGAAAAAAAGGCTGAGCAAATCCG GCCGGATGGTGTCGGTTGGAATTCTGTCCCAGAGTTAATGGCATTAGAGCTTAAGGAGAAAACTGCTTTG GTGCCTGATGAACTCAGATGCGAACTTGGTTAGAATGGATTCCCTTGGAAAGCGGAACCTTCTGGATCCC CTAAGCGCGCCGAACCTTCAGGCAAATATGGACCAGTGAGTGTGGACTAGTATCCTTACCTGTACGGATC TCGCTTGAGACGACTAAGCTCGACGACTAAGGAATCGATCTTCATGTCGCGTGACCGTGAGTCTCTAACC GGGA >SC323417 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCATGGCTCACCTCCGG GGATTTGCCAACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTCACCAAGCTCGACCGCATTGGCAAGG GCTCGTTTGGGGAGGTCTACAAGGGCATCGATAACCACACAAAGG |
Restriction Sites | Please inquire |
ACCN | NM_017490 |
Insert Size | 2700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017490.1, NP_059672.1 |
RefSeq Size | 2946 bp |
RefSeq ORF | 2238 bp |
Locus ID | 2011 |
Cytogenetics | 11q13.1 |
Domains | UBA, pkinase, KA1 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | 'This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]' Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, lacks an in-frame exon, and uses an alternate in-frame splice site, compared to variant 4. The resulting isoform (a) has a shorter N-terminus and lacks an internal segment and 1 internal residue, compared to isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114124 | MARK2 (untagged)-Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1 |
USD 1,500.00 |
|
RC213764 | MARK2 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1 |
USD 820.00 |
|
RG213764 | MARK2 (GFP-tagged) - Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1 |
USD 900.00 |
|
RC213764L1 | Lenti ORF clone of Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1, Myc-DDK-tagged |
USD 1,260.00 |
|
RC213764L2 | Lenti ORF clone of Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1, mGFP tagged |
USD 1,020.00 |
|
RC213764L3 | Lenti ORF clone of Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1, Myc-DDK-tagged |
USD 1,020.00 |
|
RC213764L4 | Lenti ORF clone of Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1, mGFP tagged |
USD 1,020.00 |
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