BACH2 (NM_021813) Human Tagged ORF Clone

CAT#: RC214061

BACH2 (Myc-DDK-tagged)-Human BTB and CNC homology 1, basic leucine zipper transcription factor 2 (BACH2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402878) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214061 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BACH2 protein (Cat# TP314061). The protein was produced from HEK293T cells transfected with BACH2 cDNA clone (Cat# RC214061) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BACH2
• Synonyms: BTBD25; IMD60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214061 representing NM_021813.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTGTGGATGAGAAGCCTGACTCCCCCATGTATGTGTATGAGTCCACAGTCCACTGCACCAACATC
  CTCCTGGGCCTCAATGACCAGCGGAAAAAGGATATTCTCTGTGACGTGACTTTGATCGTGGAGAGGAAG
  GAGTTCCGGGCCCACCGGGCTGTGCTGGCCGCATGCAGTGAATATTTTTGGCAGGCGCTGGTTGGACAG
  ACAAAAAATGATTTGGTGGTCAGCTTGCCTGAGGAGGTCACAGCCAGGGGCTTTGGGCCGCTGTTACAG
  TTTGCCTACACTGCCAAGCTGTTACTCAGCAGAGAAAACATCCGCGAGGTCATCCGCTGTGCTGAGTTC
  CTGCGCATGCACAACCTGGAGGACTCCTGCTTCAGCTTCCTGCAGACCCAGCTCCTGAACAGTGAGGAT
  GGCCTGTTTGTGTGCCGGAAGGATGCTGCGTGCCAGCGCCCACACGAGGACTGCGAGAACTCTGCAGGA
  GAGGAGGAGGATGAAGAGGAGGAGACGATGGATTCAGAGACGGCCAAGATGGCTTGCCCCAGGGACCAG
  ATGCTTCCAGAGCCCATCAGCTTTGAGGCCGCCGCCATCCCCGTAGCAGAGAAGGAAGAAGCCCTGCTG
  CCCGAGCCTGACGTGCCCACAGACACCAAGGAGAGCTCAGAAAAGGACGCGTTAACGCAGTACCCCAGA
  TACAAGAAATACCAGCTTGCATGTACCAAGAATGTCTATAATGCATCATCACACAGTACCTCAGGTTTT
  GCAAGCACATTCCGGGAAGATAACTCTAGCAACAGCCTCAAGCCGGGGCTTGCCAGGGGGCAGATTAAA
  AGTGAGCCGCCCAGTGAAGAGAATGAGGAAGAGAGCATCACGCTCTGCCTGTCTGGAGATGAGCCTGAC
  GCCAAGGACAGAGCGGGGGATGTCGAGATGGACCGGAAACAGCCCAGCCCTGCCCCTACCCCCACGGCC
  CCAGCTGGGGCCGCCTGCCTGGAGAGATCCAGGAGCGTGGCCTCGCCCTCCTGCTTAAGGTCTCTGTTC
  AGCATAACGAAAAGTGTGGAGCTGTCTGGCCTGCCCAGTACATCTCAGCAGCACTTTGCCAGGAGTCCA
  GCCTGCCCTTTTGACAAGGGGATCACTCAGGGTGACCTTAAAACTGACTACACCCCTTTCACAGGGAAT
  TATGGACAGCCCCACGTGGGCCAGAAGGAGGTGTCCAACTTCACCATGGGGTCGCCCCTCAGGGGGCCT
  GGGTTGGAGGCTCTCTGTAAACAGGAGGGAGAGCTGGACCGGAGGAGCGTGATCTTCTCCTCCAGCGCT
  TGTGACCAAGTGAGCACCTCGGTGCATTCTTATTCTGGGGTGAGCAGTTTGGACAAAGACCTCTCTGAG
  CCGGTGCCAAAGGGTCTGTGGGTGGGAGCCGGCCAGTCCCTCCCCAGCTCGCAGGCCTACTCCCACGGT
  GGGCTGATGGCCGACCACTTGCCAGGAAGGATGCGGCCCAACACCAGCTGCCCGGTGCCAATCAAAGTC
  TGCCCTCGCTCACCCCCCTTGGAGACCAGGACCAGGACTTCCAGCTCCTGCTCTTCCTATTCCTACGCG
  GAGGACGGGAGCGGGGGCTCACCCTGCAGCCTCCCTCTCTGTGAGTTCTCCTCCTCGCCCTGTTCCCAG
  GGAGCCAGATTCCTTGCCACAGAACATCAGGAACCAGGCCTGATGGGAGATGGAATGTACAACCAAGTG
  CGGCCCCAAATTAAATGTGAGCAGTCTTATGGAACCAACTCCAGTGACGAATCCGGATCGTTCTCGGAA
  GCAGACAGTGAGTCGTGTCCTGTGCAGGACAGGGGCCAGGAGGTAAAACTTCCTTTTCCTGTAGATCAA
  ATCACAGATCTTCCAAGGAACGATTTCCAGATGATGATTAAAATGCACAAGCTAACCTCAGAACAGTTA
  GAGTTTATTCATGATGTCCGACGGCGCAGCAAGAACCGCATCGCGGCCCAGCGCTGCCGCAAAAGGAAA
  CTGGACTGTATTCAGAATTTAGAATGTGAAATCCGCAAATTGGTGTGTGAGAAAGAGAAACTGTTGTCA
  GAGAGGAATCAACTGAAAGCATGCATGGGGGAACTGTTGGACAACTTCTCCTGCCTTTCCCAGGAAGTT
  TGCCGAGACATCCAGAGCCCCGAGCAGATCCAGGCCCTGCATCGGTATTGCCCTGTCCTCAGACCCATG
  GACTTGCCCACGGCCTCCAGTATTAACCCTGCGCCCTTGGGTGCTGAGCAGAACATTGCGGCCTCCCAA
  TGCGCAGTGGGGGAAAACGTGCCCTGCTGCTTGGAGCCAGGCGCGGCTCCCCCCGGACCCCCCTGGGCA
  CCCAGCAACACCTCCGAGAATTGTACCTCTGGGAGGAGACTAGAAGGCACTGACCCGGGAACCTTCTCA
  GAGAGAGGACCTCCTCTTGAACCCAGGAGCCAAACAGTGACCGTGGACTTCTGCCAGGAAATGACTGAT
  AAGTGTACAACTGACGAACAGCCCAGGAAAGATTATACC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC214061
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSVDEKPDSPMYVYESTVHCTNILLGLNDQRKKDILCDVTLIVERKEFRAHRAVLAACSEYFWQALVGQ
  TKNDLVVSLPEEVTARGFGPLLQFAYTAKLLLSRENIREVIRCAEFLRMHNLEDSCFSFLQTQLLNSED
  GLFVCRKDAACQRPHEDCENSAGEEEDEEEETMDSETAKMACPRDQMLPEPISFEAAAIPVAEKEEALL
  PEPDVPTDTKESSEKDALTQYPRYKKYQLACTKNVYNASSHSTSGFASTFREDNSSNSLKPGLARGQIK
  SEPPSEENEEESITLCLSGDEPDAKDRAGDVEMDRKQPSPAPTPTAPAGAACLERSRSVASPSCLRSLF
  SITKSVELSGLPSTSQQHFARSPACPFDKGITQGDLKTDYTPFTGNYGQPHVGQKEVSNFTMGSPLRGP
  GLEALCKQEGELDRRSVIFSSSACDQVSTSVHSYSGVSSLDKDLSEPVPKGLWVGAGQSLPSSQAYSHG
  GLMADHLPGRMRPNTSCPVPIKVCPRSPPLETRTRTSSSCSSYSYAEDGSGGSPCSLPLCEFSSSPCSQ
  GARFLATEHQEPGLMGDGMYNQVRPQIKCEQSYGTNSSDESGSFSEADSESCPVQDRGQEVKLPFPVDQ
  ITDLPRNDFQMMIKMHKLTSEQLEFIHDVRRRSKNRIAAQRCRKRKLDCIQNLECEIRKLVCEKEKLLS
  ERNQLKACMGELLDNFSCLSQEVCRDIQSPEQIQALHRYCPVLRPMDLPTASSINPAPLGAEQNIAASQ
  CAVGENVPCCLEPGAAPPGPPWAPSNTSENCTSGRRLEGTDPGTFSERGPPLEPRSQTVTVDFCQEMTD
  KCTTDEQPRKDYT
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC214061 also available, TP314061M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021813
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021813.1, NP_068585.1
• RefSeq Size: 9120 bp
• RefSeq ORF: 2526 bp
• Locus ID: 60468
• UniProt ID: Q9BYV9
• Cytogenetics: 6q15
• Domains: BTB, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• MW: 92.5 kDa
• Gene Summary: Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (PubMed:17018862). Positively regulates the nuclear import of actin (By similarity).[UniProtKB/Swiss-Prot Function]