LHX6 (NM_199160) Human Tagged ORF Clone

CAT#: RC214132

LHX6 (Myc-DDK-tagged)-Human LIM homeobox 6 (LHX6), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404753) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214132 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LHX6
• Synonyms: LHX6.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214132 representing NM_199160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTGGAAGCATGAGAACGCCGCCCCGGCGTTGCCCGAGGGCTGCCGGCTGCCGGCCGAGGGCGGCC
  CCGCCACCGACCAGGTGATGGCCCAGCCAGGGTCCGGCTGCAAAGCGACCACCCGCTGTCTTGAAGGGAC
  CGCGCCGCCCGCCATGGCTCAGTCTGACGCCGAGGCCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAG
  GCCTCCCCATGTACGCCCAGCACGCCATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTG
  CAGGCAAGAACATCTGCTCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCT
  CATCTGGCACGTGCGGTGCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCTGCTAC
  ATCAAGAACAAGGAGATCTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCAAGTGTGCCCGGTGCG
  GCCGACAGATCTACGCCAGCGACTGGGTGCGGAGAGCTCGCGGCAACGCCTACCACCTGGCCTGCTTCGC
  CTGCTTCTCGTGCAAGCGCCAGCTGTCCACTGGTGAGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGC
  CGCATCCACTACGACACCATGATTGAGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGG
  AGGGGGCAGTGCCCTCGGAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGC
  GGAACAGCTGCAGGTTATGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGCAGAAG
  CTGGCGGACATGACGGGCCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACTGCCGGGCGCGTCATA
  AAAAGCACACGCCGCAACACCCAGTGCCGCCCTCGGGGGCGCCCCCGTCCCGCCTTCCCTCCGCCCTGTC
  CGACGACATCCACTACACCCCGTTCAGCAGCCCCGAGCGGGCGCGCATGGTCACCCTGCACGGCTACATT
  GAGAGTCATCCTTTTTCAGTACTAACGCTGCCGGCACTTCCGCATCTGCCCGTGGGCGCCCCACAGCTGC
  CCCTCAGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214132 representing NM_199160
  Red=Cloning site Green=Tags(s)
  
  MYWKHENAAPALPEGCRLPAEGGPATDQVMAQPGSGCKATTRCLEGTAPPAMAQSDAEALAGALDKDEGQ
  ASPCTPSTPSVCSPPSAASSVPSAGKNICSSCGLEILDRYLLKVNNLIWHVRCLECSVCRTSLRQQNSCY
  IKNKEIFCKMDYFSRFGTKCARCGRQIYASDWVRRARGNAYHLACFACFSCKRQLSTGEEFGLVEEKVLC
  RIHYDTMIENLKRAAENGNGLTLEGAVPSEQDSQPKPAKRARTSFTAEQLQVMQAQFAQDNNPDAQTLQK
  LADMTGLSRRVIQVWFQNCRARHKKHTPQHPVPPSGAPPSRLPSALSDDIHYTPFSSPERARMVTLHGYI
  ESHPFSVLTLPALPHLPVGAPQLPLSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199160
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_199160.2, NM_199160.3, NP_954629.2
• RefSeq Size: 3273
• RefSeq ORF: 1134
• Locus ID: 26468
• Protein Families: Transcription Factors
• MW: 41.1 kDa
• Gene Summary: This gene encodes a member of a large protein family that contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein has tandem LIM domains as well as a DNA-binding homeodomain. The protein functions as a transcription factor involved in embryogenesis and head development and is highly expressed in neural crest derived mesenchyme cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]