PIGA (NM_002641) Human Untagged Clone

CAT#: SC109567

PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGA
• Synonyms: GPI3; MCAHS2; PIG-A; PNH1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002641, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTGTAGAGGAGGAGCTGGGAATGGCCACCGTGCCTCAGCTACACTCTCTCGGGTTAGCCCTGGAA
  GTCTTTACACATGTAGAACCCGTACCCATAATATATGCATGGTATCTGACTTTTTCTACCCAAATATGGG
  AGGCGTGGAAAGCCACATTTACCAGCTCTCTCAGTGCCTGATTGAAAGAGGGCATAAGGTTATAATTGTC
  ACCCATGCTTATGGAAATCGAAAAGGCATCCGTTACCTCACCAGTGGCCTCAAAGTCTATTACTTGCCTC
  TGAAAGTCATGTACAACCAGTCTACAGCCACGACCCTCTTTCACAGTCTGCCATTGCTCAGGTACATATT
  TGTTCGGGAGAGAGTCACGATAATCCATTCACATAGTTCTTTTTCTGCTATGGCCCATGATGCTCTCTTC
  CACGCCAAGACAATGGGGCTTCAGACAGTCTTCACGGACCATTCCCTTTTTGGATTTGCTGATGTCAGCT
  CGGTGCTTACAAACAAGCTTCTAACCGTGTCTCTTTGTGATACAAACCACATCATTTGTGTGTCTTATAC
  TAGTAAGGAAAATACTGTACTAAGAGCAGCACTGAATCCTGAAATAGTGTCCGTCATTCCTAATGCTGTA
  GATCCTACTGACTTCACTCCAGACCCATTTAGAAGGCATGATAGTATAACTATTGTTGTTGTCAGCAGAC
  TTGTTTACAGAAAAGGGATCGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATATCCAGATTT
  AAATTTCATAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTTCGGGAAAGATACCAGCTG
  CATGACAGGGTGCGTCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAATGTCTTAGTTCAAGGACATA
  TTTTTCTGAATACCTCCCTTACTGAAGCATTCTGCATGGCGATCGTGGAAGCAGCCAGTTGTGGTTTACA
  GGTTGTAAGTACCAGAGTTGGTGGAATTCCTGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCT
  TCAGTAAAATCTTTGTGTGAAGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGCCAGCTC
  CAGAAAACATCCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAGAAAGAACTGAAAAGGT
  ATATGACCGGGTATCAGTGGAAGCTGTGTTGCCAATGGACAAACGACTGGACAGACTTATTTCTCACTGC
  GGCCCAGTAACAGGCTACATCTTTGCTTTGTTGGCAGTTTTCAACTTCCTCTTCCTCATTTTCTTGAGAT
  GGATGACTCCAGATTCTATCATTGATGTTGCAATAGATGCCACTGGGCCACGGGGTGCCTGGACTAATAA
  CTATTCTCACAGTAAAAGAGGGGGTGAGAATAATGAGATATCTGAAACCAGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002641 unedited
  GGTTCACATTAGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGGGNACACT
  GNGCGGCCATGGNAACTCACCGGTAATAGAGGACACATCTCTTAACTGGGTTGCTCTAAG
  AACTGATGTCTAAACCGTCTCAGCATGGCCTGTAGAGGAGGAGCTGGGAATGGCCACCGT
  GCCTCAGCTACACTCTCTCGGGGATCGATTTGCTTAGTGGTATAATACCTGAACTCTGTC
  AGAAATATCCAGATTTAAATTTCATAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGG
  AAGAAGTTCGGGAAAGATACCAGCTGCATGACAGGGTGCGTCTTTTGGGAGCTTTAGAAC
  ACAAGGATGTTAGAAATGTCTTAGTTCAAGGACATATTTTTCTGAATACCTCCCTTACTG
  AAGCATTCTGCATGGCGATCGTGGAAGCAGCCAGTTGTGGTTTACAGGTTGTAAGTACCA
  GAGTTGGTGGAATTCCTGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCTTCAG
  TAAAATCTTTGTGTGAAGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGC
  CAGCTCCATAAAACATCCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAC
  AAAGAACTGAAAAAGTATATGACCGGGTATCAGCGGAAGCTGTGCTGCCAATGGACAAAC
  GACTGGACAGACTTATTTTCACTGTGGGCCCATAACAGGCTACATCTTTGCCTTGTTGGC
  AGGTTTTCACTTCCTCTTCCTCATTTCTTGAGATGGAGACTCCCGATTCTATCCTTGGAT
  GTTGCAATAAATGCCACTGGGCCCCCCGGGTGCCCTGGACTAATACCTATTTTCCCAGAC
  ATAAGAGGGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002641 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCAAAATGTCATTCTGGTCT
  TTATTTTTGGACATGTAGCATGTTTTAACAAATCAGTTTTTCATAGGCAACCTTTTGAAA
  CATCAAAAGAAATACAATATATTTTTCACAAATTTCTCATCACTGTAAATTCACTTTAAA
  ATCAACTAAGTAGCAGGGTAGAGCAATGTGTTTCCATATACCGACCAGTGCAAACTGACA
  GTTACAATTAAGGTATCCAGAAAGGTTGAAAATTAAGATTTACGTGAAACAAATTTACTT
  CCATTTGTTAAAAAAAAGAAAAATAATAATTTGCAAATCACATGTTTTACAGGCAAAGGC
  AACTAACTTTAGATCTTATTTCCATCTCAAATAAATGACAGTTTAAGAATTAAATGACAA
  TTTAAAAAAATTAAGCAAAAATATAAGGAAAAAATGGTGGCATGCCTCTAAAACCTGTTG
  AATAGAATAATGGCCAAATATTACAGTTTCTCACTTTCCTATGAATACTGGCACTGTTTA
  TTTCATGTTTATATGTGAGTTTCTATGCATAAAAATCCCAGTAAGACTGAATAGTTTAAA
  GATCAGTCCATTTTTCTCCAACAAAACCCCTAACTTCTAGATTTTAAAAATTGCACTCTT
  GCTTTTGCATTTTAGTACAAACAAAACACAAATATATTTCTTTTATATCAGTGCAACCAG
  TTAATAGGCATGGTATTTTTTAAAAAGTAATAAAATTTGTGAATGGTGTCATTNTTAAAA
  ATCCTATACAGAATCANCACACTGTCAGAATCTATGTAGTGCACCCACAGTGCCTNNATG
  TTTGAATNACAGTCCTTTTCTACANAACCTTNAGAGTCAAGATNCATATACCACATGTGG
  TAANTGGGGGTAAAACCCTGCTCTGGCACACATGACGTATAACTCACCCAGACGTNCTAA
  TTNCACTAGTGCATATACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002641
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002641.1, NP_002632.1
• RefSeq Size: 3589 bp
• RefSeq ORF: 1455 bp
• Locus ID: 5277
• Cytogenetics: Xp22.2
• Domains: Glycos_transf_1
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).