PIK3AP1 (NM_152309) Human Tagged ORF Clone

CAT#: RC214125

PIK3AP1 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY403461) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214125 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PIK3AP1 protein (Cat# TP314125). The protein was produced from HEK293T cells transfected with PIK3AP1 cDNA clone (Cat# RC214125) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIK3AP1
• Synonyms: BCAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214125 representing NM_152309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCAGGGGTGCCCAGAGGATGCGACATCCTCATCGTCTACAGCCCGGATGCCGAGGAATGGT
  GCCAGTACCTGCAGACCCTGTTCCTGTCCAGTCGGCAGGTCCGCAGCCAGAAGATACTGACTCACAGGCT
  GGGCCCCGAGGCCTCCTTCTCGGCAGAGGACCTAAGCCTTTTCCTCAGCACCCGCTGTGTCGTGGTGCTG
  CTGTCCGCGGAGCTGGTGCAGCACTTCCACAAGCCCTCCTTGCTGCCCCTGCTGCAGAGAGCTTTCCATC
  CTCCGCACCGCGTGGTCAGGCTGCTCTGCGGCGTGCGGGACAGCGAGGAGTTCCTAGACTTCTTTCCAGA
  TTGGGCCCATTGGCAGGAGCTCACCTGTGACGATGAGCCAGAGACCTACGTGGCAGCTGTGAAAAAAGCC
  ATTTCCGAAGATTCTGGCTGTGACTCAGTCACTGACACTGAGCCTGAGGACGAGAAGGTTGTTTCCTACT
  CGAAGCAGCAGAACCTGCCGACGGTGACTTCACCTGGGAACCTGATGGTGGTGCAGCCGGACCGCATTCG
  CTGTGGGGCAGAAACCACTGTCTATGTTATTGTGAGATGTAAGCTGGATGACAGGGTGGCGACAGAAGCA
  GAGTTTTCTCCTGAGGATTCTCCCTCTGTAAGGATGGAAGCCAAGGTGGAGAATGAGTACACCATTTCAG
  TGAAGGCTCCCAACCTTTCATCTGGGAACGTTTCTCTGAAGATATATTCTGGAGACTTAGTGGTGTGTGA
  AACCGTTATCAGCTATTATACTGACATGGAAGAAATTGGGAATTTATTGTCCAATGCCGCGAATCCTGTG
  GAATTCATGTGTCAGGCCTTTAAAATTGTGCCCTACAACACAGAGACCCTTGATAAACTGCTAACCGAAT
  CCCTGAAGAACAATATCCCTGCAAGCGGACTGCACCTCTTTGGAATCAACCAGCTGGAAGAAGAAGATAT
  GATGACAAATCAGAGGGATGAAGAGCTGCCCACCCTGTTGCATTTTGCTGCGAAGTATGGACTGAAGAAC
  CTCACTGCCTTGTTGCTCACCTGCCCAGGAGCCCTGCAGGCGTACAGCGTGGCCAACAAGCATGGCCACT
  ACCCCAACACCATCGCTGAGAAACACGGCTTCAGGGACCTGCGGCAGTTCATCGACGAGTATGTGGAAAC
  GGTGGACATGCTCAAGAGTCACATTAAAGAGGAACTGATGCACGGGGAGGAGGCTGATGCTGTGTACGAG
  TCCATGGCCCACCTTTCCACAGACCTGCTTATGAAATGCTCGCTCAACCCCGGCTGTGACGAGGATCTCT
  ATGAGTCCATGGCTGCCTTTGTCCCAGCTGCCACTGAAGACCTCTATGTTGAAATGCTTCAGGCCAGTAC
  ATCTAACCCAATCCCTGGAGATGGTTTCTCTCGGGCCACTAAGGACTCTATGATCCGCAAGTTTTTAGAA
  GGCAACAGCATGGGAATGACCAATCTGGAGAGAGATCAGTGCCATCTTGGTCAGGAAGAAGATGTTTATC
  ACACGGTGGATGACGATGAGGCCTTTTCTGTGGACTTGGCCAGCAGGCCCCCTGTCCCAGTGCCCAGACC
  AGAGACCACTGCTCCTGGTGCTCACCAGCTGCCTGACAACGAACCATACATTTTTAAAGTTTTTGCAGAA
  AAAAGTCAAGAGCGGCCTGGGAATTTCTACGTTTCCTCAGAGAGCATCAGGAAAGGGCCGCCCGTCAGAC
  CATGGAGGGACAGGCCCCAGTCAAGTATATATGACCCTTTTGCGGGAATGAAAACGCCAGGCCAGCGGCA
  GCTTATCACCCTCCAGGAGCAGGTGAAGCTGGGCATTGTCAACGTGGATGAGGCTGTGCTCCACTTCAAA
  GAGTGGCAGCTCAACCAGAAGAAACGATCGGAGTCCTTTCGTTTCCAGCAGGAAAATCTTAAACGGCTAA
  GAGACAGCATCACCCGAAGACAGAGAGAGAAGCAAAAATCAGGAAAGCAGACAGACTTGGAGATCACGGT
  CCCAATTCGGCACTCACAGCACCTGCCTGCAAAAGTGGAGTTTGGAGTCTATGAGAGTGGCCCCAGGAAA
  AGTGTCATTCCCCCTAGGACGGAGCTGAGACGAGGAGACTGGAAAACAGACAGCACCTCCAGCACAGCAA
  GTAGCACAAGTAACCGCTCCAGCACCCGGAGCCTCCTCAGTGTGAGCAGCGGGATGGAAGGGGACAACGA
  GGATAATGAAGTCCCTGAGGTTACCAGAAGTCGCAGTCCAGGCCCCCCACAAGTGGATGGGACACCCACC
  ATGTCCCTCGAGAGACCCCCCAGGGTGCCTCCGAGAGCTGCCTCACAGAGGCCTCCGACCAGGGAGACCT
  TCCATCCTCCTCCACCTGTTCCACCCAGAGGACGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214125 representing NM_152309
  Red=Cloning site Green=Tags(s)
  
  MAASGVPRGCDILIVYSPDAEEWCQYLQTLFLSSRQVRSQKILTHRLGPEASFSAEDLSLFLSTRCVVVL
  LSAELVQHFHKPSLLPLLQRAFHPPHRVVRLLCGVRDSEEFLDFFPDWAHWQELTCDDEPETYVAAVKKA
  ISEDSGCDSVTDTEPEDEKVVSYSKQQNLPTVTSPGNLMVVQPDRIRCGAETTVYVIVRCKLDDRVATEA
  EFSPEDSPSVRMEAKVENEYTISVKAPNLSSGNVSLKIYSGDLVVCETVISYYTDMEEIGNLLSNAANPV
  EFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEEDMMTNQRDEELPTLLHFAAKYGLKN
  LTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKSHIKEELMHGEEADAVYE
  SMAHLSTDLLMKCSLNPGCDEDLYESMAAFVPAATEDLYVEMLQASTSNPIPGDGFSRATKDSMIRKFLE
  GNSMGMTNLERDQCHLGQEEDVYHTVDDDEAFSVDLASRPPVPVPRPETTAPGAHQLPDNEPYIFKVFAE
  KSQERPGNFYVSSESIRKGPPVRPWRDRPQSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFK
  EWQLNQKKRSESFRFQQENLKRLRDSITRRQREKQKSGKQTDLEITVPIRHSQHLPAKVEFGVYESGPRK
  SVIPPRTELRRGDWKTDSTSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEVPEVTRSRSPGPPQVDGTPT
  MSLERPPRVPPRAASQRPPTRETFHPPPPVPPRGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152309
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152309.2, NP_689522.2
• RefSeq Size: 4817 bp
• RefSeq ORF: 2418 bp
• Locus ID: 118788
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway
• MW: 90.2 kDa
• Gene Summary: Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. [UniProtKB/Swiss-Prot Function]