OR2C1 (NM_012368) Human Tagged ORF Clone

CAT#: RC213938

OR2C1 (Myc-DDK-tagged)-Human olfactory receptor, family 2, subfamily C, member 1 (OR2C1)

Product Images

Western blot validation of overexpression lysate (Cat# LY415814) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213938 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR2C1
• Synonyms: OLFmf3; OR2C2P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213938 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGGGTGAATGATAGCTCCTTGCAGGGCTTTGTTCTGATGGGCATATCGGACCATCCCCAGCTGG
  AGATGATCTTTTTTATAGCCATCCTCTTCTCCTATTTGCTGACCCTACTTGGGAACTCAACCATCATCTT
  GCTTTCCCGCCTGGAGGCCCGGCTCCATACACCCATGTACTTCTTCCTCAGCAACCTCTCCTCCTTGGAC
  CTTGCTTTCGCTACTAGTTCAGTCCCCCAAATGCTGATCAATTTATGGGGACCAGGCAAGACCATCAGCT
  ATGGTGGCTGCATAACCCAGCTCTATGTCTTCCTTTGGCTGGGGGCCACCGAGTGCATCCTGCTGGTGGT
  GATGGCATTTGACCGCTACGTGGCAGTGTGCCGGCCCCTCCGCTACACCGCCATCATGAACCCCCAGCTC
  TGCTGGCTGCTGGCTGTGATTGCCTGGCTGGGTGGCTTGGGCAACTCTGTGATCCAGTCAACATTCACTC
  TGCAGCTCCCATTGTGTGGGCACCGGAGGGTGGAGGGATTCCTCTGCGAGGTGCCTGCCATGATCAAACT
  GGCCTGTGGCGACACAAGTCTCAACCAGGCTGTGCTCAATGGTGTCTGCACCTTCTTCACTGCAGTCCCA
  CTAAGCATCATCGTGATCTCCTACTGCCTCATTGCTCAGGCAGTGCTGAAAATCCGCTCTGCAGAGGGGA
  GGCGAAAGGCGTTCAATACGTGCCTCTCCCATCTGCTGGTGGTGTTCCTCTTCTATGGCTCAGCCAGCTA
  TGGGTATCTGCTTCCGGCCAAGAACAGCAAACAGGACCAGGGCAAGTTCATTTCCCTGTTCTACTCGTTG
  GTCACACCCATGGTGAATCCCCTCATCTACACGCTGCGGAACATGGAAGTGAAGGGCGCACTGAGGAGGT
  TGCTGGGGAAAGGAAGAGAAGTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213938 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDGVNDSSLQGFVLMGISDHPQLEMIFFIAILFSYLLTLLGNSTIILLSRLEARLHTPMYFFLSNLSSLD
  LAFATSSVPQMLINLWGPGKTISYGGCITQLYVFLWLGATECILLVVMAFDRYVAVCRPLRYTAIMNPQL
  CWLLAVIAWLGGLGNSVIQSTFTLQLPLCGHRRVEGFLCEVPAMIKLACGDTSLNQAVLNGVCTFFTAVP
  LSIIVISYCLIAQAVLKIRSAEGRRKAFNTCLSHLLVVFLFYGSASYGYLLPAKNSKQDQGKFISLFYSL
  VTPMVNPLIYTLRNMEVKGALRRLLGKGREVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012368
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012368.1, NM_012368.2, NP_036500.1
• RefSeq Size: 1036 bp
• RefSeq ORF: 939 bp
• Locus ID: 4993
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 34.5 kDa
• Gene Summary: 'Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]'