MARK2 (NM_017490) Human Untagged Clone

CAT#: SC114124

MARK2 (untagged)-Human MAP/microtubule affinity-regulating kinase 2 (MARK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK2
• Synonyms: EMK-1; EMK1; PAR-1; Par-1b; Par1b
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017490 edited
  ATGATTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTAC
  CGGCTCCTCAAGACCATTGGCAAGGGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATC
  CTGACTGGGAAAGAGGTAGCTGTGAAGATCATTGACAAGACTCAACTGAACTCCTCCAGC
  CTCCAGAAACTATTCCGCGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTT
  AAATTATTTGAAGTGATTGAGACTGAGAAAACGCTCTACCTTGTCATGGAGTACGCTAGT
  GGCGGAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGATGAAAGAAAAAGAGGCTCGA
  GCCAAATTCCGCCAGATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTTATTGTCCAT
  AGAGACTTAAAGGCAGAAAACCTGCTCTTGGATGCTGATATGAACATCAAGATTGCAGAC
  TTTGGCTTCAGCAATGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCC
  CCTTATGCTGCCCCAGAACTCTTCCAGGGCAAAAAATATGATGGACCCGAGGTGGATGTG
  TGGAGCCTAGGAGTTATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAG
  AACCTCAAGGAGCTGCGGGAACGGGTACTGAGGGGAAAATACCGTATTCCATTCTACATG
  TCCACGGACTGTGAAAACCTGCTTAAGAAATTTCTCATTCTTAATCCCAGCAAGAGAGGC
  ACTTTAGAGCAAATCATGAAAGATCGATGGATGAATGTGGGTCACGAAGATGATGAACTA
  AAGCCTTACGTGGAGCCACTCCCTGACTACAAGGACCCCCGGCGGACAGAGCTGATGGTG
  TCCATGGGTTATACACGGGAAGAGATCCAGGACTCGCTGGTGGGCCAGAGATACAACGAG
  GTGATGGCCACCTATCTGCTCCTGGGCTACAAGAGCTCCGAGCTGGAAGGCGACACCATC
  ACCCTGAAACCCCGGCCTTCAGCTGATCTGACCAATAGCAGCGCCCCATCCCCATCCCAC
  AAGGTACAGCGCAGCGTGTCGGCCAATCCCAAGCAGCGGCGCTTCAGCGACCAGGCTGGT
  CCTGCCATTCCCACCTCTAATTCTTACTCTAAGAAGACTCAGAGTAACAACGCAGAAAAT
  AAGCGGCCTGAGGAGGACCGGGAGTCAGGGCGGAAAGCCAGCAGCACAGCCAAGGTGCCT
  GCCAGCCCCCTGCCCGGTCTGGAGAGGAAGAAGACCACCCCAACCCCCTCCACGAACAGC
  GTCCTCTCCACCAGCACAAATCGAAGCAGGAATTCCCCACTTTTGGAGCGGGCCAGCCTC
  GGCCAGGCCTCCATCCAGAATGGCAAAGACAGCACAGCCCCCCAGCGTGTCCCTGTTGCC
  TCCCCATCCGCCCACAACATCAGCAGCAGTGGTGGAGCCCCAGACCGAACTAACTTCCCC
  CGGGGTGTGTCCAGCCGAAGCACCTTCCATGCTGGGCAGCTCCGACAGGTGCGGGACCAG
  CAGAATTTGCCCTACGGTGTGACCCCAGCCTCTCCCTCTGGCCACAGCCAGGGCCGGCGG
  GGGGCCTCTGGGAGCATCTTCAGCAAGTTCACCTCCAAGTTTGTACGCAGAAATCTGTCT
  TTCAGGTTTGCCAGAAGACCTCACGTGGTGGGCAGTGGCGGCAACGACAAAGAAAAGGAA
  GAATTTCGGGAGGCCAAGCCCCGCTCCCTCCGCTTCACGTGGAGTATGAAGACTACGAGC
  TCCATGGAGCCCAACGAGATGATGCGGGAGATCCGCAAGGTGCTGGACGCGAACAGCTGC
  CAGAGCGAGCTGCATGAGAAGTACATGCTGCTGTGCATGCACGGCACGCCGGGCCACGAG
  GACTTCGTGCAGTGGGAGATGGAGGTGTGCAAACTGCCGCGGCTCTCTCTCAACGGGGTT
  CGATTTAAGCGGATATCGGGCACCTCCATGGCCTTCAAAAACATTGCCTCCAAAATAGCC
  AACGAGCTGAAGCTTTAACAGGCTGCCAGGAGCGGGGGCGGCGGGGGCGGGCCAGCTGGA
  CGGGCTGCCGGCCGCTGCGCCGCCCCACCTGGGCGAGACTGCAGCGATGGATTGGTGTGT
  CTCCCCTGCTGGCACTTCTCCCCTCCCTGGCCCTTCTCAGTTTTCTCTTACATGTTTGTG
  GGGGGTGGGAGATTGTTCTCCAGCCCCCCACATTCACCCCTGCCCAGAGATTCCCCCTTC
  TCCTCTCCCCTACTGGAGGCAAAGGAAGGGGAGGGTGGATGGGGGGGCAGGGCTCCCCCT
  CGGTACTGCGGTTGCACAGAGTATTTCGCCTAAACCAAGAAATTTTTTATTACCAAAAAG
  AAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAA
  CAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTG
  CCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACT
  AGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTG
  GGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCAC
  AATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTC
  CCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGT
  AGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCT
  ACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTG
  TCCTTCTGATTTTAAAATAACTATACCAGCGGGAGGACGTCCAGACACAGCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017490 unedited
  GTAATACGACTTTCTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAACGAGAGGGACAC
  GGAGCAGCCCACCTTGGGACACCTTGACTCCAAGCCCAGCAGTAAGTCCAACATGATTCG
  GGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCCCCACATTGGAAACTACCGGCTCCT
  CAAGACCATTGGCAAGGGTAATTTTGCCAAGGTGAAGTTGGCCCGACACATCCTGACTGG
  GAAAGAGGTAGCTGTGAAGATCATTGACAAGACTCAACTGAACTCCTCCAGCCTCCAGAA
  ACTATTCCGCGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAATTATT
  TGAAGTGATTGAGACTGAGAAAACGCTCTACCTTGTCATGGAGTACGCTAGTGGCGGAGA
  GGTATTTGATTACCTAGTGGCTCATGGCAGGATGAAAGAAAAAGAGGCTCGAGCCAAATT
  CCGCCAGATAGTGTCTGCTGTGCAGTACTGTCACCAGAAGTTTATTGTCCATAGAGACTT
  AAAGGCAGAAAACCTGCTCTTGGATGCTGATATGAACATCAAGATTGCAGACTTTGGCTT
  CAGCAATGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCCCCTTATGC
  TGCCCCAGAACTCTTCCAGGGCANAAAATATGATGGACCCGAGGTGGATGTGTGGAGCCT
  ANGAGTTATCCTCTATACACTGGTCAGCGGATCCCTGCCTTTTGATGGACAGAACCCTCA
  AGAGCTGCGGGAACGGGTACTGANGGGGAAAATACCGTATTCCATTCTACATGTCCACGG
  ACTGTGGAAACCTGCTTAAGAAATTCTCATTNCTAATCCCAGCAGAGAGCACTTANAGCN
  ATCATGAAGATCGATNATGAATGGGGTNACGAANATGAGAACTAAGNCCTACGTGGACCA
  CTNCTGACTCAGGACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017490 unedited
  GCCTATGGACCGCGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTTTGGTA
  ATAAAAAATTTCTTGGTTTAGGCGAAATACTCTGTGCAACCGCAGTACCGAGGGGGAGCC
  CTGCCCCCCCATCCACCCTCCCCTTCCTTTGCCTCCAGTAGGGGAGAGGAGAAGGGGGAA
  TCTCTGGGCAGGGGTGAATGTGGGGGNCTNNNNAANNNNNCCCCNCCCCCCNCCCNNCCC
  CCCCCCNCCNNCNCCCCCTTTCCCCCCCCCNCCNCCCCCCCCCCCCCCCCCNNCCCCCCC
  CCCCCNCCCCCNCCCCCCCCNNNCNCCCCCCCCCCCCCCCCCCCNCCCCCCCNNNCCCCC
  NCCCCCCCCNCCCCCCCCCCNCNCCCCCCCTCCCCCCCNNCCCCCCCCCNCCCCCCCCCN
  CCCCCCCCNNCCCCCCCCCCCCCCCCCCNCCCCCNCNNCCCCCCCCCCCNCCCCNCCCCC
  CCNNCCCCCCCCNCCCCCCCCCCNNNCCCCCCCNCCCCCCCCCCCCCCCCCNNCCCCCCN
  CNCCCNCCCCCCCCCCCCCCCCCCNCNNNCCCCCCCCCCNNCCNCCCCNCCCNNCCCCNN
  NNCCCCCNNCCCNNCCCCNNCCCCCNCCCCCCCCNCCCCTCNNCCCCCNCCCCCCNCCCC
  CNCCCCCTCCCCCCCCNTCCCCCCCCTCCCCCTCTTCTTCTCCCTCNCTGTCTTCCTTCC
  CTCCCCCTTCTTTTCCCCCCNCTTTCCCCTTTTCCTCCCCCCCTCCTCTTTTNTCCCCCC
  TTTTCTTGTTTTTCCCTTCCCCCCTTTCTCCTCTCTTTTTCCCTTTTTTTCCCTCTCCCT
  TTTCCCCCTCCCTTTCTCCCTTCTCCTTTCTCTCCGTCCTTCTTTCTTCTTTTTTCTTTC
  TCTTTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017490
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017490.1, NP_059672.1
• RefSeq Size: 2946 bp
• RefSeq ORF: 2238 bp
• Locus ID: 2011
• Cytogenetics: 11q13.1
• Domains: UBA, pkinase, KA1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, lacks an in-frame exon, and uses an alternate in-frame splice site, compared to variant 4. The resulting isoform (a) has a shorter N-terminus and lacks an internal segment and 1 internal residue, compared to isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.