PIK3AP1 (NM_152309) Human Tagged ORF Clone

CAT#: RG214125

PIK3AP1 (GFP-tagged) - Human phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIK3AP1
• Synonyms: BCAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214125 representing NM_152309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCAGGGGTGCCCAGAGGATGCGACATCCTCATCGTCTACAGCCCGGATGCCGAGGAATGGT
  GCCAGTACCTGCAGACCCTGTTCCTGTCCAGTCGGCAGGTCCGCAGCCAGAAGATACTGACTCACAGGCT
  GGGCCCCGAGGCCTCCTTCTCGGCAGAGGACCTAAGCCTTTTCCTCAGCACCCGCTGTGTCGTGGTGCTG
  CTGTCCGCGGAGCTGGTGCAGCACTTCCACAAGCCCGCCTTGCTGCCCCTGCTGCAGAGAGCTTTCCATC
  CTCCGCACCGCGTGGTCAGGCTGCTCTGCGGCGTGCGGGACAGCGAGGAGTTCCTAGACTTCTTTCCAGA
  TTGGGCCCATTGGCAGGAGCTCACCTGTGACGATGAGCCAGAGACCTACGTGGCAGCTGTGAAAAAAGCC
  ATTTCCGAAGATTCTGGCTGTGACTCAGTCACTGACACTGAGCCTGAGGACGAGAAGGTTGTTTCCTACT
  CGAAGCAGCAGAACCTGCCGACGGTGACTTCACCTGGGAACCTGATGGTGGTGCAGCCGGACCGCATTCG
  CTGTGGGGCAGAAACCACTGTCTATGTTATTGTGAGATGTAAGCTGGATGACAGGGTGGCGACAGAAGCA
  GAGTTTTCTCCTGAGGATTCTCCCTCTGTAAGGATGGAAGCCAAGGTGGAGAATGAGTACACCATTTCAG
  TGAAGGCTCCCAACCTTTCATCTGGGAACGTTTCTCTGAAGATATATTCTGGAGACTTAGTGGTGTGTGA
  AACCGTTATCAGCTATTATACTGACATGGAAGAAATTGGGAATTTATTGTCCAATGCCGCGAATCCTGTG
  GAATTCATGTGTCAGGCCTTTAAAATTGTGCCCTACAACACAGAGACCCTTGATAAACTGCTAACCGAAT
  CCCTGAAGAACAATATCCCTGCAAGCGGACTGCACCTCTTTGGAATCAACCAGCTGGAAGAAGAAGATAT
  GATGACAAATCAGAGGGATGAAGAGCTGCCCACCCTGTTGCATTTTGCTGCGAAGTATGGACTGAAGAAC
  CTCACTGCCTTGTTGCTCACCTGCCCAGGAGCCCTGCAGGCGTACAGCGTGGCCAACAAGCATGGCCACT
  ACCCCAACACCATCGCTGAGAAACACGGCTTCAGGGACCTGCGGCAGTTCATCGACGAGTATGTGGAAAC
  GGTGGACATGCTCAAGAGTCACATTAAAGAGGAACTGATGCACGGGGAGGAGGCTGATGCTGTGTACGAG
  TCCATGGCCCACCTTTCCACAGACCTGCTTATGAAATGCTCGCTCAACCCCGGCTGTGACGAGGATCTCT
  ATGAGTCCATGGCTGCCTTTGTCCCAGCTGCCACTGAAGACCTCTATGTTGAAATGCTTCAGGCCAGTAC
  ATCTAACCCAATCCCTGGAGATGGTTTCTCTCGGGCCACTAAGGACTCTATGATCCGCAAGTTTTTAGAA
  GGCAACAGCATGGGAATGACCAATCTGGAGAGAGATCAGTGCCATCTTGGTCAGGAAGAAGATGTTTATC
  ACACGGTGGATGACGATGAGGCCTTTTCTGTGGACCTGGCCAGCAGGCCCCCTGTCCCAGTGCCCAGACC
  AGAGACCACTGCTCCTGGTGCTCACCAGCTGCCTGACAACGAACCATACATTTTTAAAGTTTTTGCAGAA
  AAAAGTCAAGAGCGGCCTGGGAATTTCTACGTTTCCTCAGAGAGCATCAGGAAAGGGCCGCCCGTCAGAC
  CATGGAGGGACAGGCCCCAGTCGAGTATATATGACCCTTTTGCGGGAATGAAAACGCCAGGCCAGCGGCA
  GCTTATCACCCTCCAGGAGCAGGTGAAGCTGGGCATTGTCAACGTGGATGAGGCTGTGCTCCACTTCAAA
  GAGTGGCAGCTCAACCAGAAGAAACGATCGGAGTCCTTTCGTTTCCAGCAGGAAAATCTTAAACGGCTAA
  GAGACAGCATCACCCGAAGACAGAGAGAGAAGCAAAAATCAGGAAAGCAGACAGACTTGGAGATCACGGT
  CCCAATTCGGCACTCACAGCACCTGCCTGCAAAAGTGGAGTTTGGAGTCTATGAGAGTGGCCCCAGGAAA
  AGTGTCATTCCCCCTAGGACGGAGCTGAGACGAGGAGACTGGAAAACAGACAGCACCTCCAGCACAGCAA
  GTAGCACAAGTAACCGCTCCAGCACCCGGAGCCTCCTCAGTGTGAGCAGCGGGATGGAAGGGGACAACGA
  GGATAATGAAGTCCCTGAGGTTACCAGAAGTCGCAGTCCAGGCCCCCCACAAGTGGATGGGACACCCACC
  ATGTCCCTCGAGAGACCCCCCAGGGTGCCTCCGAGAGCTGCCTCACAGAGGCCTCCGACCAGGGAGACCT
  TCCATCCTCCTCCACCTGTTCCACCCAGAGGACGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214125 representing NM_152309
  Red=Cloning site Green=Tags(s)
  
  MAASGVPRGCDILIVYSPDAEEWCQYLQTLFLSSRQVRSQKILTHRLGPEASFSAEDLSLFLSTRCVVVL
  LSAELVQHFHKPALLPLLQRAFHPPHRVVRLLCGVRDSEEFLDFFPDWAHWQELTCDDEPETYVAAVKKA
  ISEDSGCDSVTDTEPEDEKVVSYSKQQNLPTVTSPGNLMVVQPDRIRCGAETTVYVIVRCKLDDRVATEA
  EFSPEDSPSVRMEAKVENEYTISVKAPNLSSGNVSLKIYSGDLVVCETVISYYTDMEEIGNLLSNAANPV
  EFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEEDMMTNQRDEELPTLLHFAAKYGLKN
  LTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKSHIKEELMHGEEADAVYE
  SMAHLSTDLLMKCSLNPGCDEDLYESMAAFVPAATEDLYVEMLQASTSNPIPGDGFSRATKDSMIRKFLE
  GNSMGMTNLERDQCHLGQEEDVYHTVDDDEAFSVDLASRPPVPVPRPETTAPGAHQLPDNEPYIFKVFAE
  KSQERPGNFYVSSESIRKGPPVRPWRDRPQSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFK
  EWQLNQKKRSESFRFQQENLKRLRDSITRRQREKQKSGKQTDLEITVPIRHSQHLPAKVEFGVYESGPRK
  SVIPPRTELRRGDWKTDSTSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEVPEVTRSRSPGPPQVDGTPT
  MSLERPPRVPPRAASQRPPTRETFHPPPPVPPRGR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152309
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152309.2, NP_689522.2
• RefSeq Size: 4817
• RefSeq ORF: 2418
• Locus ID: 118788
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway
• Gene Summary: Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. [UniProtKB/Swiss-Prot Function]