PIGA (NM_002641) Human Tagged ORF Clone
CAT#: RC214528L3
- LentiORF®
Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1, Myc-DDK-tagged
"NM_002641" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PIGA |
Synonyms | GPI3; MCAHS2; PIG-A; PNH1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC214528).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_002641 |
ORF Size | 1452 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002641.1, NP_002632.1 |
RefSeq Size | 3644 bp |
RefSeq ORF | 1455 bp |
Locus ID | 5277 |
Cytogenetics | Xp22.2 |
Domains | Glycos_transf_1 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
MW | 54.1 kDa |
Gene Summary | 'This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109567 | PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1 |
USD 760.00 |
|
RC214528 | PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1 |
USD 420.00 |
|
RG214528 | PIGA (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1 |
USD 460.00 |
|
RC214528L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1, mGFP tagged |
USD 620.00 |
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