SPIRE1 (NM_020148) Human Tagged ORF Clone

CAT#: RC213848

SPIRE1 (Myc-DDK-tagged)-Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY412552) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213848 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SPIRE1 protein (Cat# TP313848). The protein was produced from HEK293T cells transfected with SPIRE1 cDNA clone (Cat# RC213848) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPIRE1
• Synonyms: Spir-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213848 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACACGGTGGAAGCTGACGGTAGCAATGATGAGGGCTATGAGGCTGCAGAAGAAGGCCTGGGAG
  ATGAAGATGAAAAGAGAAAAATCTCAGCTATTCGGTCATATAGAGATGTCATGAAGTTGTGTGCTGCTCA
  TCTCCCTACTGAATCAGATGCACCAAATCATTATCAGGCAGTATGTCGTGCACTGTTTGCAGAAACAATG
  GAGCTCCATACATTTCTGACCAAAATTAAGAGTGCGAAAGAGAATCTTAAGAAGATTCAAGAAATGGAAA
  AGAGCGATGAATCTAGCACAGACTTGGAAGAGCTGAAAAACGCTGACTGGGCACGATTCTGGGTACAGGT
  GATGAGGGATTTGAGGAATGGGGTAAAACTTAAGAAGGTCCAAGAGCGGCAGTACAACCCTTTGCCCATT
  GAATATCAGCTCACCCCTTATGAGATGTTAATGGATGACATTCGCTGCAAAAGATACACCTTGCGAAAAG
  TGATGGTGAATGGTGATATTCCCCCTCGGTTAAAAAAGAGTGCTCATGAAATCATCCTCGACTTCATCAG
  ATCCAGACCTCCTTTAAATCCAGTCTCAGCCAGAAAACTGAAACCAACTCCACCACGGCCACGGAGCCTC
  CATGAAAGAATATTAGAAGAAATTAAAGCAGAAAGAAAGCTGCGGCCTGTATCACCAGAGGAGATTAGAC
  GTAGCAGATTAGATGTGACTACCCCTGAATCTACAAAGAATCTTGTGGAGTCATCTATGGTGAATGGAGG
  TTTGACATCACAAACAAAAGAAAACGGGTTAAGTACCTCACAGCAGGTGCCTGCACAGCGGAAGAAGCTC
  CTCAGAGCCCCAACTCTGGCCGAACTGGACAGCTCTGAGTCTGAGGAAGAAACGCTGCACAAGTCGACCA
  GCAGCAGCAGCGTGTCTCCCTCTTTCCCTGAAGAGCCAGTCCTGGAGGCCGTGTCCACAAGGAAGAAGCC
  TCCAAAATTCCTGCCCATATCATCAACACCCCAGCCAGAGAGACGGCAGCCACCCCAGAGACGACATTCC
  ATTGAAAAGGAAACGCCTACTAACGTGAGGCAGTTCCTGCCGCCTTCCAGGCAGAGTTCCCGCTCTCTTG
  AGGAATTCTGCTACCCAGTGGAATGCCTCGCTCTTACTGTGGAAGAAGTGATGCATATTCGCCAGGTCCT
  GGTGAAGGCAGAGCTGGAAAAATACCAACAGTATAAAGACATCTACACCGCCTTGAAAAAAGGAAAGCTC
  TGCTTTTGTTGCCGAACCAGGAGGTTTTCCTTCTTCACTTGGTCTTATACCTGTCAGTTCTGTAAGAGGC
  CGGTGTGCTCACAGTGTTGCAAAAAGATGCGGCTGCCCTCCAAACCATACTCCACTCTTCCTATCTTTTC
  ATTGGGACCTTCTGCTCTGCAAAGAGGGGAAAGTAGTATGAGGTCAGAAAAACCCTCCACTGCCCATCAT
  CGGCCACTTCGGAGCATTGCCAGGTTCTCCTCAAAATCTAAGTCTATGGACAAATCAGATGAAGAACTCC
  AGTTTCCCAAAGAGTTGATGGAGGACTGGAGCACCATGGAGGTGTGTGTGGACTGCAAGAAGTTCATTTC
  GGAAATCATCAGTTCAAGCCGGCGCAGTCTGGTGTTGGCCAACAAAAGGGCCCGATTGAAAAGGAAAACG
  CAGTCTTTCTACATGTCCTCGCCAGGCCCCTCGGAGTACTGCCCTTCAGAGAGGACGATCAGTGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213848 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANTVEADGSNDEGYEAAEEGLGDEDEKRKISAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETM
  ELHTFLTKIKSAKENLKKIQEMEKSDESSTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPI
  EYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSL
  HERILEEIKAERKLRPVSPEEIRRSRLDVTTPESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKL
  LRAPTLAELDSSESEEETLHKSTSSSSVSPSFPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHS
  IEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDIYTALKKGKL
  CFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHH
  RPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKT
  QSFYMSSPGPSEYCPSERTISEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020148
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020148.1, NP_064533.1
• RefSeq Size: 5402 bp
• RefSeq ORF: 2229 bp
• Locus ID: 56907
• Protein Pathways: Dorso-ventral axis formation
• MW: 67.1 kDa
• Gene Summary: Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]). [supplied by OMIM, Mar 2008]